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01-06-2013 | Translational Neurosciences - Short communication

A comparison between radiometric and fluorimetric methods for measuring SSAO activity

Authors: Alejandro Gella, Montse Solé, Irene Bolea, Mariacarla Ventriglia, Mariacristina Siotto, Nuria Durany, Rosanna Squitti, Mercedes Unzeta

Published in: Journal of Neural Transmission | Issue 6/2013

Abstract

Semicarbazide-sensitive amine oxidase (SSAO) metabolizes the oxidative deamination of primary aromatic and aliphatic amines. The final cytotoxic products of its catalysis contribute to diseases involving vascular degeneration. The increasing interest in measuring SSAO activity has led to the development of several different methods. Herein, we compare SSAO activity results obtained with radiometric and fluorimetric methods in 49 plasma samples. Although not interchangeable, a significant correlation was obtained between methods. Considering these limitations, the fluorimetric method might replace the radioisotopic one.

Abella A, Garcia-Vicente S, Viguerie N, Ros-Baro A, Camps M, Palacin M, Zorzano A, Marti L (2004) Adipocytes release a soluble form of VAP-1/SSAO by a metalloprotease-dependent process and in a regulated manner. Diabetologia 47:429–438PubMedCrossRef

Bland JM, Altman DG (1986) Statistical methods for assessing agreement between two methods of clinical measurement. Lancet 1:307–310PubMedCrossRef

Boomsma F, Derkx FH, van den MeirackerAH Man, Man in’t Veld AJ, Schalekamp MA (1995) Plasma semicarbazide-sensitive amine oxidase activity is elevated in diabetis mellitus and correlates with glycosylated haemoglobin. Clin Sci (Lond) 88:675–679

Boomsma F, van Veldhuisen DJ, de Kam PJ, Man in’t Veld AJ, Mosterd A, Lie KI, Schalekamp MA (1997) Plasma semicarbazide-sensitive amine oxidase is elevated in patients with congestive heart failure. Cardiovasc Res 33:387–391PubMedCrossRef

Castillo V, Lizcano JM, Visa J, Unzeta M (1998) Semicarbazide-sensitive amine oxidase (SSAO) from human and bovine cerebrovascular tissues: biochemical and immunohistological characterization. Neurochem Int 33:415–423PubMedCrossRef

Ferrer I, Lizcano JM, Hernández M, Unzeta M (2002) Overexpression of semicarbazide sensitive amine oxidase in the cerebral blood vessels in patients with Alzheimer’s disease and cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy. Neurosci Lett 321:21–24PubMedCrossRef

Hernandez-Guillamon M, Garcia-Bonilla L, Solé M, Sosti V, Parés M, Campos M, Ortega-Aznar A, Domínguez C, Rubiera M, Ribó M, Quintana M, Molina CA, Alvarez-Sabín J, Rosell A, Unzeta M, Montaner J (2010) Plasma VAP-1/SSAO activity predicts intracranial hemorrhages and adverse neurological outcome after tissue plasminogen activator treatment in stroke. Stroke 41(7):1528–1535PubMedCrossRef

Hernández-Guillamón M, Solé M, Delgado P, García-Bonilla L, Giralt D, Boada C, Penalba A, García S, Flores A, Ribó M, Alvarez-Sabin J, Ortega-Aznar A, Unzeta M, Montaner J (2012) VAP-1/SSAO plasma activity and brain expression in human hemorrhagic stroke. Cerebrovasc Dis 33(1):55–63PubMedCrossRef

Karádi I, Mészáros Z, Attila Csányi A, Szombathy T, Hosszú falusi N, Romics L, Magyar K (2002) Serum semicarbazide-sensitive amine oxidase (SSAO) activity is an independent marker of carotid atherosclerosis. Clin Chim Acta 323:139–146PubMedCrossRef

Kurkijärvi R, Yegutkin GG, Gunson BK, Jalkanen S, Salmi M, Adams DH (2000) Circulating soluble vascular adhesion protein 1 accounts for the increased sèrum monoamine oxidase activity in chronic liver disease. Gastroenterology 119:1096–1103PubMedCrossRef

Lewinsohn R (1984) Mammalian monoamine-oxidizing enzymes, with special reference to benzylamine oxidase in human tissues. Braz J Med Biol Res 17:223–256PubMed

del Mar Hernandez M, Esteban M, Szabo P, Boada M, Unzeta M (2005) Human plasma semicarbazide sensitive amine oxidase (SSAO), beta-amyloid protein and aging. Neurosci Lett 384:183–187PubMedCrossRef

Mészáros Z, Szombathy T, Raimondi L, Kara′di I, Romics L, Magyar K (1999) Elevated serum semicarbazide-sensitive amine oxidase activity in non-insulin-dependent diabetes mellitus: correlation with body mass index and serum triglyceride. Metabolism 48:113–117PubMedCrossRef

O’Sullivan J, Unzeta M, Healy J, O’Sullivan MI, Davey G, Tipton KF (2004) Semicarbazide-sensitive amine oxidases: enzymes with quite a lot to do. Neurotoxicology 25:303–315PubMedCrossRef

Otsuka S, Kobayashi Y (1964) Radioisotopic assay for monoamine oxidase determinations in human plasma. Biochem Pharmacol 13:995–1006PubMedCrossRef

Passing H, Bablok W (1983) A new biometrical procedure for testing the equality of measurements from two different analytical methods. Application of linear regression procedures for method comparison studies in clinical chemistry, Part I. J Clin Chem Clin Biochem 21:709–720PubMed

Peet GW, Lukas S, Hill-Drzewi M, Martin L, Rybina IV, Roma T, Shoultz A, Zhu X, Cazacu D, Kronkaitis A, Baptiste A, Raudenbush BC, August EM, Modis LK (2011) Bioluminescent method for assaying multiple semicarbazide-sensitive amine oxidase (SSAO) family members in both 96- and 384-well formats. J Biomol Screen 16(9):1106–1111PubMedCrossRef

Salmi M, Jalkanen S (2006) Developmental regulation of the adhesive and enzymatic activity of vascular adhesion protein-1 (VAP-1) in humans. Blood 108(5):1555–1561PubMedCrossRef

Valente T, Gella A, Solé M, Durany N, Unzeta M (2012) Immunohistological study of semicarbazide-sensitive amine oxidase/vascular adhesion protein-1 in the hippocampal vasculature: pathological synergy of Alzheimer’s disease and diabetes mellitus. J Neurosci Res 90(10):1989–1996PubMedCrossRef

Yraola F, García-Vicente S, Fernandez-Recio J, Albericio F, Zorzano A, Marti L, Royo M (2006) New efficient substrates for semicarbazide-sensitive amine oxidase/vap-1 enzyme: analysis by sars and computational docking. J Med Chem 49:6197–6208PubMedCrossRef

Title: A comparison between radiometric and fluorimetric methods for measuring SSAO activity
Authors: Alejandro Gella
Montse Solé
Irene Bolea
Mariacarla Ventriglia
Mariacristina Siotto
Nuria Durany
Rosanna Squitti
Mercedes Unzeta
Publication date: 01-06-2013
Publisher: Springer Vienna
Published in: Journal of Neural Transmission / Issue 6/2013
Print ISSN: 0300-9564
Electronic ISSN: 1435-1463
DOI: https://doi.org/10.1007/s00702-013-0987-z

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A comparison between radiometric and fluorimetric methods for measuring SSAO activity

Abstract

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