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Regenerative Medicine

Immunohistochemical expression of oncological proliferation markers in the hearts of rats during normal pregnancy

    Michele M Ciulla

    * Author for correspondence

    Department of Clinical Science & Community Health, Laboratory of Clinical Informatics & Cardiovascular Imaging, University of Milan, 20122 Milan, Italy.

    ,
    Giulia Acquistapace

    Department of Clinical Science & Community Health, Laboratory of Clinical Informatics & Cardiovascular Imaging, University of Milan, 20122 Milan, Italy

    ,
    Gianluca L Perrucci

    Department of Clinical Science & Community Health, Laboratory of Clinical Informatics & Cardiovascular Imaging, University of Milan, 20122 Milan, Italy

    ,
    Paola Nicolini

    Department of Clinical Science & Community Health, Laboratory of Clinical Informatics & Cardiovascular Imaging, University of Milan, 20122 Milan, Italy

    ,
    Laura Toffetti

    Department of Clinical Science & Community Health, Laboratory of Clinical Informatics & Cardiovascular Imaging, University of Milan, 20122 Milan, Italy

    ,
    Paola Braidotti

    Department of Health Sciences, University of Milan, 20122 Milan, Italy

    ,
    Stefano Ferrero

    Department of Biomedical, Surgical & Dental Science, University of Milan, 20122 Milan, Italy

    Fondazione IRCCS Ca’ Granda Ospedale Maggiore Policlinico, 20122 Milan, Italy

    ,
    Ileana Zucca

    Scientific Direction Unit, Foundation IRCCS Neurological Institute ‘Carlo Besta’, 20133 Milan, Italy

    ,
    Domenico Aquino

    Scientific Direction Unit, Foundation IRCCS Neurological Institute ‘Carlo Besta’, 20133 Milan, Italy

    Neuroradiology Unit, Foundation IRCCS Neurological Institute ‘Carlo Besta’, 20133 Milan, Italy

    ,
    Giuseppe Busca

    Department of Clinical Science & Community Health, Laboratory of Clinical Informatics & Cardiovascular Imaging, University of Milan, 20122 Milan, Italy

    &
    Fabio Magrini

    Department of Clinical Science & Community Health, Laboratory of Clinical Informatics & Cardiovascular Imaging, University of Milan, 20122 Milan, Italy

    Fondazione IRCCS Ca’ Granda Ospedale Maggiore Policlinico, 20122 Milan, Italy

    Published Online:https://doi.org/10.2217/bmm.12.94

    Aim: Pregnancy is characterized by left ventricular hypertrophy that is potentially accounted for by cardiomyocyte proliferation, although no such evidence is currently available. This study investigates if the left ventricular mass (LVM) increase during pregnancy implies cell hyperplasia. Materials & methods: In nonpregnant and late-pregnant rats, cardiac function and LVM were evaluated by MRI, and cardiomyocyte dimensions and proliferations were assessed quantitatively by morphometric analysis and immunohistochemistry using oncological markers (Ki67 and MCM2). Results: In late-pregnant rats, LVM and cardiomyocyte area were greater. No mitotic figures were found nor was there any significant difference between groups in Ki67 expression. MCM2 expression was related to LVM. Conclusion: During pregnancy, rat cardiomyocytes undergo hypertrophy but not hyperplasia; the expression of MCM2, related to LVM, suggests it could be a marker of protein synthesis. The application of oncological markers to physiological contexts may provide insight into their role within the cell cycle.

    Papers of special note have been highlighted as: ▪ of interest ▪▪ of considerable interest

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