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Analysis of Gene Expression in the Endocervical Epithelium of Wonnen With Deep Endometriosis

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Abstract

Endometriosis affects approximately 12% of reproductive-age women and is currently diagnosed using invasive laparoscopic sur-gery. Differences in gene expression in the eutopic endometrium between women with and without endometriosis have been reported, and determining the reproducibility of these genetic differences in the endocervical epithelium would represent an important step toward developing novel diagnostic strategies. In this study, we analyzed gene expression in the endocervical epithelium in women with and without moderate or severe endometriosis. Using RT2 Profiler PCR Arrays, we analyzed gene expression in endocervical epithelial cells from women with deep endometriosis (n = 4) and healthy women (n =6). Nine genes were identified as being upregulated:5 cell cycle genes (cyclin Bl [CCNBI], cyclin Gl [CCNGI], cullin I [CULI], general tran-scription factor IIH, Polypeptide I [GTF2HI ], and proliferating cell nuclear antigen [PCNA]), 3 cytokinegenes (C3, chemokine (C-C motif) ligand 21 [CCL2I], and chemokine (C-X-C motif) ligand 14 [CXCLI4]) and I gene related to dendritic cell pathways (ICAM2), showingthat differential gene expression is present in the endocervical epithelium of women with deep endometriosis.

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Authors and Affiliations

  1. Pelvic Pain and Endometriosis Unit, Gynecology Department, Paulista School of Medicine, Federal University of Sao Paulo (EPM-UNIFESP), Rua Napoleão de Barros, 608 Vila Clementino, São Paulo, SP, 04024-002, Brazil

    Alexander Kopelman MD, PhD, Manoel J. B. C. Girão MD, PhD, Tatiana C. S. Bonetti MD, PhD, Cristina V. Carvalho MD, PhD & Eduardo Schor MD, PhD

  2. Molecular and Metabolomics Laboratory, Department of Gynecology, Universidade Federal de Säo Paulo - Escola Paulista de Medicina (EPM-UNIFESP), São Paulo, Brazil

    Ismael Dale Cotrim Guerreiro da Silva MD, PhD

Authors
  1. Alexander Kopelman MD, PhD
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  2. Manoel J. B. C. Girão MD, PhD
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  3. Tatiana C. S. Bonetti MD, PhD
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  4. Cristina V. Carvalho MD, PhD
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  5. Ismael Dale Cotrim Guerreiro da Silva MD, PhD
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  6. Eduardo Schor MD, PhD
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Correspondence to Alexander Kopelman MD, PhD.

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Kopelman, A., Girão, M.J.B.C., Bonetti, T.C.S. et al. Analysis of Gene Expression in the Endocervical Epithelium of Wonnen With Deep Endometriosis. Reprod. Sci. 23, 1269–1274 (2016). https://doi.org/10.1177/1933719116638179

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  • DOI: https://doi.org/10.1177/1933719116638179

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