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Abstract
We present results of an approach in which lowdensity labeled DNA itself provides an amplification of the crosscorrelated fluorescent signal in the twocolor crosscorrelation function. Tetramethylrhodamine-4- dUTP and Cy5-dCTP are incorporated by polymerase chain reaction at multiple positions of the same 217 bp t a rget DNA. We call this novel approach the twocolor FCS signal amplification. The signal amplification is an example for interactions of two ligands with diff e rent colors at multiple positions of the same target.
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Published Online: 2005-06-01
Published in Print: 2001-03-21
Copyright © 2001 by Walter de Gruyter GmbH & Co. KG
