황기, 지치 복합물의 연골세포에서의 Matrix Metalloproteinases 저해 효과 및 유효성분의 분석

최두진; 최보람; 이대영; 최수임; 이영섭; 김금숙

doi:10.7783/KJMCS.2019.27.4.247

주제분류

...

저널정보

한국약용작물학회
한국약용작물학회지 학술저널
한국약용작물학회지 제27권 제4호
2019.8 247 - 258 (12page)
DOI : 10.7783/KJMCS.2019.27.4.247

저자정보

최두진 (농촌진흥청)
최보람 (농촌진흥청)
이대영 (농촌진흥청)
최수임 (덕성여자대학교)
이영섭 (농촌진흥청)

이용수
내서재: 0

내서재에 추가
되었습니다. 내서재에서
삭제되었습니다.

초록·키워드

오류제보하기

Background: Astragali radix (A) and Lithospermi radix (L) have long been used as traditional medicines due to their known anti-inflammatory effects. This study aimed at evaluating, their optimal mixing ratio and their functional compounds by investigating the inhibitory effects of mixed extracts of A and L and their active compounds on matrix metalloproteinases (MMPs).
Methods and Results: A and L extracts were obtained by extraction at 80℃ using 50% and 70% fermented alcohol, respectively, and then mixed at a ratio of 5 : 5, 6 : 4, 7 : 3 and 8 : 2 (w/w). The activities of MMP-1, MMP-3, and MMP-13 were evaluated in interleukin-1beta (IL-1β)-induced SW1353 cells. The extract mixtures showed synergistic inhibitory effects on MMP-3 and MMP-13, higher than the effects of the individual A and L extracts. The 7 : 3 mixture (ALM16) showed the most effective MMPs inhibitory activity, while among the active ingredients, calycosin-7-O-β-D-glucoside and lithospermic acid exhibited excellent MMPs inhibitory activity. Additionally, an HPLC method was established for simultaneous quantification of the effective components of the extract mixtures, and validated by measuring the linearity, precision and accuracy of the limit of detection (LOD) and limit of quantification (LOQ).
Conclusions: ALM16 showed the most effective MMPs inhibitory activity. Calycosin-O-β-D-glucoside, calycosin and lithospermic acid were identified as useful candidates, as they were the major functional compounds in the MMP inhibitory activity. Summarily, ALM16 might be a highly effective in osteoarthritis management, owing to its because it exhibits a protective effect on cartilage via excellent inhibition of MMPs.

#Astragali Radix #Lithospermi Radix #Matix Metalloproteinases (MMPs)