Studies on isosporosis in dogs. I: Isolation and sporulation of <i>Isospora ohioensis</i>

, Baek, B K; , Kim, C S; , Kim, J H; , Han, K S; , Kim, Y G

doi:1993.31.3.201

Korean J Parasito > Volume 31(3):1993 > Article

Original Article


Korean J Parasitol. 1993 Sep;31(3):201-206. English. Published online Mar 20, 1994. http://dx.doi.org/10.3347/kjp.1993.31.3.201
Copyright © 1993 by The Korean Society for Parasitology


Studies on isosporosis in dogs. I: Isolation and sporulation of Isospora ohioensis
B K Baek,^*¹C S Kim,¹J H Kim,¹K S Han,² and Y G Kim²
¹Chonbuk National University, Chonju, Korea.

Received July 02, 1993; Accepted July 28, 1993.
Abstract
Based on the morphologic characteristics of oocysts of the genus Isospora, we have demonstrated that I. ohioensis is a relatively common Isospora species in dogs which are resident in Chonbuk province, Korea. The prepatent period of I. ohioensis was four days. The size of the unsporulated oocysts in fresh stool specimens was 22.9 × 19.8 µm (R = 1.16). The size of the contracted sporonts was 17.4 × 16.3 µm (R = 1.06). By 96 hours, sporulation is complete and the ratio of length/width was constant relatively. And the sizes of oocysts and sporocysts were 22.8 × 20.5 µm (R = 1.11) and 15.0 × 10.8 µm (R = 1.39), respectively. At this time it is most reliable for the measurements of oocyst sizes of I. ohioensis to provide with the identifiable clues against the others. It is therefore recommended that the clinical fecal specimens suspected of isosporosis should first be incubated and aerated for 96 hours before a definitive parasitological diagnosis can be reached. These and other observations contribute to the understanding of the biological characteristics and laboratory and clinical diagnosis of isosporosis.

Figures


	Fig. 1 The changes of the oocyst sizes of I. ohioensis according to sporulation.


	Fig. 2 The changes of the sporont and sporocyst sizes of I. ohioensis according to sporulation.


	Fig. 3 The length/width ratio of the oocysts, sporonts and sporocysts of I. ohioensis according to sporulation.

Figs. 4-13
Fig. 4: Unsporulated oocyst in the sporont stage with hazy odies of I. ohioensis (×1,000). Figs. 5 and 6: Oocyst of I. ohioensis developing a binucleated sporont and the sporoblast (× 1,000). Fig. 7: Oocyst of I. ohioensis containing a sporont in the developing sporoblast (× 1,000). Fig. 8: Oocyst exhibiting with polar of constriction in sporont (× 1,000). Fig. 9: Oocyst containing two binucleated sporoblasts with polar nuclei (× 1,000). Fig. 10: Oocyst contain two elongated sporocysts (× 1,000). Fig. 11: Oocyst with the developing sporozoite in late sporocyst stage (×1,000). Fig. 12: Oocyst in early sporozoite stage. Each sporocyst contains fully formed sporozoites and dispersed granular residuum (× 1,000). Fig. 13: Oocyst in sporozoite stage each containing a hyaline body and a few dispersed granules (× 1,000).

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