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Determination of Bisphenol A and 4-Nonylphenol in Human Milk Using Alkaline Digestion and Cleanup by Solid-Phase Extraction

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Abstract

A highly sensitive and selective method based on alkaline digestion for the simultaneous determination of bisphenol A (BPA) and 4-nonylphenol (NP) was developed. The method consists of digestion of the matrix with ethanolic KOH, extraction with diethyl ether under a mild alkaline condition, cleaning with successive aminopropyl (NH2) cartridges and derivatization followed by a GC-MS analysis. The assay accuracies, expressed as recoveries, were 82 - 113% for BPA and 89 - 97% for NP. The limits of detection of BPA and NP were 0.09 ng/g and 0.50 ng/g, respectively. The procedure will be reliable for the trace analysis of BPA and NP in human milk, since alkaline digestion can diminish their documented association with protein.

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Authors and Affiliations

  1. National Environmental Research and Training Institute, Ministry of the Environment, 3-3 Namiki, Tokorozawa, Saitama, 359-0042, Japan

    Hiroaki Otaka

  2. National Institute for Environmental Studies, 16-9 Onogawa, Tsukuba, Ibaraki, 305-8506, Japan

    Akio Yasuhara & Masatoshi Morita

Authors
  1. Hiroaki Otaka
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  2. Akio Yasuhara
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  3. Masatoshi Morita
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Correspondence to Hiroaki Otaka.

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Otaka, H., Yasuhara, A. & Morita, M. Determination of Bisphenol A and 4-Nonylphenol in Human Milk Using Alkaline Digestion and Cleanup by Solid-Phase Extraction. ANAL. SCI. 19, 1663–1666 (2003). https://doi.org/10.2116/analsci.19.1663

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  • DOI: https://doi.org/10.2116/analsci.19.1663

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