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Induced pluripotent stem cells: origins, applications, and future perspectives

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Abstract

Embryonic stem (ES) cells are widely used for different purposes, including gene targeting, cell therapy, tissue repair, organ regeneration, and so on. However, studies and applications of ES cells are hindered by ethical issues regarding cell sources. To circumvent ethical disputes, great efforts have been taken to generate ES cell-like cells, which are not derived from the inner cell mass of blastocyst-stage embryos. In 2006, Yamanaka et al. first reprogrammed mouse embryonic fibroblasts into ES cell-like cells called induced pluripotent stem (iPS) cells. About one year later, Yamanaka et al. and Thomson et al. independently reprogrammed human somatic cells into iPS cells. Since the first generation of iPS cells, they have now been derived from quite a few different kinds of cell types. In particular, the use of peripheral blood facilitates research on iPS cells because of safety, easy availability, and plenty of cell sources. Now iPS cells have been used for cell therapy, disease modeling, and drug discovery. In this review, we describe the generations, applications, potential issues, and future perspectives of iPS cells.

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Authors and Affiliations

  1. School of Life Sciences, Shandong University, Jinan, 250100, China

    Jing Zhao, Wen-jie Jiang, Chen Sun, Cong-zhe Hou, Xiao-mei Yang & Jian-gang Gao

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  1. Jing Zhao
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  2. Wen-jie Jiang
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  3. Chen Sun
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  4. Cong-zhe Hou
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  5. Xiao-mei Yang
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  6. Jian-gang Gao
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Corresponding authors

Correspondence to Xiao-mei Yang or Jian-gang Gao.

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The two authors contributed equally to this work

Project supported by the National Natural Science Foundation of China (Nos. 30871436, 30973297, 31171194, and 31271534), the National Basic Research Program (973) of China (Nos. 2010CB945002 and 2014CB541703), the Shandong Provincial Science and Technology Key Program (No. 2009GG10003039), and the Independent Development Foundation of Shandong University (Nos. 2012JC019 and 2012ZD030), China

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Zhao, J., Jiang, Wj., Sun, C. et al. Induced pluripotent stem cells: origins, applications, and future perspectives. J. Zhejiang Univ. Sci. B 14, 1059–1069 (2013). https://doi.org/10.1631/jzus.B1300215

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