Baseline biological growth data were obtained under laboratory conditions for four local populations of the phlebotomine sand fly P. papatasi (Scopoli, 1786) (Diptera : Psychodidae) in different eco-regions at altitudes between 368 and 1117 m in the Sanliurfa province of Turkey. The developmental time from egg to adult was found to be significantly different among the populations: 36 days for the AKL population (368 m), 43 days for the HHR population (488 m), 45 days for the HMD population (644 m), and 49 days for the ALT population (1117 m), respectively. Based on cohorts of adults in each population, horizontal life tables were constructed. The average longevity was determined to be in the range of 8.75±2.39 to 11.60±3.48 days for adult females, and it was found to be slightly longer for adult males. No significant difference was found in life expectancy at emergence, e_x when x=1, between females and males in general (P>0.05) in all the populations. While significant differences could be demonstrated among populations for predictive parameters such as net reproductive rate, R_o, and generation time, T_c, no significant differences among the populations were found in terms of intrinsic rate of increase, r_m, finite rate of increase, λ, birth (b) and death (d) rates (P>0.05). Populations that produced offspring earlier in life also produced more total female offspring, since T_c was negatively correlated with Ro among the populations (r = −0.686, 0.01<P<0.05). Twenty-seven parameters in all life stages, both pre-adult and adult features of P. papatasi, were used as physiological variables and these operational taxonomic units were analyzed using Principal Component Analysis. Analyses confirmed results from the previous morphometric and molecular studies that the Alitas population orientated and clustered as a distinct group along the first two PCs.