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01-08-2011 | Basic Neurosciences, Genetics and Immunology - Original Article

α-Synuclein increases U251 cells vulnerability to hydrogen peroxide by disrupting calcium homeostasis

Authors: Zhengxin Ying, Fankai Lin, Weihong Gu, Yang Su, Abida Arshad, Hong Qing, Yulin Deng

Published in: Journal of Neural Transmission | Issue 8/2011

Abstract

Multiple system atrophy (MSA) is a progressive neurodegenerative disease characterized by glial cytoplasmic inclusions containing insoluble α-synuclein. Since Ca²⁺ plays an important role in cell degeneration, [Ca²⁺]_i in α-synuclein-overexpressed human glioma cells was analyzed by Fura-2 fluorometry. Overexpression of α-synuclein increased the basal level of [Ca²⁺]_i, and a higher Ca²⁺ response to hydrogen peroxide was further observed. The effect that α-synuclein overexpression caused U251 cells to be more vulnerable to hydrogen peroxide was eliminated by Ca²⁺ chelator BAPTA-AM or transient receptor potential channels blocker SKF 96365 but not by L-type Ca²⁺ channel blocker nimodipine. These findings suggest that the dysregulation of cellular Ca²⁺ homeostasis caused by α-synuclein under oxidative stress may contribute to the glial cell death in MSA.

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Title: α-Synuclein increases U251 cells vulnerability to hydrogen peroxide by disrupting calcium homeostasis
Authors: Zhengxin Ying
Fankai Lin
Weihong Gu
Yang Su
Abida Arshad
Hong Qing
Yulin Deng
Publication date: 01-08-2011
Publisher: Springer Vienna
Published in: Journal of Neural Transmission / Issue 8/2011
Print ISSN: 0300-9564
Electronic ISSN: 1435-1463
DOI: https://doi.org/10.1007/s00702-011-0596-7

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α-Synuclein increases U251 cells vulnerability to hydrogen peroxide by disrupting calcium homeostasis

Abstract

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