Skip to main content

Studies of Cerebral Vessels by Transmission Electron Microscopy and Morphometry

  • Protocol
The Blood-Brain Barrier

Part of the book series: Methods in Molecular Medicineā„¢ ((MIMM,volume 89))

  • 1717 Accesses

Abstract

Transmission electron microscopy (TEM), a once popular research tool, is used less frequently now. However, the isolation of novel proteins in the past decade has led to renewed use of electron microscopy for the subcellular localization of these proteins. This chapter will describe the standard method for preparation of brain tissue for TEM studies of cerebral vessels.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Protocol
USD 49.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 129.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 169.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

References

  1. Karnovsky, M. J. (1965) A formaldehyde-glutaraldehyde fixative of high osmolality for use in electron microscopy. J. Cell Biol. 27, 137A.

    Google ScholarĀ 

  2. Hayat, M. A. (2000) Principles and Techniques of Electron Microscopy. Biological Applications, 4th ed. Cambridge University Press, New York.

    Google ScholarĀ 

  3. Hunter E. (1993) Practical Electron Microscopy. A Beginnerā€™s Illustrated Guide, 2nd ed. Cambridge University Press, Cambridge, UK.

    Google ScholarĀ 

  4. Peachey, L. D. (1958) Thin sections. I. A study of section thickness and physical distortion produced during microtomy. J. Biophys. Biochem. Cytol. 4, 233ā€“242.

    ArticleĀ  PubMedĀ  CASĀ  Google ScholarĀ 

  5. Baumbach, G. L., Walmsley, J. G., and Hart, M. N. (1988) Composition and mechanics of cerebral arterioles in hypertensive rats. Am. J. Pathol. 133, 464ā€“471.

    PubMedĀ  CASĀ  Google ScholarĀ 

  6. Gross, P. M., Sposito, N. M., Pettersen, S. E., and Fenstermacher, J. D. (1986) Differences in function and structure of the capillary endothelium in gray matter, white matter and a circumventricular organ of rat brain. Blood Vessels 23, 261ā€“270.

    PubMedĀ  CASĀ  Google ScholarĀ 

  7. Nag, S., and Kilty, D. W. (1997) Cerebrovascular changes in chronic hypertension. Protective effects of enalapril in rats. Stroke 28, 1028ā€“1034.

    PubMedĀ  CASĀ  Google ScholarĀ 

  8. Nag, S. (1995) Role of endothelial cytoskeleton in blood-brain barrier permeability to protein. Acta Neuropathol. (Berl) 90, 454ā€“460.

    ArticleĀ  CASĀ  Google ScholarĀ 

  9. Nag S. (1998) Blood-brain barrier permeability measured with histochemistry. In Introduction to the Blood-Brain Barrier. Methodology, Biology and Pathology. Pardridge, W. M. ed. Cambridge University Press, Cambridge, UK, pp 113ā€“121.

    ChapterĀ  Google ScholarĀ 

  10. Stewart, P. A., Hayakawa, K., and Farrell C. (1994) Quantitation of blood-brain barrier ultra-structure. Microsc. Res. Tech. 27, 516ā€“527.

    ArticleĀ  PubMedĀ  CASĀ  Google ScholarĀ 

  11. Nagy, Z., Peters, H., and Huttner, I. (1983) Charge-related alterations of the cerebral endothelium. Lab. Invest. 49, 662ā€“671.

    PubMedĀ  CASĀ  Google ScholarĀ 

Download references

Author information

Authors and Affiliations

Authors

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

Ā© 2003 Humana Press Inc., Totowa, NJ

About this protocol

Cite this protocol

Nag, S. (2003). Studies of Cerebral Vessels by Transmission Electron Microscopy and Morphometry. In: Nag, S. (eds) The Blood-Brain Barrier. Methods in Molecular Medicineā„¢, vol 89. Humana Press. https://doi.org/10.1385/1-59259-419-0:37

Download citation

  • DOI: https://doi.org/10.1385/1-59259-419-0:37

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-073-1

  • Online ISBN: 978-1-59259-419-1

  • eBook Packages: Springer Protocols

Publish with us

Policies and ethics