Abstract
As a potential anticarcinogenic agent, polysaccharides from Cordyceps sinensis have been demonstrated to possess strong antioxidation activity. The aim of the present research was to study the optimal medium to produce polysaccharides of C. sinensis by using response surface methodology (RSM). The composition of optimized medium for polysaccharide production calculated from the regression model of RSM was 6.17% sucrose, 0.53% corn steep powder, 0.5% (NH4)2HPO4, and 0.15% KH2PO4 at pH 4.44, with a predicted maximum polysaccharide production of 3.17 g/L. When applying this optimal medium, the maximum polysaccharide production was 3.05 and 3.21 g/L in a shake flask and a 5-L jar fermentor, respectively. When the pH was controlled at a higher level such as pH 5.0, both cell growth and polysaccharide production were inhibited. A low pH of 2.85 was required for maximum production of polysaccharides.
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Li, Q. S., Zeng, W., Yi, D. H., and Huang, T. F. (1998), Chung Kuo Chung Yao Tsa Chih 23(4), 210–212.
Chou, Z. G. and Lin, X. (1994), Shanghai J. Immunol. 14, 30–34.
Xu, X. and Chen, G. Z. (1995), Hunan Med. 12, 202.
Zheng, F., Zheng, J., Yang, W., and Li, L. S. (1994), Chin. J. Pathophysiol. 10, 314.
Li, S. P., Zhao, K. J., Ji, Z. N., Song, Z. H., Dong, T. T. X., Lo, C. K., Cheung, J. K. H., Zhu, S. Q., and Tsim, K. W. K. (2003), Life Sci. 73, 2503–2513.
Zhu, J. S., Halpern, G. M., and Jones, K. (1998), J. Altern. Complement. Med. 4, 289–303.
Li, S. P., Li, P., Dong, T. T. X., and Tsim, K. W. K. (2001), Phytomedicine 8, 207–212.
Bok, J. W., Lermer, L., Chilton, J., Klingerman, H. G., and Towers, G. H. N. (1999), Phytochemistry 51, 891–898.
Zhong, J. J. and Tang, Y. J. (2004), in Advances in Biochemical Engineering/Biotechnology, vol. 87, Zhong, J. J., ed., Springer-Verlag, Heidelberg, pp. 25–59.
Chen, Q. H., He, G. Q., and Ali, M. A. M. (2002), Enzyme Microb. Technol. 30, 667–672.
Yang, F. C., Huang, H. C., and Yang, M. J. (2003), Enzyme Microb. Technol. 33, 395–402.
Hsieh, C. and Yang, F. C. (2004), Bioresour. Technol. 91, 105–109.
Dubois, M., Gilles, K. A., Hamilton, J. K., Rebers, P. A., and Smith, F. (1956), Anal. Chem. 28(3), 350–356.
Paterson-Beedle, M., Kennedy, J. F., Melo, F. A. D., Lloyd, L. L., and Medeiros, V. (2000), Carbohydr. Polymers 42, 375–383.
Fang, Q.-H. and Zhong, J.-J. (2002), Process Biochem. 37, 769–774.
Yang, F. C. and Liau, C. B. (1998), Bioprocess Eng. 19, 233–236.
Box, G. E. P. and Wilson, K. B. (1951), J. R. Stat. Soc. B13, 1–45.
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Hsieh, C., Tsai, MJ., Hsu, TH. et al. Medium optimization for polysaccharide production of Cordyceps sinensis . Appl Biochem Biotechnol 120, 145–157 (2005). https://doi.org/10.1385/ABAB:120:2:145
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DOI: https://doi.org/10.1385/ABAB:120:2:145