- © 2006 Marshfield Clinic
Innate Immunity SNPs are Associated with Risk for Severe Sepsis after Burn Injury
- Robert C. Barber, PhD,
- Ling-Yu E. Chang, BS,
- Brett D. Arnoldo, MD,
- Gary F. Purdue, MD,
- John L. Hunt, MD,
- Jureta W. Horton, PhD and
- Corinne C. Aragaki, PhD
- Robert C. Barber, PhD, Department of Surgery, University of Texas Southwestern Medical Center, Dallas, Texas USA
- Ling-Yu E. Chang, BS, Department of Surgery, University of Texas Southwestern Medical Center, Dallas, Texas USA
- Brett D. Arnoldo, MD, Department of Surgery, University of Texas Southwestern Medical Center, Dallas, Texas USA
- Gary F. Purdue, MD, Department of Surgery, University of Texas Southwestern Medical Center, Dallas, Texas USA
- John L. Hunt, MD, Department of Surgery, University of Texas Southwestern Medical Center, Dallas, Texas USA
- Jureta W. Horton, PhD, Department of Surgery, University of Texas Southwestern Medical Center, Dallas, Texas USA
- Corinne C. Aragaki, PhD, Divisions of Epidemiology and Biostatistics, University of Texas School of Public Health, Houston, Texas USA
- Reprint Requests:
Robert C. Barber, PhD, University of Texas Southwestern Medical Center, Department of Surgery, 5323 Harry Hines Blvd., Dallas, TX 75390-9160, Tel.: 214-648-8043, Fax: 214-648-8420, Email: robert.barber{at}utsouthwestern.edu
Abstract
Objective: To analyze allelic association with clinical outcome in a cohort of burn patients.
Patients: Two hundred twenty-eight individuals with burns ≥15% total body surface area without significant non-burn related trauma who survived >48 hours post-admission were enrolled. One hundred fifty-nine of these patients were analyzed previously.
Methods: Candidate polymorphisms within interleukin-1 beta (IL-1β), interleukin-6 (IL-6), tumor necrosis factor alpha (TNF-α), cellular differentiation marker 14 (CD14) and toll-like receptor 4 (TLR4) were evaluated by logistic regression analysis for association with increased risk for severe sepsis (sepsis plus organ dysfunction or shock).
Results: After adjustment for age, burn size, ethnicity, gender and inhalation injury, alleles at TNF-α (308G, p=0.013), TLR4 (+896G, p=0.027), IL-6 (174C, p=0.040) and CD14 (159C, p=0.047) were significantly associated with an increased risk for severe sepsis.
Conclusions: Carriage of variant alleles at immune response genes were associated with increased risk for severe sepsis after burn injury.
