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Catalytic Assays for Human Cytochrome P450

An Introduction

  • Protocol
Cytochrome P450 Protocols

Part of the book series: Methods in Molecular Biology ((MIMB,volume 320))

Abstract

Cytochrome P450 (P450) is a superfamily of individual monooxygenase enzymes that metabolize structurally diverse xenochemicals, including many clinically useful drugs and foreign chemicals widespread in the environment. P450 substrates that can be used to selectively monitor individual P450 enzymes or P450 subfamilies have been identified through studies using P450 enzyme-selective inhibitory antibodies and chemical inhibitors in conjunction with experiments utilizing individual cDNA-expressed P450 enzymes. This chapter describes P450 form-selective substrates that can be used to monitor the activities of human P450 enzymes CYP1A, CYP1B1, CYP2A6, CYP2B6, CYP2C8, CYP2C9, CYP2C19, CYP2D6, CYP2E1, CYP3A, CYP4A11, and CYP7A1. Cautions that need to be exercised when using these substrates to probe for individual P450 activities in human liver and other tissues are discussed.

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Acknowledgments

This work was supported in part by the Canadian Institutes of Health Research (grant MOP-42385) and the National Institutes of Health (grant 5 P42 ES07381).

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Authors and Affiliations

  1. Faculty of Pharmaceutical Sciences, University of British Columbia, Vancouver, British Columbia, Canada

    Thomas K. H. Chang

  2. Division of Cell and Molecular Biology, Department of Biology, Boston University, Boston, MA, USA

    David J. Waxman

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  1. Thomas K. H. Chang
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Editors and Affiliations

  1. School of Biological Sciences, Queen Mary University of London, London, UK

    Ian R. Phillips

  2. Department of Biochemistry and Molecular Biology, University College London, London, UK

    Elizabeth A. Shephard

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Chang, T.K.H., Waxman, D.J. (2006). Catalytic Assays for Human Cytochrome P450. In: Phillips, I.R., Shephard, E.A. (eds) Cytochrome P450 Protocols. Methods in Molecular Biology, vol 320. Humana Press, Totowa, NJ. https://doi.org/10.1385/1-59259-998-2:73

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  • DOI: https://doi.org/10.1385/1-59259-998-2:73

  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-58829-441-8

  • Online ISBN: 978-1-59259-998-1

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