Correlations of Bcl-2 and Survivin Gene Protein Expressions in Colorectal Cancer

Yue Ma; Hong Sheng Wang

doi:10.4028/www.scientific.net/AMM.423-426.362

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HomeApplied Mechanics and MaterialsApplied Mechanics and Materials Vols. 423-426Correlations of Bcl-2 and Survivin Gene Protein...

Correlations of Bcl-2 and Survivin Gene Protein Expressions in Colorectal Cancer

Abstract:

Objective: In this study, immunohistochemical streptavidinbiotin-peroxidase (S-P method) was used to detect the correlation of gene proteins related to apoptosis, such as Bcl-2 and Survivin in colorectal carcinoma. Methods: Pathological specimens from 46 colorectal cancer patients and 8 normal tissues around the tumor specimens were selected. Immunohistochemical streptavidin-biotin-peroxidase assay was used to detect the expression of Bcl-2 and Surviving protein. Results: In 25 cases of Bcl-2 positive expression, there were 24 cases of Survivin positive expression, accounting for 96%; while there were 8 cases of Survivin positive expression in 21 cases of Bcl-2 negative expression, accounting for 38.1%, showing the expression of Bcl-2 and the expression of Survivin was positively correlated (Pearsons R= 0.627, P<0.01). In 15 cases of well-differentiated colorectal cancer tissues, there were 6 Cases of Survivin positive expression, and the positive rate was 40%; in 17 cases of moderately differentiated colorectal cancer tissues, there were 17 cases, and the positive rate was 100%; in 14 cases of poorly differentiated colorectal cancer tissues, there were 9 cases, and the positive rate was 64.29%. The statistical analysis showed that the Survivin positive expression in the three colorectal cancer tissues presented a significant difference (P <0.01). Results: The positive expression of Survivin protein was directly correlated to that of Bcl-2, which suggested that both of them acted on different stages of apoptosis to promote jointly the development of colorectal cancer in a synergistic way.

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Periodical:

Applied Mechanics and Materials (Volumes 423-426)

Pages:

362-365

DOI:

https://doi.org/10.4028/www.scientific.net/AMM.423-426.362

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Online since:

September 2013

Authors:

Yue Ma, Hong Sheng Wang

Keywords:

Bcl-2, Colorectal Cancer, Survivin Protein

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