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Transcription of TIMP3, DAPK1, and AKR1B10 in squamous-cell lung cancer

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Abstract

Lung cancer is among the most common neoplasms in Russia, the United States, and in Western Europe and is accompanied by changes in the functional activity of many genes. The transcription levels of TIMP3, DAPK1, and AKR1B10 were compared for normal and tumor lung tissues of patients with squamous-cell cancer (SCC) by RT-PCR. A substantial increase in AKR1B10 transcription level was observed in 80% of the tumors. The transcription levels of TIMP3 and DAPK1 were significantly decreased in 76 and 72% of the tumors, respectively. The results implicated the genes in carcinogenesis in SCC, AKR1B10 acting as a potential oncogene, and TIMP3 and DAPK1 acting as potential tumor suppressor genes. It was assumed that dramatic changes in their transcription levels could be used for early diagnosis of SCC.

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Authors and Affiliations

  1. Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, 119991, Russia

    T. D. Mashkova, N. Yu. Oparina, O. L. Zinov’eva, E. S. Kropotova, V. I. Dubovaya, A. B. Poltaraus & L. L. Kisselev

  2. State Research Center GosNIIgenetika, Moscow, 113545, Russia

    M. V. Fridman

  3. Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, 117997, Russia

    E. P. Kopantsev, T. V. Vinogradova, M. V. Zinov’eva & E. D. Sverdlov

  4. Blokhin Cancer Research Center, Russian Academy of Medical Sciences, Moscow, 115478, Russia

    K. K. Laktionov, O. T. Kasymova & I. B. Zborovskaya

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  1. T. D. Mashkova
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Original Russian Text © T.D. Mashkova, N.Yu. Oparina, O.L. Zinov’eva, E.S. Kropotova, V.I. Dubovaya, A.B. Poltaraus, M.V. Fridman, E.P. Kopantsev, T.V. Vinogradova, M.V. Zinov’eva, K.K. Laktionov, O.T. Kasymova, I.B. Zborovskaya, E.D. Sverdlov, L.L. Kisselev, 2006, published in Molekulyarnaya Biologiya, 2006, Vol. 40, No. 6, pp. 1047–1054.

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Mashkova, T.D., Oparina, N.Y., Zinov’eva, O.L. et al. Transcription of TIMP3, DAPK1, and AKR1B10 in squamous-cell lung cancer. Mol Biol 40, 945–951 (2006). https://doi.org/10.1134/S0026893306060148

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