Review

Department of Pathology, University of Utah Medical Center, 5B418, 50 North Medical Drive, Salt Lake City, UT 84132, USA.

Email the corresponding author at carl.wittwer@path.utah.edu

Jana O Kent

Department of Pathology, University of Utah Medical Center, 5B418, 50 North Medical Drive, Salt Lake City, UT 84132, USA.

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Email the corresponding author at carl.wittwer@path.utah.edu

Carl T Wittwer

† Author for correspondence

Department of Pathology, University of Utah Medical Center, 5B418, 50 North Medical Drive, Salt Lake City, UT 84132, USA.

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Email the corresponding author at carl.wittwer@path.utah.edu

Published Online:11 Jun 2007https://doi.org/10.2217/14622416.8.6.597

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Abstract

High-resolution melting of DNA is a simple solution for genotyping, mutation scanning and sequence matching. The melting profile of a PCR product depends on its GC content, length, sequence and heterozygosity and is best monitored with saturating dyes that fluoresce in the presence of double-stranded DNA. Genotyping of most variants is possible by the melting temperature of the PCR products, while all variants can be genotyped with unlabeled probes. Mutation scanning and sequence matching depend on sequence differences that result in heteroduplexes that change the shape of the melting curve. High-resolution DNA melting has several advantages over other genotyping and scanning methods, including an inexpensive closed tube format that is homogenous, accurate and rapid. Owing to its simplicity and speed, the method is a good fit for personalized medicine as a rapid, inexpensive method to predict therapeutic response.

Keywords:

Papers of special note have been highlighted as either of interest (•) or of considerable interest (••) to readers.

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- Meloidogyne enterolobii, a Major Threat to Tomato Production: Current Status and Future Prospects for Its Management
  16 November 2020 | Frontiers in Plant Science, Vol. 11
- A microfluidic chip for rapid analysis of DNA melting curves for BRCA2 mutation screening
  1 January 2020 | Lab on a Chip, Vol. 20, No. 20
- One Health Approach to Leishmaniases: Understanding the Disease Dynamics through Diagnostic Tools
  1 October 2020 | Pathogens, Vol. 9, No. 10
- Molecular identification of Candida isolates by Real‐time PCR‐high‐resolution melting analysis and investigation of the genetic diversity of Candida species
  12 July 2020 | Journal of Clinical Laboratory Analysis, Vol. 34, No. 10
- High Resolution Melting and Insertion Site-Based Polymorphism Markers for Wheat Variability Analysis and Candidate Genes Selection at Drought and Heat MQTL Loci
  1 September 2020 | Agronomy, Vol. 10, No. 9
- Development and evaluation of 4 loop-mediated isothermal amplification assays to detect mastitis-causing bacteria in bovine milk samples
  Journal of Dairy Science, Vol. 103, No. 9
- Detection of HBB:c.92+5G>C and HBB:c.108delC mutations in β-thalassemia carriers using high-resolution melting analysis
  7 July 2020 | Molecular Biology Reports, Vol. 47, No. 7
- Development of high-throughput real-time PCR assays for the Colletotrichum acutatum detection on infected olive fruits and olive oils
  Food Chemistry, Vol. 317
- Rapid identification of the Candida glabrata species complex by high‐resolution melting curve analysis
  11 February 2020 | Journal of Clinical Laboratory Analysis, Vol. 34, No. 6
- Rapid screening of MMACHC gene mutations by high‐resolution melting curve analysis
  21 March 2020 | Molecular Genetics & Genomic Medicine, Vol. 8, No. 6
- Use of Machine Learning with Temporal Photoluminescence Signals from CdTe Quantum Dots for Temperature Measurement in Microfluidic Devices
  9 April 2020 | ACS Applied Nano Materials, Vol. 3, No. 5
- High-resolution melting curve analysis: a novel method for identification of Mycoplasma species isolated from clinical cases of bovine and porcine respiratory disease
  1 November 2019 | Tropical Animal Health and Production, Vol. 52, No. 3
- Application of real-time PCR for tree nut allergen detection in processed foods
  13 January 2019 | Critical Reviews in Food Science and Nutrition, Vol. 60, No. 7
- A fast sex detection method for the Whiteleg shrimp Litopenaeus vannamei by post-PCR high resolution melting (HRM)
  Aquaculture, Vol. 520
- Diferenciación morfofisiológica y molecular por curvas de melting de alta resolución (HRMA) y secuenciación del complejo Trichophyton mentagrophytes en cepas humanas en Valparaíso, Chile
  Revista Argentina de Microbiología, Vol. 52, No. 2
- Rapid high resolution melting assay to differentiate Streptococcus suis serotypes 2, 1/2, 1, and 14
  22 January 2020 | MicrobiologyOpen, Vol. 9, No. 4
- Effective Characterization of DNA Ligation Kinetics by High‐Resolution Melting Analysis
  5 December 2019 | ChemBioChem, Vol. 21, No. 6
- Development of a Linker-Mediated Immunoassay Using Chemically Transitioned Nanosensors
  7 February 2020 | Analytical Chemistry, Vol. 92, No. 5
- Mutation spectrum of COL1A1/COL1A2 screening by high-resolution melting analysis of Chinese patients with osteogenesis imperfecta
  14 August 2019 | Journal of Bone and Mineral Metabolism, Vol. 38, No. 2
- Mitochondrial DNA screening by melting curve analysis using peptide nucleic acid probes
  Forensic Science International: Genetics, Vol. 45
- Diagnosis of genetic amyloidosis through the analysis of transthyretin gene mutation using high-resolution melting
  International Journal of Cardiology, Vol. 301
- A High Resolution Melting Analysis-Based Genotyping Toolkit for the Peach (Prunus persica) Chilling Requirement
  24 February 2020 | International Journal of Molecular Sciences, Vol. 21, No. 4
- Streptavidin Homologues for Applications on Solid Surfaces at High Temperatures
  2 January 2020 | Langmuir, Vol. 36, No. 2
- Optimization of High Resolution Melting (HRM) Method for Genotyping and Estimating Frequency of SNP rs4284505 in the Vietnamese Breast Cancer
  6 June 2019
- High-resolution melt analysis enables simple genotyping of complicated polymorphisms of codon 18 rendering the NUDT15 diplotype
  22 October 2019 | Journal of Gastroenterology, Vol. 55, No. 1
- Identification and differentiation of Campylobacter isolated from chicken meat using real-time polymerase chain reaction and high resolution melting analysis of hipO and glyA genes
  15 September 2020 | Veterinary World, Vol. 13, No. 9
- Development of the Genus and Species Determination Method for Histamine Producing Bacteria Isolated from Fishery Product with High-Resolution Melting Analysis
  Food Hygiene and Safety Science (Shokuhin Eiseigaku Zasshi), Vol. 60, No. 6
- Comparison Between Three Molecular Diagnostics for the Identification of Heterozygous Hemoglobin E
  Pakistan Journal of Biological Sciences, Vol. 23, No. 1
- Distinguishing Anuran species by high‐resolution melting analysis of the COI barcode (COI‐HRM)
  6 November 2019 | Ecology and Evolution, Vol. 9, No. 23
- Immunohistochemistry as a Genetic Surrogate in Dermatopathology: Pearls and Pitfalls
  20 September 2019 | Advances in Anatomic Pathology, Vol. 26, No. 6
- A Statistical Method to Enhance the Analysis of the Differences Among High‐Resolution Melting (HRM) Curves of PCR‐Amplified DNA Fragments
  3 October 2019 | Journal of Food Science, Vol. 84, No. 10
- Evaluation of High-Resolution Melting for Rapid Differentiation of Phytophthora Hybrids and Their Parental Species
  Plant Disease, Vol. 103, No. 9
- DNA Mini-Barcoding: A Derived Barcoding Method for Herbal Molecular Identification
  28 August 2019 | Frontiers in Plant Science, Vol. 10
- Detection of Golovinomyces orontii using species-specific primers and high-resolution melting analysis
  29 May 2019 | Tropical Plant Pathology, Vol. 44, No. 4
- Comparison of molecular genetic methods of detection of mutations in the CALR gene in myeloproliferative disorders
  27 July 2019 | Advances in molecular oncology, Vol. 6, No. 2
- RETRACTED: Point mutation detection by economic HRM protocol primer design
  Biochemistry and Biophysics Reports, Vol. 18
- Emerging Analytical Techniques for Rapid Pathogen Identification and Susceptibility Testing
  Annual Review of Analytical Chemistry, Vol. 12, No. 1
- Genotyping of a 50 bp insertion/deletion genetic variation at promoter region of the superoxide dismutase 1 (SOD1) using high resolution melting analysis
  Gene Reports, Vol. 15
- Coinheritance of Hb A 2 -Melbourne ( HBD : c.130G>A) and Hb E ( HBB : c.79G>A) in Laos and Simultaneous High Resolution Melt Detection of Hb A 2 -Melbourne and Hb A 2 -Lampang ( HBD : c.142G>A) in a Single Tube
  26 August 2019 | Hemoglobin, Vol. 43, No. 3
- A ‘culture’ shift: Application of molecular techniques for diagnosing polymicrobial infections
  Biotechnology Advances, Vol. 37, No. 3
- SNP development and diversity analysis for Ginkgo biloba based on transcriptome sequencing
  2 January 2019 | Trees, Vol. 33, No. 2
- Application of high-resolution melting curve analysis for identification of Muscovy duck parvovirus and goose parvovirus
  Journal of Virological Methods, Vol. 266
- High-resolution melt (HRM) analysis for detection of SNPs associated with pyrethroid resistance in the southern cattle fever tick, Rhipicephalus (Boophilus) microplus (Acari: Ixodidae)
  International Journal for Parasitology: Drugs and Drug Resistance, Vol. 9
- High-resolution melting analysis for testing the authenticity of fresh and canned heart of palm, an economically important non-timber forest product from the Neotropics
  13 April 2019 | Plant Ecology & Diversity, Vol. 12, No. 2
- Molecular basis of familial adenomatous polyposis in the southeast of Brazil: identification of six novel mutations
  11 March 2019 | The International Journal of Biological Markers, Vol. 34, No. 1
- Development and validation of a rapid method for genotyping three P‐selectin gene polymorphisms based on high resolution melting analysis
  23 October 2018 | Journal of Clinical Laboratory Analysis, Vol. 33, No. 3
- Food Genomics for the Characterization of PDO and PGI Virgin Olive Oils
  7 December 2018 | European Journal of Lipid Science and Technology, Vol. 121, No. 3
- Application of real-time PCR (qPCR) for characterization of microbial populations and type of milk in dairy food products
  4 October 2017 | Critical Reviews in Food Science and Nutrition, Vol. 59, No. 3
- Combining magnetic nanoparticle capture and poly-enzyme nanobead amplification for ultrasensitive detection and discrimination of DNA single nucleotide polymorphisms
  1 January 2019 | Nanoscale, Vol. 11, No. 3
- Real-time PCR followed by high-resolution melting analysis – a new robust approach to evaluate SCCmec typing of methicillin-resistant Staphylococcus aureus
  Yasaman Sadeghi,
  Seyed Alireza Salami,
  Pegah Kananizadeh,
  Sayed-Hamidreza Mozhgani &
  Mohammad Reza Pourmand
  22 January 2019 | Future Microbiology, Vol. 14, No. 2
- Real-time PCR applications for diagnosis of leishmaniasis
  2 May 2018 | Parasites & Vectors, Vol. 11, No. 1
- Discrimination between some Mycoplasma spp. and Acholeplasma laidlawii in bovine milk using high resolution melting curve analysis
  7 February 2018 | BMC Research Notes, Vol. 11, No. 1
- Development of High-Throughput SNP Genotyping Assays for Rapid Detection of Strawberry Colletotrichum Species and the G143A Mutation
  Phytopathology®, Vol. 108, No. 12
- Molecular bloodmeal analyses reveal that Trypanosoma cruzi ‐infected, native triatomine bugs often feed on humans in houses in central Brazil
  12 July 2018 | Medical and Veterinary Entomology, Vol. 32, No. 4
- Validation of high-resolution melting analysis as a diagnostic tool for endothelin receptor B mutation in American Paint horses and allele frequency estimation
  Molecular and Cellular Probes, Vol. 41
- A “Culture” Shift: Broad Bacterial Detection, Identification, and Antimicrobial Susceptibility Testing Directly from Whole Blood
  1 October 2018 | Clinical Chemistry, Vol. 64, No. 10
- DNA Melting Analysis with Optofluidic Lasers Based on Fabry-Pérot Microcavity
  24 August 2018 | ACS Sensors, Vol. 3, No. 9
- FDM 3D Printing of High-Pressure, Heat-Resistant, Transparent Microfluidic Devices
  3 August 2018 | Analytical Chemistry, Vol. 90, No. 17
- The applicability of real-time PCR in the diagnostic of cutaneous leishmaniasis and parasite quantification for clinical management: Current status and perspectives
  Acta Tropica, Vol. 184
- High molecular prevalence of Leishmania in phlebotomine sand flies fed on chicken blood in Brazil
  Veterinary Parasitology, Vol. 259
- The potential of aerosol eDNA sampling for the characterisation of commercial seed lots
  1 August 2018 | PLOS ONE, Vol. 13, No. 8
- High-resolution melting-based TILLING of γ ray-induced mutations in rice
  11 August 2018 | Journal of Zhejiang University-SCIENCE B, Vol. 19, No. 8
- An integrated genetic map based on EST-SNPs and QTL analysis of shell color traits in Pacific oyster Crassostrea gigas
  Aquaculture, Vol. 492
- High resolution melting analysis (HRM) for the assessment of clonality in feline B-cell lymphomas
  Veterinary Immunology and Immunopathology, Vol. 200
- Detection of the Trp-2027-Cys Mutation in Fluazifop-P-butyl–resistant Itchgrass ( Rottboellia cochinchinensis ) using High-Resolution Melting Analysis (HRMA)
  2 April 2018 | Weed Science, Vol. 66, No. 3
- New rapid PCR protocol based on high‐resolution melting analysis to identify Saccharomyces cerevisiae and other species within its genus
  23 February 2018 | Journal of Applied Microbiology, Vol. 124, No. 5
- Candidate DNA Barcode Tags Combined With High Resolution Melting (Bar-HRM) Curve Analysis for Authentication of Senna alexandrina Mill. With Validation in Crude Drugs
  13 March 2018 | Frontiers in Plant Science, Vol. 9
- Characterisation of ail-positive Yersinia enterocolitica of different biotypes using HRMA
  International Journal of Food Microbiology, Vol. 269
- Evaluation of Two Multiplex PCR–High-Resolution Melt Curve Analysis Methods for Differentiation of Campylobacter jejuni and Campylobacter coli Intraspecies
  Avian Diseases, Vol. 62, No. 1
- GJA4/Connexin 37 Mutations Correlate with Secondary Lymphedema Following Surgery in Breast Cancer Patients
  22 February 2018 | Biomedicines, Vol. 6, No. 1
- Novel diagnostic tools for Asian (Apis cerana) and European (Apis mellifera) honey authentication
  Food Research International, Vol. 105
- Development of a triplex real-time PCR for simultaneous detection of allergenic ingredients in processed food
  Czech Journal of Food Sciences, Vol. 36, No. 1
- Mutation Testing of Lung Cancer Biomarkers (Excluding IHC and NGS)
  14 August 2017
- Method for Identifying HbCS Variant in Alpha Globin Genes Causing Alpha-Thalassaemia Disease
  24 September 2017
- Primary Study of SNP rs2046210 in Vietnamese Breast Cancer Population by High-Resolution Melting Analysis (HRMA)
  24 September 2017
- Applying high-resolution melting (HRM) technology to olive oil and wine authenticity
  Food Research International, Vol. 103
- Tm -Shift Detection of Dog-Derived Ancylostoma ceylanicum and A. caninum
  BioMed Research International, Vol. 2018
- Molecular testing for BRAF mutations to inform melanoma treatment decisions: a move toward precision medicine
  Modern Pathology, Vol. 31, No. 1
- Development of DNA markers to differentiate good Kyokai sake yeast and other yeast strains
  JOURNAL OF THE BREWING SOCIETY OF JAPAN, Vol. 113, No. 10
- Species identification in meat products: A new screening method based on high resolution melting analysis of cyt b gene
  Food Chemistry, Vol. 237
- Reproductive interference and fecundity affect competitive interactions of sibling species with low mating barriers: experimental and theoretical evidence
  13 September 2017 | Heredity, Vol. 119, No. 6
- Discrimination of three genetically close Aspergillus species by using high resolution melting analysis applied to indoor air as case study
  4 April 2017 | BMC Microbiology, Vol. 17, No. 1
- High resolution melting: a useful field-deployable method to measure dhfr and dhps drug resistance in both highly and lowly endemic Plasmodium populations
  19 April 2017 | Malaria Journal, Vol. 16, No. 1
- Fast and Reliable Differentiation of Eight Trichinella Species Using a High Resolution Melting Assay
  24 November 2017 | Scientific Reports, Vol. 7, No. 1
- Rapid discrimination of Isaria javanica and Isaria poprawskii from Isaria spp. using high resolution DNA melting assays
  Journal of Invertebrate Pathology, Vol. 150
- Characterization of novel EST-SNP markers and their association analysis with growth-related traits in the Pacific oyster Crassostrea gigas
  12 April 2017 | Aquaculture International, Vol. 25, No. 5
- Diagnóstico molecular de cavernomatosis cerebral
  Neurología, Vol. 32, No. 8
- Molecular diagnosis in cerebral cavernous malformations
  Neurología (English Edition), Vol. 32, No. 8
- HRM and SNaPshot as alternative forensic SNP genotyping methods
  18 May 2017 | Forensic Science, Medicine and Pathology, Vol. 13, No. 3
- High-resolution melting analysis of gyrA codon 84 and grlA codon 80 mutations conferring resistance to fluoroquinolones in Staphylococcus pseudintermedius isolates from canine clinical samples
  5 June 2017 | Journal of Veterinary Diagnostic Investigation, Vol. 29, No. 5
- Modern analytical methods for the detection of food fraud and adulteration by food category
  24 May 2017 | Journal of the Science of Food and Agriculture, Vol. 97, No. 12
- Rapid identification of the north-western Pacific billfish species using quantitative real-time polymerase chain reaction techniques
  22 May 2017 | Marine Biology Research, Vol. 13, No. 7
- Genotype-specific real-time PCR combined with high-resolution melting analysis for rapid identification of red-spotted grouper nervous necrosis virus
  27 April 2017 | Archives of Virology, Vol. 162, No. 8
- qPCR and HRM-based diagnosis of SNPs on growth differentiation factor 9 (GDF9), a gene associated with sheep (Ovis aries) prolificacy
  30 June 2017 | 3 Biotech, Vol. 7, No. 3
- High‐resolution melt analysis without DNA extraction affords rapid genotype resolution and species identification
  18 October 2016 | Molecular Ecology Resources, Vol. 17, No. 4
- ArtinM Binding Effinities and Kinetic Interaction with Leukemia Cells: A Quartz Crystal Microbalance Bioelectroanalysis on the Cytotoxic Effect
  8 March 2017 | Electroanalysis, Vol. 29, No. 6
- LNA Thymidine Monomer Enables Differentiation of the Four Single-Nucleotide Variants by Melting Temperature
  9 May 2017 | Journal of the American Chemical Society, Vol. 139, No. 20
- Evaluation of PCR and high-resolution melt curve analysis for differentiation of Salmonella isolates
  15 February 2017 | Avian Pathology, Vol. 46, No. 3
- Study of Commercially Available Lobelia chinensis Products Using Bar-HRM Technology
  16 March 2017 | Frontiers in Plant Science, Vol. 8
- COLD-HRM: A Combination of Methods to Infer the Nature of Somatic Mutations
  7 March 2017 | Advances in Cytology & Pathology, Vol. 2, No. 2
- Dynamic time warping assessment of high-resolution melt curves provides a robust metric for fungal identification
  6 March 2017 | PLOS ONE, Vol. 12, No. 3
- Molecular markers for improving control of soil-borne pathogen Fusarium oxysporum in sugar beet
  18 February 2017 | Euphytica, Vol. 213, No. 3
- TILLING, high-resolution melting (HRM), and next-generation sequencing (NGS) techniques in plant mutation breeding
  11 March 2017 | Molecular Breeding, Vol. 37, No. 3
- Blending DNA binding dyes to improve detection in real-time PCR
  Biotechnology Reports, Vol. 14
- High-resolution melting (HRM) for genotyping bovine ephemeral fever virus (BEFV)
  Virus Research, Vol. 229
- High-resolution melting analysis for rapid and sensitive NOTCH1 screening in chronic lymphocytic leukemia
  5 January 2017 | International Journal of Molecular Medicine, Vol. 39, No. 2
- Forensically relevant SNaPshot® assays for human DNA SNP analysis: a review
  14 November 2016 | International Journal of Legal Medicine, Vol. 131, No. 1
- High-Resolution Melting Curve Analysis for Molecular Diagnostics
- Study of the role of tumor necrosis factor-α (–308 G/A) and interleukin-10 (–1082 G/A) polymorphisms as potential risk factors to acute kidney injury in patients with severe sepsis using high-resolution melting curve analysis
  27 October 2016 | Renal Failure, Vol. 39, No. 1
- Rapid screening for targeted genetic variants via high-resolution melting curve analysis
  Clinical Chemistry and Laboratory Medicine (CCLM), Vol. 55, No. 4
- High-resolution melting analysis for detection of a single-nucleotide polymorphism and the genotype of the myostatin gene in warmblood horses
  American Journal of Veterinary Research, Vol. 78, No. 1
- Molecular Identification of Broomrape Species from a Single Seed by High Resolution Melting Analysis
  12 December 2016 | Frontiers in Plant Science, Vol. 7
- Identification of Ixodes ricinus blood meals using an automated protocol with high resolution melting analysis (HRMA) reveals the importance of domestic dogs as larval tick hosts in Italian alpine forests
  12 December 2016 | Parasites & Vectors, Vol. 9, No. 1
- The source of SYBR green master mix determines outcome of nucleic acid amplification reactions
  4 June 2016 | BMC Research Notes, Vol. 9, No. 1
- Molecular typing of bacteria for epidemiological surveillance and outbreak investigation / Molekulare Typisierung von Bakterien für die epidemiologische Überwachung und Ausbruchsabklärung
  30 December 2016 | Die Bodenkultur: Journal of Land Management, Food and Environment, Vol. 67, No. 4
- Advanced DNA- and Protein-based Methods for the Detection and Investigation of Food Allergens
  7 April 2015 | Critical Reviews in Food Science and Nutrition, Vol. 56, No. 15
- Detection of sdhB Gene Mutations in SDHI-Resistant Isolates of Botrytis cinerea Using High Resolution Melting (HRM) Analysis
  15 November 2016 | Frontiers in Microbiology, Vol. 7
- High resolution melting analysis as a new approach to discriminate gluten-containing cereals
  Food Chemistry, Vol. 211
- A comparison of PCR assays for beak and feather disease virus and high resolution melt (HRM) curve analysis of replicase associated protein and capsid genes
  Journal of Virological Methods, Vol. 237
- Development and Validation of High-Resolution Melting Markers Derived from Rysto STS Markers for High-Throughput Marker-Assisted Selection of Potato Carrying Rysto
  Phytopathology®, Vol. 106, No. 11
- The spxB gene as a target to identify Lactobacillus casei group species in cheese
  Food Microbiology, Vol. 59
- Development of a quantitation approach for total human and male DNA based on real time PCR followed by high resolution melting analysis
  7 September 2016 | ELECTROPHORESIS, Vol. 37, No. 21
- A Review of Whole-Exome Sequencing Efforts Toward Hereditary Breast Cancer Susceptibility Gene Discovery
  27 June 2016 | Human Mutation, Vol. 37, No. 9
- Detection of a G>C single nucleotide polymorphism within a repetitive DNA sequence by high-resolution DNA melting
  14 March 2016 | International Journal of Legal Medicine, Vol. 130, No. 5
- Application of high-resolution melting analysis for differentiation of spoilage yeasts
  31 August 2016 | Journal of Microbiology, Vol. 54, No. 9
- Optimizing a multiplex high resolution melting curve to diagnose G6PD deficiency based on viangchan and canton mutations
  28 September 2016 | Biomedical Research and Therapy, Vol. 3, No. 8
- Identification and Differentiation of Monilinia Species Causing Brown Rot of Pome and Stone Fruit using High-Resolution Melting (HRM) Analysis
  Phytopathology®, Vol. 106, No. 9
- High resolution melting curve analysis with MATLAB-based program
  Measurement, Vol. 90
- Evolution and perspectives of cultivar identification and traceability from tree to oil and table olives by means of DNA markers
  15 April 2016 | Journal of the Science of Food and Agriculture, Vol. 96, No. 11
- Wide genetic variations at 18S ribosomal RNA locus of Cyclospora cayetanensis isolated from Egyptian patients using high resolution melting curve
  4 April 2016 | Parasitology Research, Vol. 115, No. 7
- SNP-Based Differentiation of Phytophthora infestans Clonal Lineages Using Locked Nucleic Acid Probes and High-Resolution Melt Analysis
  Plant Disease, Vol. 100, No. 7
- Lineage-specific detection of influenza B virus using real-time polymerase chain reaction with melting curve analysis
  29 February 2016 | Archives of Virology, Vol. 161, No. 6
- Genetic polymorphisms of T-1131C APOA5 and ALOX5AP SG13S114 with the susceptibility of ischaemic stroke in Morocco
  16 May 2016 | Journal of Genetics, Vol. 95, No. 2
- Polymerase chain reaction and chemometrics detected several Pinus species including Pinus armandii involved in pine nut syndrome
  Food Control, Vol. 64
- Association between ADAM12 polymorphism and knee osteoarthritis in Thai population
  The Knee, Vol. 23, No. 3
- High-Resolution DNA Melting Analysis in Plant Research
  Trends in Plant Science, Vol. 21, No. 6
- Comparison of multiple genotyping methods for the identification of the cancer predisposing founder mutation p.R337H in TP53
  3 June 2016 | Genetics and Molecular Biology, Vol. 39, No. 2
- Automated Microfluidic Platform for Serial Polymerase Chain Reaction and High-Resolution Melting Analysis
  SLAS Technology, Vol. 21, No. 3
- A simplified strategy for sensitive detection of Rose rosette virus compatible with three RT-PCR chemistries
  Journal of Virological Methods, Vol. 232
- A Rapid Multiplex Real-Time PCR High-Resolution Melt Curve Assay for the Simultaneous Detection of Bacillus cereus, Listeria monocytogenes, and Staphylococcus aureus in Food
  Journal of Food Protection, Vol. 79, No. 5
- Genotyping-by-sequencing of a bi-parental mapping population segregating for downy mildew resistance in hop (Humulus lupulus L.)
  1 December 2015 | Euphytica, Vol. 208, No. 3
- The use of real-time polymerase chain reaction with high resolution melting (real-time PCR-HRM) analysis for the detection and discrimination of nematodes Bursaphelenchus xylophilus and Bursaphelenchus mucronatus
  Molecular and Cellular Probes, Vol. 30, No. 2
- Mutation scanning of BRAF , NRAS , KIT , and GNAQ / GNA 11 in oral mucosal melanoma: a study of 57 cases
  24 September 2015 | Journal of Oral Pathology & Medicine, Vol. 45, No. 4
- The Potential Power of Bar-HRM Technology in Herbal Medicine Identification
  30 March 2016 | Frontiers in Plant Science, Vol. 7
- Fine-needle aspiration of small pulmonary nodules yields material for reliable molecular analysis of adenocarcinomas
  Journal of the American Society of Cytopathology, Vol. 5, No. 2
- Involvement of CELSR3 Hypermethylation in Primary Oral Squamous Cell Carcinoma
  Asian Pacific Journal of Cancer Prevention, Vol. 17, No. 1
- A novel pentaplex real time (RT)- PCR high resolution melt curve assay for simultaneous detection of emetic and enterotoxin producing Bacillus cereus in food
  Food Control, Vol. 60
- Real-time PCR melting analysis with fiber optic SPR enables multiplex DNA identification of bacteria
  1 January 2016 | The Analyst, Vol. 141, No. 6
- Allelic Frequency of a 24-bp Duplication in Exon 10 of the CHIT1 Gene in the General Iranian Population
  Genetic Testing and Molecular Biomarkers, Vol. 20, No. 1
- Chemical and Molecular Characterization of Crude Oil Obtained by Olive-Pomace Recentrifugation
  Journal of Chemistry, Vol. 2016
- Molecular Methodologies and Molecular Pathology of Hematolymphoid Neoplasms
- Development of High Resolution Melting Analysis for the Diagnosis of Human Malaria
  28 October 2015 | Scientific Reports, Vol. 5, No. 1
- Rapid Detection Method for the Four Most Common CHEK2 Mutations Based on Melting Profile Analysis
  7 October 2015 | Molecular Diagnosis & Therapy, Vol. 19, No. 6
- Optimization of melting analysis with TaqMan probes for detection of KRAS, NRAS, and BRAF mutations
  Analytical Biochemistry, Vol. 491
- Quantitative real-time PCR and high-resolution melting (HRM) analysis for strain-specific monitoring of fluorescent pseudomonads used as biocontrol agents against soil-borne pathogens of food crops
  Trends in Food Science & Technology, Vol. 46, No. 2
- DETECTION AND DIFFERENTIATION OF THE VACCINE STRAIN AND FIELD ISOLATES OF DUCK HEPATITIS A VIRUS TYPE 1 USING REAL-TIME RT-PCR AND HIGH RESOLUTION MELTING ASSAYS
  19 January 2016 | Taiwan Veterinary Journal, Vol. 41, No. 04
- Papillary glioneuronal tumors: histological and molecular characteristics and diagnostic value of SLC44A1-PRKCA fusion
  15 December 2015 | Acta Neuropathologica Communications, Vol. 3, No. 1
- High resolution melting analysis of DNA microsatellites in olive pastes and virgin olive oils obtained by talc addition
  30 March 2015 | European Journal of Lipid Science and Technology, Vol. 117, No. 12
- Generation of Dynamic Free-Form Temperature Gradients in a Disposable Microchip
  2 November 2015 | Analytical Chemistry, Vol. 87, No. 22
- High-Resolution Melting Curve Analysis for Identification of Pasteurellaceae Species in Experimental Animal Facilities
  10 November 2015 | PLOS ONE, Vol. 10, No. 11
- rpoB gene high-resolution melt curve analysis: a rapid approach for diagnosis and screening of drug resistance in tuberculous meningitis
  Diagnostic Microbiology and Infectious Disease, Vol. 83, No. 2
- Differentiation of Campylobacter jejuni and Campylobacter coli Using Multiplex-PCR and High Resolution Melt Curve Analysis
  22 September 2015 | PLOS ONE, Vol. 10, No. 9
- Approaches for identifying multiple-SNP haplotype blocks for use in human identification
  Legal Medicine, Vol. 17, No. 5
- Rapid differentiation of mycobacteria by simplex real-time PCR with melting temperature calling analysis
  2 August 2015 | Journal of Applied Microbiology, Vol. 119, No. 3
- (R)evolution in the Molecular Sexing of Ratite Birds: Identification and Analysis of New Candidate Sex-Linked Markers
  3 September 2015 | Avian Biology Research, Vol. 8, No. 3
- Identifying the last bloodmeal of questing sheep tick nymphs (Ixodes ricinus L.) using high resolution melting analysis
  Veterinary Parasitology, Vol. 210, No. 3-4
- Incidence and molecular characterization of Glucose‐6‐Phosphate Dehydrogenase deficiency among neonates for newborn screening in Chaozhou, China
  1 December 2014 | International Journal of Laboratory Hematology, Vol. 37, No. 3
- Evaluation of the Capacity of PCR and High-Resolution Melt Curve Analysis for Identification of Mixed Infection with Mycoplasma gallisepticum Strains
  13 May 2015 | PLOS ONE, Vol. 10, No. 5
- Mechanism behind Resistance against the Organophosphate Azamethiphos in Salmon Lice (Lepeophtheirus salmonis)
  20 April 2015 | PLOS ONE, Vol. 10, No. 4
- High-Resolution Melting approaches towards plant fungal molecular diagnostics
  15 November 2014 | Phytoparasitica, Vol. 43, No. 2
- Identification of Brucella spp. isolated from human brucellosis in Malaysia using high-resolution melt (HRM) analysis
  Diagnostic Microbiology and Infectious Disease, Vol. 81, No. 4
- Development of a Single Nucleotide Polymorphism Barcode to Genotype Plasmodium vivax Infections
  17 March 2015 | PLOS Neglected Tropical Diseases, Vol. 9, No. 3
- Rapid Identification of Medically Important Candida Isolates Using High Resolution Melting Analysis
  17 February 2015 | PLOS ONE, Vol. 10, No. 2
- High-resolution melting (HRM) analysis: a highly sensitive alternative for the identification of commercially important Crassostrea oysters
  17 October 2014 | Journal of Molluscan Studies, Vol. 81, No. 1
- High resolution melting analytical platform for rapid prenatal and postnatal diagnosis of β-thalassemia common among Southeast Asian population
  Clinica Chimica Acta, Vol. 441
- Applied oilseed rape marker technology and genomics
- Development of a high-resolution melting genotyping assay for the angiotensin I converting enzyme insertion/deletion polymorphism and establishment of genotype-specific reference intervals in a Danish population
  2 April 2014 | Annals of Clinical Biochemistry: International Journal of Laboratory Medicine, Vol. 52, No. 1
- Formalin-Fixed Paraffin-Embedded Tissue (FFPET) Sections for Nucleic Acid-Based Analysis in Biomarker Discovery and Early Drug Development
  19 June 2015
- High-Resolution Melting Analysis for Genotyping Duffy Blood Group Antigens
- High-Resolution Melting of 12S rRNA and Cytochrome b DNA Sequences for Discrimination of Species within Distinct European Animal Families
  22 December 2014 | PLoS ONE, Vol. 9, No. 12
- Competitive amplification of differentially melting amplicons facilitates efficient genotyping of photoperiod- and temperature-sensitive genic male sterility in rice
  4 July 2014 | Molecular Breeding, Vol. 34, No. 4
- Development of a real-time PCR melt curve assay for simultaneous detection of virulent and antibiotic resistant Salmonella
  Food Microbiology, Vol. 44
- G894T endothelial nitric oxide synthase polymorphism and ischemic stroke in Morocco
  Meta Gene, Vol. 2
- An EPAS1 Haplotype Is Associated With High Altitude Polycythemia in Male Han Chinese at the Qinghai-Tibetan Plateau
  Wilderness & Environmental Medicine, Vol. 25, No. 4
- Comparison of high resolution melting analysis, pyrosequencing, next generation sequencing and immunohistochemistry to conventional Sanger sequencing for the detection of p.V600E and non-p.V600E BRAFmutations
  10 January 2014 | BMC Cancer, Vol. 14, No. 1
- DNA fingerprinting in botany: past, present, future
  3 January 2014 | Investigative Genetics, Vol. 5, No. 1
- The use of the HRM method for identifying possible mutations in the ymoA gene region and evaluating their influence on the enterotoxic properties of Y. enterocoliticastrains
  19 September 2014 | BMC Veterinary Research, Vol. 10, No. 1
- Gene therapy approach to FAP: in vivo influence of T119M in TTR deposition in a transgenic V30M mouse model
  2 October 2014 | Gene Therapy, Vol. 21, No. 12
- Molecular pathology in lung cancer: a guide to the techniques used in clinical practice
  30 October 2014 | Histopathology, Vol. 65, No. 6
- Simultaneous Detection of Salmonella, Listeria monocytogenes, and Staphylococcus aureus by Multiplex Real-Time PCR Assays Using High-Resolution Melting
  27 April 2014 | Food Analytical Methods, Vol. 7, No. 10
- Rapid genotyping of beak and feather disease virus using high-resolution DNA melt curve analysis
  Journal of Virological Methods, Vol. 208
- Development of a two-step high-resolution melting (HRM) analysis for screening sequence variants associated with resistance to the QoIs, benzimidazoles and dicarboximides in airborne inoculum of Botrytis cinerea
  22 September 2014 | FEMS Microbiology Letters, Vol. 360, No. 2
- The genotyping of Infectious bronchitis virus in Taiwan by a multiplex amplification refractory mutation system reverse transcription polymerase chain reaction
  15 September 2014 | Journal of Veterinary Diagnostic Investigation, Vol. 26, No. 6
- Melting analysis on microbeads in rapid temperature-gradient inside microchannels for single nucleotide polymorphisms detection
  26 November 2014 | Biomicrofluidics, Vol. 8, No. 6
- Point mutation frequency in the FMR1 gene as revealed by fragile X syndrome screening
  Molecular and Cellular Probes, Vol. 28, No. 5-6
- High resolution melting analysis for the differentiation of Mycobacterium species
  Journal of Medical Microbiology, Vol. 63, No. 10
- Evaluation of high-resolution melting and other molecular methods in discrimination of Lactobacillus isolates
  19 August 2014 | Journal of Applied Microbiology, Vol. 117, No. 4
- SNPs in Genes Functional in Starch-Sugar Interconversion Associate with Natural Variation of Tuber Starch and Sugar Content of Potato ( Solanum tuberosum L.)
  1 October 2014 | G3 Genes|Genomes|Genetics, Vol. 4, No. 10
- Rapid screening of rpoB and katG mutations in Mycobacterium tuberculosis isolates by high-resolution melting curve analysis
  Indian Journal of Medical Microbiology, Vol. 32, No. 4
- Update on Colon Cancer Screening: Recent Advances and Observations in Colorectal Cancer Screening
  10 August 2014 | Current Gastroenterology Reports, Vol. 16, No. 9
- Study on the LAMP-PCR-hybridization–thermal melt–ELISA method for molecular detection of multidrug resistance in Mycobacterium tuberculosis isolates
  Biomarkers and Genomic Medicine, Vol. 6, No. 3
- High resolution melting (HRM) analysis of DNA – Its role and potential in food analysis
  Food Chemistry, Vol. 158
- Simultaneous detection of Cherry necrotic rusty mottle virus and Cherry green ring mottle virus using real-time PCR and high resolution melting analysis
  Molecular and Cellular Probes, Vol. 28, No. 4
- High-resolution melting analysis for mutation scanning in the non-coding control region of JC polyomavirus from patients with progressive multifocal leukoencephalopathy
  25 January 2014 | Archives of Virology, Vol. 159, No. 7
- High‐Resolution Melt as a Screening Method in Autosomal Dominant Polycystic Kidney Disease (ADPKD)
  22 March 2014 | Journal of Clinical Laboratory Analysis, Vol. 28, No. 4
- The application of high resolution melting in the analysis of simple sequence repeat and single nucleotide polymorphism markers in a pea (Pisum sativum L.) population
  Czech Journal of Genetics and Plant Breeding, Vol. 50, No. 2
- Enzymatic Treatment of Specimens before DNA Extraction Directly Influences Molecular Detection of Infectious Agents
  17 June 2014 | PLoS ONE, Vol. 9, No. 6
- Uncovering the Self-Assembly of DNA Nanostructures by Thermodynamics and Kinetics
  22 May 2014 | Accounts of Chemical Research, Vol. 47, No. 6
- A HRM Real-Time PCR Assay for Rapid and Specific Identification of the Emerging Pest Spotted-Wing Drosophila (Drosophila suzukii)
  13 June 2014 | PLoS ONE, Vol. 9, No. 6
- Genotyping Accuracy of High-Resolution DNA Melting Instruments
  1 June 2014 | Clinical Chemistry, Vol. 60, No. 6
- Isocitrate dehydrogenase mutation is associated with tumor location and magnetic resonance imaging characteristics in astrocytic neoplasms
  28 March 2014 | Oncology Letters, Vol. 7, No. 6
- The development and validation of a rapid genetic method for species identification and genotyping of medically important fungal pathogens using high‐resolution melting curve analysis
  12 April 2014 | Molecular Oral Microbiology, Vol. 29, No. 3
- Identification of lactic acid bacteria isolated from poultry carcasses by high-resolution melting (HRM) analysis
  7 January 2014 | European Food Research and Technology, Vol. 238, No. 4
- Summer Squash Identification by High-Resolution-Melting (HRM) Analysis Using Gene-Based EST–SSR Molecular Markers
  26 September 2013 | Plant Molecular Biology Reporter, Vol. 32, No. 2
- Development, inheritance and evaluation of 55 novel single nucleotide polymorphism markers for parentage assignment in the Pacific oyster (Crassostrea gigas)
  16 October 2013 | Genes & Genomics, Vol. 36, No. 2
- Large-Scale Development of Gene-Associated Single-Nucleotide Polymorphism Markers for Molluscan Population Genomic, Comparative Genomic, and Genome-Wide Association Studies
  25 November 2013 | DNA Research, Vol. 21, No. 2
- Reliable Discrimination of 10 Ungulate Species Using High Resolution Melting Analysis of Faecal DNA
  17 March 2014 | PLoS ONE, Vol. 9, No. 3
- Accurate Zygote‐Specific Discrimination of Single‐Nucleotide Polymorphisms Using Microfluidic Electrochemical DNA Melting Curves
  12 February 2014 | Angewandte Chemie International Edition, Vol. 53, No. 12
- Accurate Zygote‐Specific Discrimination of Single‐Nucleotide Polymorphisms Using Microfluidic Electrochemical DNA Melting Curves
  12 February 2014 | Angewandte Chemie, Vol. 126, No. 12
- Detection and Identification of Toxic Microalgae by the Use of Innovative Molecular Methods
  30 January 2014
- Detection and Characterization of Leishmania (Leishmania) and Leishmania (Viannia) by SYBR Green-Based Real-Time PCR and High Resolution Melt Analysis Targeting Kinetoplast Minicircle DNA
  13 February 2014 | PLoS ONE, Vol. 9, No. 2
- p53-Dependent suppression of genome instability in germ cells
  Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis, Vol. 760
- Allele frequency of hereditary equine regional dermal asthenia in American Quarter horses in Brazil determined by quantitative real-time PCR with high resolution melting analysis
  The Veterinary Journal, Vol. 199, No. 2
- Rapid differentiation of Dirofilaria immitis and Dirofilaria repens in canine peripheral blood by real-time PCR coupled to high resolution melting analysis
  Veterinary Parasitology, Vol. 200, No. 1-2
- Genotyping of Listeria monocytogenes isolates from poultry carcasses using high resolution melting (HRM) analysis
  6 May 2014 | Biotechnology & Biotechnological Equipment, Vol. 28, No. 1
- Detection of hepatitis C virus by single-step hairpin primer RT-PCR
  Bioorganic & Medicinal Chemistry Letters, Vol. 24, No. 1
- Synthesis and evaluation of a photoresponsive quencher for fluorescent hybridization probes
  1 January 2014 | Org. Biomol. Chem., Vol. 12, No. 39
- KRAS testing in metastatic colorectal carcinoma: challenges, controversies, breakthroughs and beyond
  10 September 2013 | Journal of Clinical Pathology, Vol. 67, No. 1
- Development and assessment of multiplex high resolution melting assay as a tool for rapid single-tube identification of five Brucella species
  BMC Research Notes, Vol. 7, No. 1
- SPOP Mutations in Prostate Cancer across Demographically Diverse Patient Cohorts
  Neoplasia, Vol. 16, No. 1
- Formalin-Fixed Paraffin-Embedded Tissue (FFPET) Sections for Nucleic Acid-Based Analysis in Biomarker Discovery and Early Drug Development
  5 February 2015
- Genotyping by High-Resolution Melting Analysis
  7 April 2014
- Rapid Detection and Identification of Nontuberculous Mycobacterial Pathogens in Fish by Using High-Resolution Melting Analysis
  Applied and Environmental Microbiology, Vol. 79, No. 24
- Detection of plant oil DNA using high resolution melting (HRM) post PCR analysis: A tool for disclosure of olive oil adulteration
  Food Chemistry, Vol. 141, No. 4
- High Resolution Melting analysis as a rapid and efficient method of screening for small mutations in the STK11gene in patients with Peutz-Jeghers syndrome
  30 May 2013 | BMC Medical Genetics, Vol. 14, No. 1
- High resolution melting analysis: rapid and precise characterisation of recombinant influenza A genomes
  12 September 2013 | Virology Journal, Vol. 10, No. 1
- Detection of Hb H Disease Genotypes Common in Northern Thailand by Quantitative Real-Time Polymerase Chain Reaction and High Resolution Melting Analyses
  20 August 2013 | Hemoglobin, Vol. 37, No. 6
- Identification and differentiation of Phyllosticta species causing freckle disease of banana using high resolution melting ( HRM ) analysis
  29 March 2013 | Plant Pathology, Vol. 62, No. 6
- Rapid detection and identification of Theileria equi and Babesia caballi by high-resolution melting (HRM) analysis
  29 August 2013 | Parasitology Research, Vol. 112, No. 11
- A novel closed-tube method based on high resolution melting (HRM) analysis for authenticity testing and quantitative detection in Greek PDO Feta cheese
  Food Chemistry, Vol. 141, No. 2
- The post illumination pupil response is reduced in seasonal affective disorder
  Psychiatry Research, Vol. 210, No. 1
- Major Emerging Problems with Minor Meloidogyne Species
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- Quantum Method for Fluorescence Background Removal in DNA Melting Analysis
  26 September 2013 | Analytical Chemistry, Vol. 85, No. 20
- The quantitative real-time PCR applications in the monitoring of marine harmful algal bloom (HAB) species
  18 December 2012 | Environmental Science and Pollution Research, Vol. 20, No. 10
- Universal digital high-resolution melt: a novel approach to broad-based profiling of heterogeneous biological samples
  9 August 2013 | Nucleic Acids Research, Vol. 41, No. 18
- High‐resolution DNA melt‐curve analysis for cost‐effective mass screening of pairwise species interactions
  23 July 2013 | Molecular Ecology Resources, Vol. 13, No. 5
- Dysembryoplastic Neuroepithelial Tumors Share with Pleomorphic Xanthoastrocytomas and Gangliogliomas BRAF V600E Mutation and Expression
  20 March 2013 | Brain Pathology, Vol. 23, No. 5
- Single-step intercalating dye strategies for DNA damage studies
  Journal of Microbiological Methods, Vol. 94, No. 2
- RNAtips: analysis of temperature-induced changes of RNA secondary structure
  12 June 2013 | Nucleic Acids Research, Vol. 41, No. W1
- Strategies for Mutation Discovery in Retinitis Pigmentosa: Transition to the Next Generation
  Journal of Genetic Medicine, Vol. 10, No. 1
- High-Resolution Melt Analysis
  13 May 2013
- Fluorescent p-substituted-phenyl-imidazolo-cytidine analogues
  Tetrahedron, Vol. 69, No. 18
- High‐resolution melting analysis for bird sexing: a successful approach to molecular sex identification using different biological samples
  25 February 2013 | Molecular Ecology Resources, Vol. 13, No. 3
- Identification of C ulex complex species using SNP markers based on high‐resolution melting analysis
  26 February 2013 | Molecular Ecology Resources, Vol. 13, No. 3
- Taxonomic Identification of Mediterranean Pines and Their Hybrids Based on the High Resolution Melting (HRM) and trnL Approaches: From Cytoplasmic Inheritance to Timber Tracing
  5 April 2013 | PLoS ONE, Vol. 8, No. 4
- Analysis of partial AZFc deletions in Malaysian infertile male subjects
  12 December 2012 | Systems Biology in Reproductive Medicine, Vol. 59, No. 2
- Functional molecular markers and high-resolution melting curve analysis of low phytic acid mutations for marker-assisted selection in rice
  15 November 2012 | Molecular Breeding, Vol. 31, No. 3
- Methodological streamlining of SNP discovery and genotyping via high-resolution melting analysis (HRMA) in non-model species
  Marine Genomics, Vol. 9
- Simple and rapid discrimination of embB codon 306 mutations in Mycobacterium tuberculosis clinical isolates by a real-time PCR assay using an LNA-TaqMan probe
  Journal of Microbiological Methods, Vol. 92, No. 3
- Screening and surveillance for colorectal cancer: state of the art
  Gastrointestinal Endoscopy, Vol. 77, No. 3
- Ultralocalized thermal reactions in subnanoliter droplets-in-air
  11 February 2013 | Proceedings of the National Academy of Sciences, Vol. 110, No. 9
- Alternative Reliable Method for Cytochrome P450 2D6 Poor Metabolizers Genotyping
  26 February 2012 | Molecular Biotechnology, Vol. 53, No. 1
- High resolution melting analysis for quantitative detection of bovine milk in pure water buffalo mozzarella and other buffalo dairy products
  International Dairy Journal, Vol. 28, No. 1
- Impact of Mutation Type and Amplicon Characteristics on Genetic Diversity Measures Generated Using a High-Resolution Melting Diversity Assay
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- Ultrasensitive single-nucleotide polymorphism detection using target-recycled ligation, strand displacement and enzymatic amplification
  Nanoscale, Vol. 5, No. 11
- Rapid Molecular Typing of Prototheca zopfii by High Resolution Melting Real-Time PCR (PCR-HRM)
  Medical Mycology Journal, Vol. 54, No. 4
- Molecular Differentiation of Opisthorchis viverrini and Clonorchis sinensis Eggs by Multiplex Real-Time PCR with High Resolution Melting Analysis
  31 December 2013 | The Korean Journal of Parasitology, Vol. 51, No. 6
- Identification of Traits, Genes, and Crops of the Future
  3 May 2013
- SNP Analysis Using a Molecular Beacon-Based Operating Cooperatively (OC) Sensor
  2 July 2013
- Microsatellites: Evolution and Contribution
  15 March 2013
- Validation of a quantitative PCR-high-resolution melting protocol for simultaneous screening of COL1A1 and COL1A2 point mutations and large rearrangements: Application for diagnosis of osteogenesis imperfecta
  18 July 2012 | Human Mutation, Vol. 33, No. 12
- A novel high-resolution melting analysis-based method for Yersinia pseudotuberculosis genotyping
  Journal of Microbiological Methods, Vol. 91, No. 3
- Real-time RT-PCR high-resolution melting curve analysis and multiplex RT-PCR to detect and differentiate grapevine leafroll-associated virus 3 variant groups I, II, III and VI
  27 September 2012 | Virology Journal, Vol. 9, No. 1
- Rapid multiplex high resolution melting method to analyze inflammatory related SNPs in preterm birth
  26 January 2012 | BMC Research Notes, Vol. 5, No. 1
- Current mutation discovery approaches in Retinitis Pigmentosa
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- Application of Tm-shift genotyping method in genetic studies
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- Application of HRM assays with EvaGreen dye for genotyping Giardia duodenalis zoonotic assemblages
  11 August 2012 | Parasitology Research, Vol. 111, No. 5
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  23 October 2012 | British Journal of Cancer, Vol. 107, No. 11
- High‐resolution melting analysis: a genotyping tool for population studies on Daphnia
  28 August 2012 | Molecular Ecology Resources, Vol. 12, No. 6
- Rapid detection and simultaneous molecular profile characterization of Acanthamoeba infections
  Diagnostic Microbiology and Infectious Disease, Vol. 74, No. 2
- Identification of biomarkers to distinguish clear cell sarcoma from malignant melanoma
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- A High-Resolution Melting Protocol for Rapid and Accurate Differential Diagnosis of Thyroid Nodules
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- High-resolution melt PCR analysis for rapid identification of Chlamydia abortus live vaccine strain 1B among C. abortus strains and field isolates
  Journal of Microbiological Methods, Vol. 90, No. 3
- Molecular sexing of birds: A comparative review of polymerase chain reaction (PCR)-based methods
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- Development of a High-Resolution Melting–Based Approach for Efficient Differentiation Among Bacillus cereus Group Isolates
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- Strain-level characterization of nonstarter lactic acid bacteria in Norvegia cheese by high-resolution melt analysis
  Journal of Dairy Science, Vol. 95, No. 9
- High-Resolution Melting Analysis as a Sensitive Prescreening Diagnostic Tool to Detect KRAS, BRAF, PIK3CA, and AKT1 Mutations in Formalin-Fixed, Paraffin-Embedded Tissues
  Archives of Pathology & Laboratory Medicine, Vol. 136, No. 9
- High resolution melting analysis for detection of variable number of tandem repeats polymorphism of XRCC5
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- High-resolution melting analysis for rapid detection of linezolid resistance (mediated by G2576T mutation) in Staphylococcus epidermidis
  Journal of Microbiological Methods, Vol. 90, No. 2
- KRAS mutation detection by high-resolution melting analysis significantly predicts clinical benefit of cetuximab in metastatic colorectal cancer
  17 July 2012 | British Journal of Cancer, Vol. 107, No. 4
- Closed tube method for rapid screening of IL28B polymorphisms involved in response to hepatitis C treatment
  Molecular and Cellular Probes, Vol. 26, No. 4
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- Detection of genetic variation in KCNQ1 gene by high-resolution melting analysis in a prospective-based series of postmortem negative sudden death: comparison of results obtained in fresh frozen and formalin-fixed paraffin-embedded tissues
  9 March 2012 | International Journal of Legal Medicine, Vol. 126, No. 4
- High-resolution melting analysis of 15 genes in 60 patients with cytochrome-c oxidase deficiency
  17 May 2012 | Journal of Human Genetics, Vol. 57, No. 7
- Insertion/deletion polymorphisms are convenient and reliable markers to assess chromosomal instability in human tumors
  15 January 2018 | The International Journal of Biological Markers, Vol. 27, No. 3
- Lateral flow dipstick test for genotyping of 15 beta-globin gene (HBB) mutations with naked-eye detection
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- New Strategy for Rapid Diagnosis and Characterization of Keratomycosis
  Ophthalmology, Vol. 119, No. 5
- Scanning of mutations in short amplicons: Optimization of DNA melting method
  14 June 2012 | Molecular Biology, Vol. 46, No. 3
- Rapid Detection of G1138A and G1138C Mutations of the FGFR3 Gene in Patients with Achondroplasia Using High-Resolution Melting Analysis
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- Rapid lactic acid bacteria identification in dairy products by high-resolution melt analysis of DGGE bands
  15 February 2012 | Letters in Applied Microbiology, Vol. 54, No. 4
- A review of the application of molecular genetics for fisheries management and conservation of sharks and rays
  12 April 2012 | Journal of Fish Biology, Vol. 80, No. 5
- Fiber‐Optic High‐Resolution Genetic Screening Using Gold‐Labeled Gene Probes
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- Minim Typing – A Rapid and Low Cost MLST Based Typing Tool for Klebsiella pneumoniae
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- Conclusion and Prospects in Musa Research
  9 March 2012
- Molecular Marker Techniques in Musa Genomic Research
  9 March 2012
- Identification of rare and frequent variants of the CASR gene by high-resolution melting
  Clinica Chimica Acta, Vol. 413, No. 5-6
- Targeting DNA damage and repair: Embracing the pharmacological era for successful cancer therapy
  Pharmacology & Therapeutics, Vol. 133, No. 3
- The use of high resolution melting analysis to detect Fabry mutations in heterozygous females via dry bloodspots
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- High-Resolution Melting and Real-Time Pcr for Quantification and Detection of Drug-Resistant HBV Mutants in a Single Amplicon
  1 February 2012 | Antiviral Therapy, Vol. 17, No. 2
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  28 November 2011 | Cancer Science, Vol. 103, No. 2
- Evaluation of High‐resolution Melting Curve Analysis as a New Tool for Root‐knot Nematode Diagnostics
  16 November 2011 | Journal of Phytopathology, Vol. 160, No. 2
- Identification of the GST-T1 and GST-M1 Null Genotypes Using High Resolution Melting Analysis
  21 December 2011 | Chemical Research in Toxicology, Vol. 25, No. 1
- Application of High-Resolution DNA Melting for Genotyping in Lepidopteran Non-Model Species: Ostrinia furnacalis (Crambidae)
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- Rapid identification of CYP2C8 polymorphisms by high resolution melting analysis
  Clinica Chimica Acta, Vol. 413, No. 1-2
- High resolution melting PCR to differentiate Mycobacterium avium subsp. paratuberculosis“cattle type” and “sheep type”
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- Establishing high resolution melting analysis: method validation and evaluation for c-RET proto-oncogene mutation screening
  Clinical Chemistry and Laboratory Medicine, Vol. 50, No. 1
- Screening for mutations in the α-globin genes leading to abnormal hemoglobin variants with high resolution melting analysis
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- Molecular Genetic Testing in the Genomic Era
  2 December 2011
- High‐Resolution Melting technology: a new tool for studying the Wolbachia endosymbiont diversity in the field
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- PROSPECTS OF THE APPLICATION OF GENE DIAGNOSTICS IN MEDICINE
  28 December 2011 | Health and Ecology Issues, No. 4
- Is High Resolution Melting Analysis (HRMA) Accurate for Detection of Human Disease-Associated Mutations? A Meta Analysis
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- DNA melting analysis: Application of the “open tube” format for detection of mutant KRAS
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- A high-throughput protocol for mutation scanning of the BRCA1 and BRCA2genes
  24 June 2011 | BMC Cancer, Vol. 11, No. 1
- High resolution melting analysis for a rapid identification of heterozygous and homozygous sequence changes in the MUTYH gene
  21 July 2011 | BMC Cancer, Vol. 11, No. 1
- SYTO dyes and EvaGreen outperform SYBR Green in real-time PCR
  28 July 2011 | BMC Research Notes, Vol. 4, No. 1
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- Use of High-Resolution Melting and Melting Temperature-shift assays for specific detection and identification of Bacillus anthracis based on single nucleotide discrimination
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  15 August 2011 | Integrative Biology, Vol. 3, No. 9
- A mitochondrial species identification assay for Australian blacktip sharks ( Carcharhinus tilstoni , C. limbatus and C. amblyrhynchoides ) using real‐time PCR and high‐resolution melt analysis
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  29 June 2011 | The Journal of Headache and Pain, Vol. 12, No. 4
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  10 June 2011
- DNA amplification techniques in pharmacogenomics
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- Evaluation of DNA melting analysis as a tool for species identification
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  4 February 2011 | Nucleic Acids Research, Vol. 39, No. 8
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  9 January 2014 | Expert Review of Molecular Diagnostics, Vol. 11, No. 3
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  29 March 2011 | Biotechnology Progress, Vol. 27, No. 2
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- A comparison of restriction fragment length polymorphism, tetra primer amplification refractory mutation system PCR and unlabeled probe melting analysis for LTA+252 C>T SNP genotyping
  Clinica Chimica Acta, Vol. 412, No. 5-6
- Genotyping UGT1A1(TA) polymorphism rare variants by high resolution melting curve analysis
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  6 February 2011 | Cancer Epidemiology, Biomarkers & Prevention, Vol. 20, No. 2
- Association of an intronic SNP of SLC2A9 gene with serum uric acid levels in the Chinese male Han population by high-resolution melting method
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  The Journal of Molecular Diagnostics, Vol. 13, No. 1
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- A rapid real-time PCR/DNA resolution melting method to identify Prototheca species
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- Association of Common Polymorphisms in the Fractalkine Receptor ( CX3CR1 ) With Obesity
  6 September 2012 | Obesity, Vol. 19, No. 1
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  16 March 2011
- Rapid Identification of Common β-Thalassemia Mutations in the Chinese Population Using Duplex or Triplex Amplicon Genotyping by High-Resolution Melting Analysis
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- A One-Step Prescreening for Point Mutations and Large Rearrangement in BRCA1 and BRCA2 Genes Using Quantitative Polymerase Chain Reaction and High-Resolution Melting Curve Analysis
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  Journal of Clinical Microbiology, Vol. 48, No. 9
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  Fertility and Sterility, Vol. 94, No. 4
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  Laboratory Investigation, Vol. 90, No. 9
- Gene Network Disruptions and Neurogenesis Defects in the Adult Ts1Cje Mouse Model of Down Syndrome
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- Use of genome-wide SNP homozygosity mapping in small pedigrees to identify new mutations in VSX2 causing recessive microphthalmia and a semidominant inner retinal dystrophy
  23 April 2010 | Human Genetics, Vol. 128, No. 1
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  5 November 2009 | Diagnostic Cytopathology, Vol. 38, No. 7
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  18 May 2010 | Journal of Inherited Metabolic Disease, Vol. 33, No. 3
- Accurate quantification of dystrophin mRNA and exon skipping levels in Duchenne Muscular Dystrophy
  10 May 2010 | Laboratory Investigation, Vol. 10
- Development of a high-resolution melting method for the detection of hemoglobin alpha variants
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- Rapid Genetic Analysis of X-Linked Chronic Granulomatous Disease by High-Resolution Melting
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Vol. 8, No. 6

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Published online 11 June 2007

Published in print June 2007

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Disclosure

Aspects of high-resolution melting are licensed from the University of Utah to Idaho Technology. CTW has equity interest in Idaho Technology. GHR and JOK have nothing to disclose.

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High-resolution DNA melting analysis for simple and efficient molecular diagnostics

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