Skip to main content
SpringerLink
Log in

A Simple Method to Measure Plasma Levels of Propafenone with Fluorescence Detection

  • Published:
Chromatographia Aims and scope Submit manuscript

Abstract

The aim of the present study was to develop a simple method to measure plasma levels of propafenone by liquid chromatography with a C18 reverse-phase column and fluorescence detection, without previous derivatization of the sample. Linearity was assessed in the range from 50 to 1000 ng mL−1 and had a correlation coefficient of 0.999. The inter- and intra-day coefficients of variation were below 5%. The limits of detection and quantification were 15 ng mL−1 and 50 ng mL−1, respectively. Drug levels were determined satisfactorily in two patients. A simple and reliable method was developed, especially useful in children with heart failure under treatment with propafenone.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Hii JT, Duff HJ, Burgess ED (1991) Clin Pharmacokinet 21:1–10

    PubMed  Google Scholar 

  • Carranza RR (1995) Propafenona. In: Vademécum Académico de Medicamentos, 2nd ed, Interamericana Mc Graw Hill, México D.F., pp. 701–702

  • de Gaitani CM, Lanchote VL, Bonato PSJ (1998) Chromatogr B Biomed Sci Appl 708:177–183

    Article  Google Scholar 

  • Ito S, Gow R, Verjee Z, Giesbrecht E, Dodo H, Freedom R, Tonn GR, Axelson JE, Zalzstein E, Rosenberg HC, Koren GJ (1998) Clin Pharmacol 38:496–501

    Google Scholar 

  • Boelkens B (2000) Pediatr Cardiol 21:508–515

    Article  PubMed  Google Scholar 

  • Hessling G, Brockmeier K, Ruediger HJ, Ulmer HE (2001) Am J Cardiol 87:802–804

    Article  PubMed  Google Scholar 

  • Janousek J, Paul T (1998) Am J Cardiol 81:1121–1124

    PubMed  Google Scholar 

  • Zalzstein E, Koren G, Bryson SM, Freedom RM (1990) J Pediatr 116:310–312

    PubMed  Google Scholar 

  • Tam W, Li Q, McKay G, Semple HA (1998) J Pharm Biomed Anal 16: 991–1003

    Article  PubMed  Google Scholar 

  • Harapat SR, Kates RE (1982) J Chromatogr Biomed Appl 230:448–453

    Article  Google Scholar 

  • Verbesselt R, Tjandramaga TB, de Schepper PJ (1991) Ther Drug Monitor 13:157–165

    Google Scholar 

  • Sasaki S, Kuomi S, Sato R, Murata M, Nagasawa K, Sakurai E, Hikichi N, Hayakawa H (1998) Gen Pharmac 31:589–591

    Google Scholar 

  • Haefeli EW, Vozeh S, Ha HR, Follath F (1990) Clin Pharmacol Ther 48:245–254

    PubMed  Google Scholar 

  • de Abreu LRP, Lanchote VL, Bertucci C, Cesarino EJ, Bonato PS (1999) J Pharm Biomed Anal 20:209–216

    Article  PubMed  Google Scholar 

  • Hofmann U, Pecia M, Heinkele G, Dilger K, Kroemer HK, Eichelbaum M (2000) J Chromatogr B 748:113–123

    Google Scholar 

  • Brode E, Kripp U, Hollmann M (1984) Drug Res 34:1455–1460

    Google Scholar 

  • Miller JC, Miller JN (1993) In: Statistics for Analytic Chemistry, 3rd ed., Ellis Horwood, Chichester

  • Norma Oficial Mexicana NOM-177-SSA1-1998, Validación de métodos analíticos para estudios de bioequivalencia, mayo de 1999. In: Diario Oficial de la Federación

  • Snyder LR, Kirkland JJ (1979) Introduction to modern liquid chromatography, 2nd ed., Wiley-Interscience, New York.

Download references

Author information

Authors and Affiliations

  1. Laboratorio de Farmacología, Instituto Nacional de Pediatría (INP), Avenida Imán No.1 3er piso, Colonia Cuicuilco, CP 04530, México D.F., México

    C. Flores-Pérez, H. Juárez-Olguín, J. Flores-Pérez & B. Ramírez-Mendiola

  2. Departamento de Farmacología, Facultad de Medicina, Universidad Nacional Autónoma de México, México

    H. Juárez-Olguín & J. Flores-Pérez

  3. Servicio de Cardiología, INP, México D.F., México

    J. Bobadilla Chávez

Authors
  1. C. Flores-Pérez
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. H. Juárez-Olguín
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. J. Flores-Pérez
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. B. Ramírez-Mendiola
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. J. Bobadilla Chávez
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to H. Juárez-Olguín.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Flores-Pérez, C., Juárez-Olguín, H., Flores-Pérez, J. et al. A Simple Method to Measure Plasma Levels of Propafenone with Fluorescence Detection. Chroma 62, 373–377 (2005). https://doi.org/10.1365/s10337-005-0650-4

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1365/s10337-005-0650-4

Keywords

Search

Navigation