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BMC Cancer
Published in: BMC Cancer 1/2009

Open Access 01-12-2009 | Research article

δ-ALA-D activity is a reliable marker for oxidative stress in bone marrow transplant patients

Authors: Thissiane L Gonçalves, Dalila M Benvegnú, Gabriela Bonfanti, Andressa V Frediani, João Batista T Rocha

Published in: BMC Cancer | Issue 1/2009

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Abstract

Background

Bone marrow transplantation (BMT) is often used in the treatment of various diseases. Before BMT, patients are submitted to a conditioning regimen (CR), which consists of the administration of high doses of chemotherapy. The action of many cytostatic drugs involves the overproduction of reactive oxygen species, which together with inadequate antioxidant protection can lead to oxidative stress and this has been implicated in the etiology of various diseases. The objectives of this study were to look for evidence of oxidative stress and also to analyze δ-Aminolevulinato dehydratase (δ-ALA-D) activity as a possible marker of oxidative stress in autologous and allogeneic BMT patients.

Methods

Lipid peroxidation, vitamin C and thiol group levels as well as catalase, superoxide dismutase and δ-ALA-D activity were determined in 37 healthy controls, 13 patients undergoing autologous peripheral blood stem cell transplantation and 24 patients undergoing allogeneic BMT.

Results

We found that patients presented signs of oxidative stress before they were submitted to BMT, during CR and up to 20 days after BMT. There was a decrease in enzymatic and non enzymatic antioxidant defenses, in δ-ALA-D activity, and an increase in lipoperoxidation in the blood of both patient groups.

Conclusion

This study has indicated that autologous and allogeneic BMT are associated with oxidative stress. Moreover, blood δ-ALA-D activity seems to be an additional biomarker of oxidative stress in BMT patients.
Appendix
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Metadata
Title
δ-ALA-D activity is a reliable marker for oxidative stress in bone marrow transplant patients
Authors
Thissiane L Gonçalves
Dalila M Benvegnú
Gabriela Bonfanti
Andressa V Frediani
João Batista T Rocha
Publication date
01-12-2009
Publisher
BioMed Central
Published in
BMC Cancer / Issue 1/2009
Electronic ISSN: 1471-2407
DOI
https://doi.org/10.1186/1471-2407-9-138

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