Czech J. Food Sci., 2007, 25(2):81-89 | DOI: 10.17221/753-CJFS

Screening of antimicrobial activity of essential oil and methanol extract of Satureja hortensis on foodborne bacteria and fungi

Ahmet Adiguzel1, Hakan Ozer2, Hamdullah Kilic3, Bulent Cetin4
1 Biotechnology Application and Research Center,
2 Department of Field Crops, Faculty of Agriculture,

The present work reports the in vitro antimicrobial activities of the essential oil and methanol extract from Satureja hortensis as well as the content of its essential oil. The chemical composition of hydrodistilled essential oil of Satureja hortensis was analysed by means of GC-MS. Thirty constituents were identified. The main constituents of the oil were thymol (40.54%), γ-terpinene (18.56%), carvacrol (13.98%), and p-cymene (8.97). The essential oil of Satureja hortensis exhibited the activity against 25 bacteria, 8 fungi, and a yeast, C. albicans; exerting the Minimum Inhibitory Concentration values (MIC) ranging from 15.62 to 250 µl/ml. Similarly, methanol extract of the plant also showed antimicrobial activity.

Keywords: summer savory; Satureja hortensis; essential oil; methanol extract; antimicrobial activity

Published: April 30, 2007  Show citation

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Adiguzel A, Ozer H, Kilic H, Cetin B. Screening of antimicrobial activity of essential oil and methanol extract of Satureja hortensis on foodborne bacteria and fungi. Czech J. Food Sci.. 2007;25(2):81-89. doi: 10.17221/753-CJFS.
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References

  1. Adam K., Sivropoulou A., Kokkini S., Lanaras T., Arsenakis M. (1998): Antifungal activities of Origanum vulgare subsp. hirtum, Mentha spicata, Lavandula angustifolia, and Salvia fruticosa essential oils against human pathogenic fungi. Journal of Agricultural and Food Chemistry, 46: 1739-1745. Go to original source...
  2. Adams R.P. (2001): Identification of Essential Oil Components by Gas Chromatography/Quadrupole Mass Spectroscopy. Allured Publishing Corporation, Illinois.
  3. Ahmad I., Mehmood Z., Mohammad F. (1998): Screening of some Indian medicinal plants for their antimicrobial properties. Journal of Ethnopharmacology, 62: 183-193. Go to original source... Go to PubMed...
  4. Akgul A., Ozcan M., Chialva F., Monguzzi F. (1999): Essential oils of four Turkish wild-growing Labiatae herbs: Salvia cryptantha Montbr. et Auch., Satureja cuneifolia Ten., Thymbra spicata L. and Thymus cilicicus Boiss. et Bal. Journal of Essential Oil Research, 11: 209-214. Go to original source...
  5. Azaz D., Demirci F., Satil F., Kurkcuoglu M., Baser K.H.C. (2002): Antimicrobial activity of some Satureja essential oils. Zeitschrift für Naturforschung, Section C - Biosciences, 57: 817-821. Go to original source... Go to PubMed...
  6. Baher Z.F., Mirza M., Ghorbanli M., Rezaii M.B. (2002): The influence of water stress on plant height, herbal and essential oil yield and composition in Satureja hortensis L. Flavour and Fragrance Journal, 17: 275-277. Go to original source...
  7. Bandoniene D., Venskutonis P.R., Gruzdiene D., Murkovic M. (2002): Antioxidative activity of sage (Salvia officinalis L.), savory (Satureja hortensis L.) and borage (Borago officinalis L.) extracts in rapeseed oil. European Journal of Lipid Science and Technology, 104: 286-292. Go to original source...
  8. Baser K.H.C., Tumen G., Tabanca N., Demirci F. (2001): Composition and antibacterial activity of the essential oils from Satureja wiedemanniana (Lallem.) Velen. Zeitschrift für Naturforschung Section C - Biosciences, 56: 731-38. Go to original source... Go to PubMed...
  9. Baser K.H.C., Ozek T., Kirimer N., Tumen G. (2004): Comparative study of the essential oils of wild and cultivated Satureja hortensis L. Journal of Essential Oil Research, 16: 422-424. Go to original source...
  10. Baytop T. (1997): Türkçe Bitki Adlari Sözlüğü (A Dictionary of Vernacular Names of Wild Plants of Turkey). Publication of Turkish Language Society, Ankara.
  11. Chalchat J.C., Gorunovic M.S., Maksimovic Z.A. (1999): Essential oil of Satureja kitaibelii Wierzb. f. aristata (Vand.) Hayek, Lamiaceae from eastern Serbia. Journal of Essential Oil Research, 11: 691-692. Go to original source...
  12. Ciani M., Menghini L., Mariani F., Pagiotti R., Menghini A., Fatichenti F. (2000): Antimicrobial properties of essential oil of Satureja montana L. on pathogenic and spoilage yeasts. Biotechnology Letters, 22: 1007-1010. Go to original source...
  13. Cosentino S., Tuberoso C.I.G., Pisano B., Satta M., Mascia V., Arzedi E., Palmas F. (1999): In-vitro antimicrobial activity and chemical composition of Sardinian Thymus essential oils. Letters in Applied Microbiology, 29: 130-135. Go to original source... Go to PubMed...
  14. Cox S.D., Mann C.M., Markham J.L., Bell H.C., Gustafson J.E., Warmington J.R., Wyllie S.G. (2000): The mode of antimicrobial action of the essential oil of Melaleuca alternifolia. Journal of Applied Microbiology, 88: 170-175. Go to original source... Go to PubMed...
  15. Dardioti A., Cook C.M., Kokkini S., Lanaras T. (1997): Composition of Satureja horvatii subsp. macrophylla oil isolated by hydrodistillation and micro-simultaneous distillation/extraction. Journal of Essential Oil Research, 9: 663-666. Go to original source...
  16. Davis P.H. (1982): Flora of Turkey and the East Aegean Islands. Vol. 7. University Press, Edinburgh: 349-382.
  17. Deans S.G., Svoboda K.P. (1990): The antimicrobial properties of marjoram (Origanim majorana L.) volatile oil. Flavour and Fragrance Journal, 5: 187-190. Go to original source...
  18. Didry N., Dubreuil L., Pinkas M. (1993): Antimicrobial activity of thymol, carvacrol and cinnamaldehyde alone or in combination. Pharmazie, 48: 301-304. Go to PubMed...
  19. Dorman H.J.D., Deans S.G. (2000): Antimicrobial agents from plants: antibacterial activity of plant volatile oils. Journal of Applied Microbiology, 88: 308-316. Go to original source... Go to PubMed...
  20. Dorman H.J.D., Hiltunen R. (2004): Fe(III) reductive and free radical-scavenging properties of summer savory (Satureja hortensis L.) extract and subfractions. Food Chemistry, 88: 193-199. Go to original source...
  21. Eloff J.N. (1998): Which extractant should be used for the screening and isolation of antimicrobial components from plants? Journal of Ethnopharmacology, 60: 1-8. Go to original source... Go to PubMed...
  22. Exarchou V., Nenadis N., Tsimidou M., Gerothanassis I.P., Troganis A., Boskou D. (2002): Antioxidant activities and phenolic composition of extracts from Greek oregano, Greek sage, and summer savory. Journal of Agricultural and Food Chemistry, 50: 5294-5299. Go to original source... Go to PubMed...
  23. Farag R.S., Daw Z.Y., Abo-Raya S.H. (1989): Influence of some spice essential oils on Aspergillus parasiticus growth and production of aflatoxins in a synthetic medium. Journal of Food Science, 54: 74-76. Go to original source...
  24. Ghannadi A. (2002): Composition of the essential oil of Satureja hortensis L. seeds from Iran. Journal of Essential Oil Research, 14: 35-36. Go to original source...
  25. Gul H.I., Ojanen T., Hanninen O. (2002): Antifungal evaluation of bis Mannich bases derived from acetophenones and their corresponding piperidinols and stability studies. Biological Pharmaceutical Bulletin, 25: 1307-1310. Go to original source... Go to PubMed...
  26. Gulluce M., Sokmen M., Daferera D., Agar G., Ozkan H., Kartal N., Polissiou M., Sokmen A., Sahin F. (2003): In vitro antibacterial, antifungal, and antioxidant activities of the essential oil and methanol extracts of herbal parts and callus cultures of Satureja hortensis L. Journal of Agriculture and Food Chemistry, 51: 3958-3965. Go to original source... Go to PubMed...
  27. Gulluce M., Adiguzel A., Ogutcu H., Sengul M., Karaman I., Sahin F. (2004a): Antimicrobial effects of Quercus ilex L. extract. Phytotheraphy Research, 18: 208-211. Go to original source... Go to PubMed...
  28. Gulluce M., Sokmen M., Sahin F., Sokmen A., Adiguzel A., Ozer H. (2004b): Biological activities of the essential oil and methanolic extract of Micromeria fruticosa (L.) Druce ssp. serpyllifolia (Bieb) PH Davis plants from the eastern Anatolia region of Turkey. Journal of the Science of Food and Agriculture, 84: 735-741. Go to original source...
  29. Hajhashemi V., Sadraei H., Ghannadi A.R., Mohseni M. (2000): Antispasmodic and antidiarrheal effect of Satureja hortensis L. essential oil. Journal of Ethnopharmacology, 71: 187-192. Go to original source... Go to PubMed...
  30. Helander I.M., Alakomi H.L., Latva-Kala K., Mattila-Sandholm T., Pol I., Smid E.J., Gorris L.G.M., Von Wright A. (1998): Characterization of the action of selected essential oil components on gram-negative bacteria. Journal of Agricultural and Food Chemistry, 46: 3590-3595 Go to original source...
  31. Karaman I., Sahin F., Gulluce M., Ogutcu H., Sengul M., Adiguzel A. (2003): Antimicrobial activity of aqueous and methanol extracts of Juniperus oxycedrus L. Journal of Ethnopharmacology, 85: 231-235. Go to original source... Go to PubMed...
  32. Kim S.Y., Kim J.H., Kim S.K., Oh M.J., Jung M.Y. (1994): Antioxidant activities of selected oriental herb extracts. Journal of the American Oil Chemistry Society, 71: 633-640. Go to original source...
  33. Konakchiev A., Tsankova E. (2002): The essential oils of Satureja montana ssp. kitaibelii Wierzb. and Satureja pilosa var. pilosa Velen from Bulgaria. Journal of Essential Oil Research, 14: 120-121. Go to original source...
  34. Kurkcuoglu M., Tumen G., Baser K.H.C. (2001): Essential oil constituents of Satureja boissieri from Turkey. Chemistry of Natural Compounds, 37: 329-331. Go to original source...
  35. Lin J., Opoku A.R., Geheeb-Keller M., Hutchings A.D., Terblanche S.E., Jager A.K., Van Staden J. (1999): Preliminary screening of some traditional Zulu medicinal plants for anti-inflammatory and antimicrobial activities. Journal of Ethnopharmacology, 68: 267-274. Go to original source... Go to PubMed...
  36. Madsen H.L., Sorensen B., Skibsted L.H., Bertelsen G. (1998): The antioxidative activity of summer savory (Satureja hortensis L.) and rosemary (Rosmarinus officinalis L.) in dressing stored exposed to light or in darkness. Food Chemistry, 63: 173-180. Go to original source...
  37. Marino M., Bersani C., Comi G. (2001): Impedance measurements to study the antimicrobial activity of essential oils from Lamiaceae and Compositae. International Journal of Food Microbiology, 67: 187. Go to original source... Go to PubMed...
  38. Muller R.F., Berger B., Yegen O. (1995): Chemical composition and fungitoxic properties to phytopathogenic fungi of essential oils of selected aromatic plants growing wild in Turkey. Journal of Agriculture and Food Chemistry, 43: 2262-2266. Go to original source...
  39. Murray P.R., Baron E.J., Pfaller M.A., Tenover F.C., Yolke R.H. (1995): Manual of Clinical Microbiology. 6th Ed. ASM, Washington.
  40. Ozcan M., Erkmen O. (2001): Antimicrobial activity of the essential oils of Turkish plant spices. European Food Research Technology, 212: 658-660. Go to original source...
  41. Ozer H., Sokmen M., Gulluce M., Adiguzel A., Kilic H., Sahin F. Sokmen A., Baris O. (2006): In vitro antimicrobial and antioxidant activities of the essential oils and methanol extracts of Hyssopus officinalis L. ssp. angustifolius. Italian Journal of Food Science, 18: 73-83.
  42. Sahin F., Karaman I., Gulluce M., Ogutcu H., Sengul M., Adiguzel A., Ozturk S., Kotan R. (2003): Evaluation of antimicrobial activities of Satureja hortensis L. Journal of Ethnopharmacology, 87: 61-65. Go to original source... Go to PubMed...
  43. Sajjadi S.E., Baluchi M. (2002): Chemical composition of the essential oil of Satureja boissieri Hausskn. ex Boiss. Journal of Essential Oil Research, 14: 49-50. Go to original source...
  44. Sefidkon F., Ahmadi S. (2000): Essential oil of Satureja khuzistanica Jamzad. Journal of Essential Oil Research, 12: 427-428. Go to original source...
  45. Sefidkon F., Jamzad Z. (2000): Essential oil of Satureja bachtiarica Bunge. Journal of Essential Oil Research, 12: 545-546. Go to original source...
  46. Sivropoulou A., Papanikolaou E., Nikolaou C., Kokkini S., Lanaras T., Arsenakis M. (1996): Antimicrobial and cytotoxic activities of Origanum essential oils. Journal of Agricultural and Food Chemistry, 44: 1202-1205. Go to original source...
  47. Slavkovska V., Jancic R., Milosavljevic S., Djokovic D. (1997): Variability of the essential oil composition of the species Satureja montana L. (Lamiaceae). Journal of Essential Oil Research, 9: 629-634. Go to original source...
  48. Sokovic M., Tzakou O., Pitarokili D., Couladis M. (2002): Antifungal activities of selected aromatic plants growing wild in Greece. Nahrung, 46: 317-320. Go to original source... Go to PubMed...
  49. Tumen G., Kiiriimer N., Ermin N., Baser K.H.C. (1998): The essential oil of Satureja cuneifolia. Planta Medica, 64: 81-83. Go to original source... Go to PubMed...
  50. Ultee A., Bennik M.H.J., Moezelaar R. (2002): The phenolic hydroxyl group of carvacrol is essential for action against the foodborne pathogen Bacillus cereus. Applied and Environmental Microbiology, 68: 1561-1568. Go to original source... Go to PubMed...
  51. Zargari A. (1990): Medicinal Plants. Vol. IV. Tehran University Press, Tehran: 325-328.
  52. Zgoda J.R., Porter J.R. (2001): A convenient microdilution method for screening natural products against bacteria and fungi. Pharmaceutical Biology, 39: 221-225. Go to original source...

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