Abstract
Two monoclonal antibodies (mABs) raised against plum pox virus (PPV) were shown to recognize its D, M, and C strains. Conjugates of the antibodies with colloidal gold (CG) nanoparticles averaging 26 nm in diameter were synthesized. The binding constants of PPV with both the native and conjugated mABs were determined using a Biacore X device. The complexes between the CG-mAB conjugates and plum pox virions were examined by means of transmission electron and atomic force microscopy. Using the conjugates with optimal component ratio, an express immunochromatographic assay of PPV was developed with a detection limit of 3 ng/ml and duration of 10 min. The assay was tested for PPV detection in sam- ples of stone fruit tree leaves and demonstrated a good compatibility with the data obtained by “sandwich”-ELISA. The developed assay can be used in the field and applied for monitoring viral infection and for quarantine purposes.
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Salvador, B., Garcia, J. A., and Simon-Mateo, C. (2006) EPPO Bull., 36, 229–238.
Candresse, T., and Cambra, M. (2006) EPPO Bull., 36, 239–246.
Cambra, M., Capote, N., Myrta, A., and Llacer, G. (2006) EPPO Bull., 36, 202–204.
Glasa, M., and Candresse, T. (2005) Plum Pox Virus. AAB Descriptions of Plant Viruses, 410, 1–12.
Cambra, M., Boscia, D., Myrta, A., Palkovics, L., Navratil, M., Barba, M., Gorris, M. T., and Capote, N. (2006) EPPO Bull., 36, 254–261.
Olmos, A., Capote, N., and Candresse, T. (2006) EPPO Bull., 36, 262–266.
Diagnostic Protocols for Regulated Pests. Plum Pox Potyvirus (2004) EPPO Bull., 34, 247–256.
Danks, C., and Barker, I. (2000) EPPO Bull., 30, 421–426.
Salomone, A., and Roggero, P. (2002) J. Plant Pathol., 84, 65–68.
Salomone, A., Bruzzone, C., Minuto, G., Minuto, A., and Roggero, P. (2002) J. Plant Pathol., 84, 193.
Salomone, A., Mongelli, M., Roggero, P., and Boscia, D. (2004) J. Plant Pathol., 86, 43–48.
Kusano, N., Hirashima, K., Kuwahara, M., Narahara, K., Imamura, T., Mimori, T., Nakahira, K., and Torii, K. (2007) J. Gen. Plant Pathol., 73, 66–71.
Diagnostic Protocols for Regulated Pests. Tomato Spotted Wilt Tospovirus, Impatiens Necrotic Spot Tospovirus and Watermelon Silver Mottle Tospovirus (2004) EPPO Bull., 34, 271–279.
Diagnostic Protocols for Regulated Pests. Beet Necrotic Yellow Vein Virus (Benyvirus) (2006) EPPO Bull., 36, 429–440.
Safenkova, I. V., Byzova, N. A., Chirkov, S. N., Blintsov, A. N., Zherdev, A. V., Dzantiev, B. B., and Atabekov, I. G. (2008) Proc. All-Russ. Conf. “Chemical Analysis”, Moscow, pp. 77–79.
Byzova, N. A., Safenkova, I. V., Chirkov, S. N., Zherdev, A. V., Blintsov, A. N., Dzantiev, B. B., and Atabekov, I. G. (2009) Appl. Biochem. Microbiol., 45, 204–209.
Maiss, E., Timpe, U., Brisske, A., Jelkmann, W., Casper, R., Himmler, G., Mattanovich, D., and Katinger, H. W. (1989) J. Gen. Virol., 70, 513–524.
Lain, S., Riechmann, J. L., Mendez, E., and Garcia, J. A. (1988) Virus Res., 10, 325–342.
Shulman, M., Wilde, C. D., and Kohler, G. (1978) Nature, 276, 269–270.
Moser, H., and Vecchio, G. (1967) Experientia, 23, 1–10.
Lindmark, R., Thoren-Tolling, K., and Sjoquist, J. (1983) J. Immunol. Meth., 62, 1–13.
Wurzner, R., and Baumgarten, H. (1992) in Monoclonal Antibodies (Peters, J. H., and Baumgarten, H., eds.) Springer, Berlin, pp. 139–148.
Frens, G. (1973) Nature Phys. Sci., 241, 20–22.
Panerai, R. B., Chacon, M., Pereira, R., and Evans, D. H. (2004) Med. Eng. Phys., 26, 43–52.
Dzantiev, B. B., Zherdev, A. V., Popov, V. O., Vengerov, Yu. Yu., Starovoitova, T. A., and Toguzov, R. T. (2002) Klin. Lab. Diagn., 8, 25–31 [in Russ.].
Harris, L. J., Skaletsky, E., and McPherson, A. (1998) J. Mol. Biol., 275, 861–872.
Safenkova, I. V., Zherdev, A. V., and Dzantiev, B. B. (2010) J. Immunol. Meth., 357, 17–25.
Egorov, A. M., Osipov, A. P., Dzantiev, B. B., and Gavrilova, E. M. (1991) Theory and Practice of Enzyme-Linked Immunoassay [in Russian], Vysshaya Shkola, Moscow.
Glasa, M., Marie-Jeanne, V., Labonne, G., Subr, Z., Kudela, O., and Quiot, J. B. (2002) Eur. J. Plant Pathol., 108, 843–853.
James, D., Vagra, A., Thompson, D., and Hayes, S. (2003) Plant Dis., 87, 1119–1124.
Mitrofanova, O. V., Mitrofanova, I. V., Ezhov, V. N., Lesnikova-Sedoshenko, N. P., Lukicheva, L. A., Smykov, A. V., Senin, V. V., and Litvinova, T. V. (2005) Bull. Nikitsky Bot. Garden, 91, 111–120 [in Russ.].
Mitrofanova, O. V., Mitrofanova, I. V., Chirkov, S. N., Ezhov, V. N., and Lesnikova-Sedoshenko, N. P. (2009) Trudy Nikitsky Bot. Garden, 131, 94–103 [in Russ.].
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Original Russian Text © N. A. Byzova, I. V. Safenkova, S. N. Chirkov, V. G. Avdienko, A. N. Guseva, I. V. Mitrofanova, A. V. Zherdev, B. B. Dzantiev, J. G. Atabekov, 2010, published in Biokhimiya, 2010, Vol. 75, No. 11, pp. 1583–1595.
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Byzova, N.A., Safenkova, I.V., Chirkov, S.N. et al. Interaction of Plum Pox Virus with Specific Colloidal Gold-Labeled Antibodies and Development of Immunochromatographic Assay of the Virus. Biochemistry Moscow 75, 1393–1403 (2010). https://doi.org/10.1134/S000629791011012X
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DOI: https://doi.org/10.1134/S000629791011012X