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Rapid and Sensitive HPLC Method for the Simultaneous Determination of Paraquat and Diquat in Human Serum

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Abstract

A rapid and sensitive HPLC method for the simultaneous determination of paraquat and diquat in human serum has been developed. After deproteinization of the serum with 10% trichloroacetic acid, the samples were separated on a reversed- phase column, and subsequently reduced to their radicals with alkaline sodium hydrosulfite solution. These radicals were monitored with a UV detector at 391 nm. This method permitted the reliable quantification of paraquat over linear ranges of 50 ng - 10 pg/ml and 100 ng - 10 pg/ml for diquat in human serum. The within- and between-day variations are lower than 2.3 and 2.2%, respectively. This technique was also utilized to determine the paraquat and diquat serum levels in a patient who had ingested herbicide (Prigrox L®) containing paraquat and diquat.

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Authors and Affiliations

  1. Faculty of Pharmaceutical Sciences, Fukuoka University, Jonan, Fukuoka, 814-0180, Japan

    Shuuji Hara, Noriaki Sasaki, Daizi Takase & Shouichi Shiotsuka

  2. Department of Pharmacy, Fukuoka University Hospital, Jonan, Fukuoka, 814-0180, Japan

    Kentaro Ogata & Koujiro Futagami

  3. Internal Medicine, Fukuoka University Hospital, Jonan, Fukuoka 814–0180, Japan

    Kazuo Tamura

Authors
  1. Shuuji Hara
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  2. Noriaki Sasaki
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  3. Daizi Takase
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  4. Shouichi Shiotsuka
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  5. Kentaro Ogata
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  6. Koujiro Futagami
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  7. Kazuo Tamura
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Correspondence to Shuuji Hara.

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Hara, S., Sasaki, N., Takase, D. et al. Rapid and Sensitive HPLC Method for the Simultaneous Determination of Paraquat and Diquat in Human Serum. ANAL. SCI. 23, 523–526 (2007). https://doi.org/10.2116/analsci.23.523

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  • DOI: https://doi.org/10.2116/analsci.23.523

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