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Lasers in Medical Science
Published in: Lasers in Medical Science 4/2016

01-05-2016 | Original Article

808-nm laser therapy with a flat-top handpiece photobiomodulates mitochondria activities of Paramecium primaurelia (Protozoa)

Authors: Andrea Amaroli, Silvia Ravera, Steven Parker, Isabella Panfoli, Alberico Benedicenti, Stefano Benedicenti

Published in: Lasers in Medical Science | Issue 4/2016

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Abstract

Photobiomodulation is proposed as a non-linear process, and only low-level laser therapy (LLLT) is assumed to stimulate exposed cells, whereas high powered laser and fluences can cause negative effects, exhausting the cell’s energy reserve as a consequence of excessive photon-based stimulation. In our work, we investigated and compared the effects of 808-nm diode laser (CW) with a new flat-top handpiece. To this purpose, we tested the photobiomodulation effects of 1 and 3 J/cm2 fluence, both generated by 100 mW or 1 W of laser power and of 64 J/cm2 of fluence generated by 100 mW, 1 W, 1.5 W or 2 W, as expressed through oxygen consumption and ATP synthesis of Paramecium. Data collected indicates the incremental consumption of oxygen through irradiation with 3 J/cm2–100 mW or 64 J/cm2–1 W correlates with an increase in Paramecium ATP synthesis. The Paramecium respiration was inhibited by fluences 64 J/cm2–100 mW or 64 J/cm2–2 W and was followed by a decrease in the endogenous ATP concentration. The 1 J/cm2–100 mW or 1 W and 3 J/cm2–1 W did not affect mitochondrial activity. The results show that the fluence of 64 J/cm2–1 W more than the 3 J/cm2–100 mW causes greater efficiency in Paramecium mitochondria respiratory chain activity. Our results suggest that thanks to flat-top handpiece we used, high fluences by high-powered laser have to be reconsidered as an effective and non-invasive therapy. Possible associated benefits of deeper tissue penetration would increase treatment effectiveness and reduced irradiation time.
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Metadata
Title
808-nm laser therapy with a flat-top handpiece photobiomodulates mitochondria activities of Paramecium primaurelia (Protozoa)
Authors
Andrea Amaroli
Silvia Ravera
Steven Parker
Isabella Panfoli
Alberico Benedicenti
Stefano Benedicenti
Publication date
01-05-2016
Publisher
Springer London
Published in
Lasers in Medical Science / Issue 4/2016
Print ISSN: 0268-8921
Electronic ISSN: 1435-604X
DOI
https://doi.org/10.1007/s10103-016-1901-3

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