pISSN 1229-6929
eISSN 2005-8330
Open Access, Peer-reviewed
    Korean J Radiol. 2017 Jul-Aug;18(4):739-748. English.
    Published online May 19, 2017.
    https://doi.org/10.3348/kjr.2017.18.4.739
    Copyright © 2017 The Korean Society of Radiology
    Original Article

    CT Quantification of Lungs and Airways in Normal Korean Subjects

    Song Soo Kim, MD,1 Gong Yong Jin, MD,2 Yuan Zhe Li, MD,3 Jeong Eun Lee, MD,1 and Hye Soo Shin, MD1
      • 1Department of Radiology, Chungnam National University Hospital, Chungnam National University School of Medicine, Daejeon 35015, Korea.
      • 2Department of Radiology, Research Institute of Clinical Medicine of Chonbuk National University-Biomedical Research Institute of Chonbuk National University Hospital, Chonbuk National University Medical School, Institute of Medical Science, Jeonju 54907, Korea.
      • 3Department of Radiology, Research Institute of Clinical Medicine of Chonbuk National University-Biomedical Research Institute of Chonbuk National University Hospital, Jeonju 54907, Korea.
    • Corresponding author: Gong Yong Jin, MD, Department of Radiology, Research Institute of Clinical Medicine of Chonbuk National University-Biomedical Research Institute of Chonbuk National University Hospital, Chonbuk National University Medical School, Institute of Medical Science, 20 Geonji-ro, Deokjin-gu, Jeonju 54970, Korea. Tel: (8263) 250-2307, Fax: (8263) 272-0481, Email: gyjin@jbnu.ac.kr
    Received August 15, 2016; Accepted January 05, 2017.

    This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

    Abstract

    Objective

    To measure and compare the quantitative parameters of the lungs and airways in Korean never-smokers and current or former smokers (“ever-smokers”).

    Materials and Methods

    Never-smokers (n = 119) and ever-smokers (n = 45) who had normal spirometry and visually normal chest computed tomography (CT) results were retrospectively enrolled in this study. For quantitative CT analyses, the low attenuation area (LAA) of LAAI-950, LAAE-856, CT attenuation value at the 15th percentile, mean lung attenuation (MLA), bronchial wall thickness of inner perimeter of a 10 mm diameter airway (Pi10), total lung capacity (TLCCT), and functional residual capacity (FRCCT) were calculated based on inspiratory and expiratory CT images. To compare the results between groups according to age, sex, and smoking history, independent t test, one way ANOVA, correlation test, and simple and multiple regression analyses were performed.

    Results

    The values of attenuation parameters and volume on inspiratory and expiratory quantitative computed tomography (QCT) were significantly different between males and females (p < 0.001). The MLA and the 15th percentile value on inspiratory QCT were significantly lower in the ever-smoker group than in the never-smoker group (p < 0.05). On expiratory QCT, all lung attenuation parameters were significantly different according to the age range (p < 0.05). Pi10 in ever-smokers was significantly correlated with forced expiratory volume in 1 second/forced vital capacity (r = −0.455, p = 0.003). In simple and multivariate regression analyses, TLCCT, FRCCT, and age showed significant associations with lung attenuation (p < 0.05), and only TLCCT was significantly associated with inspiratory Pi10.

    Conclusion

    In Korean subjects with normal spirometry and visually normal chest CT, there may be significant differences in QCT parameters according to sex, age, and smoking history.

    Keywords
    Quantitative computed tomography; Lungs; Airways; Non-smokers; Smokers; Normal value; Pulmonary function test

    INTRODUCTION

    Given the limitations of spirometry in the characterization of chronic obstructive pulmonary disease (COPD), quantitative computed tomography (QCT) is increasingly being used to evaluate different COPD phenotypes, such as emphysema, large airway remodeling, and expiratory air trapping (1, 2, 3, 4, 5, 6). Spirometry may be an insensitive instrument partly owing to variations in maximal forced expiratory volume in 1 second (FEV1) as a buffer of the capacity prior to the detectable declining level in subclinical emphysema (7, 8, 9). However, even though QCT allows for more objective measurements, there are only a few reports regarding the QCT measurements in healthy normal subjects demonstrating the limitation of measurement variation according to age, height, sex, race/ethnicity, and scanner manufacturer (10, 11). Inspiratory low attenuation area (LAA) ranged from less than 1% to around 8% in the lung tissue with attenuation values of −950 Hounsfield units (HUs) or less on inspiratory CT (LAAI-950) in studies performed among non-smokers or normal subjects (12, 13). According to a recent QCT study among healthy non-Hispanic white non-smoking adults, the mean percentage of LAAI-950 was 2.0%, although the study did not mention the normal values of different airway parameters (10). Few efforts have been made to measure QCT parameters in normal Korean subjects; such data could be useful, supportive, reference data in the future. The purpose of this study was to measure the QCT parameters of the lungs and airways in Korean never-smokers and ever-smokers with normal spirometry and visually normal chest CT results and to compare the results according to age, sex, and smoking history.

    MATERIALS AND METHODS

    Study Subjects

    Between September 2013 and April 2015, a total of 1000 consecutive subjects (500 males and 500 females; 15 to 81 years old; mean age, 51.0 ± 16.1 years) who underwent chest CT and pulmonary function tests (PFTs) were recruited from two institutions. Among them, those with medical records showing normal PFT results, normal findings on chest CT scans, and no known history of lung disease or surgery were enrolled in this study. Subjects were excluded when chest CT protocols were not identical between the two institutions. Medical records prepared by fourth year radiology residents were retrospectively reviewed with a focus on physical examination, age, sex, smoking history, chest CT report, and PFT results. Finally, 164 subjects who had normal results on chest CT and spirometry tests and normal physical examination according to their medical records were selected for the analysis. One hundred nineteen subjects were never-smokers and 45 subjects were ex-smokers or current smokers. Their overall mean age was 53.3 ± 14.4 years. There were 67 males (mean age, 52.5 ± 14.8 years) and 97 females (mean age, 57.1 ± 13.8 years) and the proportion of ever-smokers among males and females was 66 percent and one percent, respectively. The Institutional Review Board of each institution approved this retrospective study and waived the requirement of obtaining informed consent.

    CT Examination, Acquisition

    All subjects underwent volumetric assessment using a 16 or 128 multidetector CT scanner (Somatom Sensation 16, Siemens Medical Solutions, Erlangen, Germany; Somatom Definition AS, Siemens Medical Solutions, Forchheim, Germany, respectively) without contrast in the craniocaudal direction while they were placed in the supine position; scans were taken at full inspiration and relaxed exhalation after coaching. Volumetric CT scans were obtained both at full inspiration (200 mAs) and at the end of normal expiration (50 mAs) using 120 kVp tube current, 0.5 or 0.33 second rotation time, submillimeter reconstruction (thickness: 0.75 mm, interval: 0.5 mm), B35f (or B31f) and B46f (or B45f) reconstruction kernel, and 16 × 0.75 or 128 × 0.6 mm acquisition. Images were displayed in the lung window to assess the lung parenchyma (window level, −600 to −700 HU; window width, 1200–1500 HU) (14).

    Quantitative CT Measurements and Data Analysis

    Whole lung volumetric CT images for quantitative analysis were reconstructed using a standard algorithm. Using commercial software (Pulmonary Workstation, version 2.0, VIDA Diagnostics, Coralville, IA, USA), QCT measurements for LAAI-950, measurements with an attenuation value of −856 HU or less on expiratory CT (LAAE-856), CT attenuation value at the 15th percentile (HU 15th percentile), mean lung attenuation (MLA), and airway wall thickness were obtained (5, 14, 15, 16, 17, 18, 19, 20). The software automatically calculated the LAA, which is defined as the percentage of lung voxels at or below the designated threshold value on inspiratory or expiratory CT. Quantitative assessment of airway wall thickness was performed automatically as the square root of the wall area at the inner perimeter of a 10 mm diameter airway (Pi10). Airway segmentations obtained with the software were assessed for complete airway inclusion as well as correct and consistent labeling. Skipped branch points were manually added as necessary to ensure accurate measurement of airway length. Total lung capacity (TLCCT) and functional residual capacity (FRCCT) were also calculated based on the inspiratory and expiratory CT images, respectively. Skewness and kurtosis were measured to evaluate the degree of pulmonary fibrosis.

    Pulmonary Function Testing

    Pulmonary function tests were performed according to the American Thoracic Society guidelines (21). A portable spirometer (Chestgraph HI-701, Chest Co. Ltd., Tokyo, Japan) was used, and the following values were assessed: vital capacity (VC), residual volume, TLC, FEV1, forced VC (FVC), FEV1/FVC ratio, forced expiratory flow 25–75%, and diffusion capacity of the lung for carbon monoxide. All PFTs were performed on the same day as chest CT scans.

    Statistical Analysis

    Results are expressed as mean ± standard deviation for continuous variables. In univariate analysis, differences in continuous data between the two groups were analyzed with a 2-tailed unpaired t test. The Wilcoxon rank-sum test was used for non-normally distributed data. One-way ANOVA was used to evaluate the difference between groups. Pearson's correlation coefficient was used to evaluate the relationship between QCT parameters and PFTs. Simple and multiple regression analyses were performed to identify the factors associated with lung attenuation and airway parameters. Statistical analyses were performed using SPSS version 12.0.1 (SPSS Inc., Chicago, IL, USA) and SAS version 9.3 (SAS Institute Inc., Cary, NC, USA). p values < 0.05 were considered statistically significant.

    RESULTS

    The baseline characteristics and QCT measurements of these 164 subjects are shown in Table 1. All 164 subjects showed normal PFT results; however, FEV1 (% predicted), FVC (% predicted), and the FEV1/FVC ratio in females were significantly greater than those in males (p < 0.05). The MLA and the 15th percentile on inspiratory QCT were significantly lower in males than in females (p < 0.001). However, %LAAI-950 and %LAAE-856 were not significantly different between males and females (p = 0.165 and 0.432, respectively). The values of inspiratory and expiratory Pi10 as assessed by QCT were significantly greater in males than in females (p < 0.001). TLCCT and FRCCT were also greater in males than in females (p < 0.001). Skewness and kurtosis were not significantly different between the genders. These significant differences in PFT results and QCT measurements between males and females among all subjects still remained even only in never-smokers (all p < 0.05 except for FEV1/FVC) (Table 2).

    Table 1
    Subject Characteristics, Physiologic and Quantitative Measurements

    Table 2
    Physiologic and Quantitative Measurements in Never-Smokers

    Comparison between the Never-Smoker and Ever-Smoker Groups in Terms of QCT and PFT

    Forty-five subjects were ever-smokers. There were no airflow limitations in the ever-smoker group, but FEV1 (% predicted) and FVC (% predicted) were significantly greater in the never-smoker group (p < 0.001) (Table 3). The MLA and HU 15th percentile on inspiratory QCT were significantly lower in the ever-smoker group than in the never-smoker group (p < 0.05); however, %LAAI-950 and %LAAE-856 were not significantly different between the two groups (p = 0.864 and 0.956, respectively). The values of airway measurements of Pi10 in the ever-smoker group were 4.29 ± 0.29 mm on inspiratory QCT and 4.29 ± 0.28 mm on expiratory QCT, and both were significantly greater in the ever-smoker group than in the never-smoker group (p < 0.05) (Fig. 1). TLCCT and FRCCT were greater in the ever-smoker group than in the never-smoker group (p < 0.001). Skewness and kurtosis as measured by QCT were not significantly different between the two groups (p > 0.05).

    Fig. 1
    Relationship between inner perimeter (Pi) and square root of wall area (WA) on inspiratory quantitative CT.
    A. 46-year-female never-smoker. B. 53-year-old man who had 30 pack year history. Slope of straight relationship between Pi and square root of WA is calculated and values of square root of WA at designated Pi10 between never-smoker and heavy smoker are 3.75 and 4.34, respectively. Pi10 = Pi of 10 mm

    Table 3
    Comparison between Never-Smoker and Ever-Smoker Group Regarding QCT and PFT

    Comparisons of QCT and PFT Measurements According to Smoking Intensity

    The mean number of smoking pack-years in the ever-smoker group was 22.2 ± 15.1 years. Table 4 shows comparisons of the QCT and PFT measurements according to smoking intensity between the two subgroups divided by mean smoking pack years (cutoff, 20 years) among ever-smokers. All lung attenuation parameters, airway parameters, TLCCT, and FRCCT were not significantly different according to smoking intensity (all p > 0.05).

    Table 4
    Comparisons of QCT and PFT Measurements Grouped by Smoking Intensity

    Comparisons of QCT and PFT Measurements According to Age

    Table 5 shows the results of QCT and PFT measurements according to the age range in all subjects. With an advancement in age, there was tendency towards airflow limitation, and FEV1 (% predicted), FVC (% predicted), and FEV1/FVC were significantly different among the three age groups (p < 0.001). %LAAE-856 gradually increased significantly. However, Pi10 did not change significantly with age. TLCCT gradually decreased as the age increased (p < 0.05).

    Table 5
    Comparisons of QCT and PFT Measurements Grouped by Age

    Correlation of QCT and PFT Measurements after Adjustment for Age and Smoking Intensity

    According to the partial correlation coefficient analysis after adjustment for age and smoking intensity, only Pi10 on inspiration was significantly correlated with FEV1/FVC (r = −0.452, p = 0.012) in all subjects and with FEV1/FVC (r = −0.455, p = 0.003) in ever-smokers. However, Pi10 on inspiration in never-smokers did not correlate with FEV1/FVC (Table 6).

    Table 6
    Correlation between FEV1/FVC and QCT Parameters

    Independent Predictive Variables for Lung Attenuation and Airway Parameters

    In simple and multivariate regression analyses, TLCCT and age, and FRCCT and age showed a significant relationship with LAAI-950 and LAAE-856, respectively, and they were independent predictive variables for lung attenuation (all p < 0.05); however, there were no association with history of smoking or sex groups (p > 0.05) (Table 7). Although inspiratory Pi10 showed a significant relationship with TLCCT, sex, and history of smoking in the simple linear regression model, only TLCCT was significantly associated with inspiratory Pi10 in multiple regression analysis (p = 0.001). None of these parameters were associated with expiratory Pi10 in multiple regression analysis (all p > 0.05).

    Table 7
    Simple and Multiple Regression Analyses for Lung Attenuation and Airway Parameters

    DISCUSSION

    This study provides normal values for QCT parameters of the lungs and airways in Korean never-smokers and ever-smokers with normal spirometry and visually normal chest CT results. There were differences in lung attenuation values and airway parameters according to sex, smoking history, and age; however, there were no differences in smoking intensity among normal subjects. Among the QCT parameters, only inspiratory Pi10 showed a negative correlation with the FEV1/FVC ratio in normal subjects. Furthermore, only status of lung volume and age showed significant associations with inspiratory and expiratory lung attenuation. Also, lung volume itself influenced inspiratory Pi10, whereas expiratory Pi10 was not associated with lung volume, age, sex, or smoking history.

    In a previous research regarding QCT of the lungs and airways in healthy nonsmoking adults, sex differences in the values of inspiratory TLCCT, expiratory FRCCT, and MLA were similar to the present results (10). The previously reported mean %LAAI-950 and %LAAE-856 were 2.0 and 9.2%, respectively, and significant differences were observed according to gender. Although %LAAE-856 (17.9% in men and 15.9% in women) in our study was much greater than that in the previous study, %LAAI-950 values in men (1.78%) and women (1.10%) were compatible to those found in the previous study, and there were no significant sex differences in %LAAI-950 and %LAAE-856. However, we found a significant difference in the HU 15th percentile between males (-908 HU) and females (-891 HU). Indeed, even with the inclusion of ever-smokers in our study and even only in never-smokers, there were no significant sex differences in %LAAI-950 and %LAAE-856, but the HU 15th percentile in men was significantly lower than that in women. This finding differs from the results of the previous study. In normal Koreans, we should consider the sex difference in HU 15th percentile in addition to MLA.

    With respect to the airway parameters of inspiratory and expiratory Pi10 in normal subjects, these values were not mentioned in the previous study (10). In our study, we tried to suggest the absolute value of Pi10 in Korean normal subjects and our results suggest that the Pi10 value in normal Korean male subjects is significantly greater than that in normal Korean female subjects.

    On comparing QCT parameters between the never-smoker and ever-smoker groups, it was found that the values of MLA and HU 15th percentile in the ever-smoker group were significantly lower than those in the never-smoker group. Inspiratory and expiratory Pi10 values were also significantly greater in the ever-smoker group than in the never-smoker group. However, %LAAI-950 and %LAAE-856 were not different between the two groups. We speculate that, even in normal subjects with normal spirometry and visually normal chest CT results, smoking influences lung attenuation and airway remodeling. Furthermore, interestingly, although smoking history itself was found to affect lung attenuation and airway remodeling, smoking intensity did not result in any differences in these parameters. We speculate that smoking itself rather than smoking intensity has a greater influence on lung attenuation and airway remodeling in QCT measurements of normal subjects.

    In our normal subjects, MLAexp was significantly lower and %LAAE-856 was significantly higher as the subjects got older, whereas MLAins and %LAAI-950 were not significantly different according to age. These results are similar to those reported previously (10). In normal subjects, expiratory lung attenuation may be a useful tool for monitoring the normal aging process in the lung tissue. However, Pi10 did not show any significant change according to age.

    In our regression analyses, change in lung attenuation measurements on inspiration and expiration were dependent on age and on TLCCT and FRCCT. These findings are similar to those of previous studies, which showed up to a 1% increase per year in inspiratory %LAA and a significant correlation with age (12, 22, 23). However, in airway measurements obtained in our study, TLCCT showed a strong association with inspiratory Pi10 and it showed no association with expiratory Pi10. This finding may support the notion that the depth or degree of inspiration affects the measurement of airway walls; therefore, expiratory Pi10 is a good independent measure for airway assessment. Moreover, in our study, Pi10 showed a significant correlation with FEV1/FVC.

    Our study has some limitations. First, we did not consider the patients' physical size during CT scans performed using fixed mAs, which may have produced different noise levels during QCT measurements. Second, although there was a significant gender difference in PFT results and QCT measurements between males and females among all subjects and even only in never-smokers, we included a lesser number of ever-smokers (n = 45) than never-smokers (n = 119) and a much higher proportion of male smokers, which may have produced errors while evaluating the influence of smoking in a large cohort. However, we attempted to measure and compare the QCT parameters of the lungs and airways and to demonstrate the effect of smoking in Korean subjects with normal spirometry and visually normal chest CT scans. Third, inter- and intra-CT scanner variation is also a major factor that could have introduced QCT measurement error.

    In this study, in Korean subjects with normal spirometry and visually normal chest CT results, there were significant differences in QCT parameters according to sex, age, and smoking history. The most influential factors for QCT measurements were age and status of lung volume.

    Notes

    This study was supported by the Dongkook Pharm. Co., Ltd., Korea.

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    MeSH Terms
    Tomography, X-Ray Computed*
    Adult
    Aged
    Female
    Forced Expiratory Volume/physiology
    Humans
    Lung/diagnostic imaging*
    Male
    Middle Aged
    Respiratory Function Tests
    Retrospective Studies
    Smoking
    Metrics
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