Abstract
More than 20 years have passed since the Ishikawa cell line, a well-differentiated human endornetrial adenocarcinoma cell line, was established. Because this cell line bears estrogen and progesterone receptors, the cells have been used in numerous basic research areas such as reproductive biology and molecular science, and has been distributed to more than a hundred institutes. However, even the Ishikawa cells, after long-term culture, tend to transform into undifferentiated cells. In addition, it has been reported that estrogen and progesterone receptors disappeared from the cells that I distributed. I therefore attempted to establish well-differentiated cells from the parent Ishikawa cells and to produce a new and good quality supply of this cell line. I believe that it is very important for the investigator who established a cell line to be responsible for maintaining the quality of the cells. That is why I have not deposited this cell line in any cell bank.
I would like to take this opportunity to report the history of Ishikawa cells from establishment to the present.
Similar content being viewed by others
References
Nishida, M., Kasahara, K., Kaneko, M., Iwasaki, H., and Hayashi, K. Establishment of a new human endometrial adenocarcinoma cell line, Ishikawa cells, containing estrogen and progesterone receptors. Acta Obst. Gynuec. Jpn. 1985; 37: 1103–1111.
Kuramoto, H., Tamura, S., Notake, Y.: Establishment of a cell line of human endometrial adenecarcinoma in vitro. Am J Obstet Gynecol 1972; 114: 1012–1019.
McGuire, W. L., DeLaGarza, M.: Improved sensitivity in the measurement of estrogen receptor in human breast cancer. J Clin Endocrinol Metab 1973; 37: 986–989.
Nishida, M., Kasahara, K., Oki, A., Satoh, T., Arai, Y., Kubo, T. Establishment of eighteen clones of Ishikawa cells. Human Cell 1996; 9: 109–116.
McCarty, K.S. Jr., Barton, T.K., Fetter, B.F., Creasman, W.T., and McCarty, K.S. Sr. Correlation of estrogen and progesterone receptors with histologic differentiation in endometrial adenocarcinoma. Am. J Puthol. 1979; 96: 171–182.
Creasman, W.T., McCarty, K.S. Sr., Barton, T.K., and McCarty, K.S. Jr. Clinical correlates of estrogen and progesterone-binding in human endometrial adenocarcinoma. Obstet. Gynecol. 1980; 55: 363–370.
Ehrlich, C.E., Young, P.C.M., and Cleary, R.E. Cytoplasmic progesterone and estradiol receptors in normal, hyperplastic, and carcinomatous endometria: Therapeutic implications. Am. J. Obstet. Gynecol. 1981; 141: 539–546.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Nishida, M. The Ishikawa cells from birth to the present. Hum Cell 15, 104–117 (2002). https://doi.org/10.1111/j.1749-0774.2002.tb00105.x
Issue Date:
DOI: https://doi.org/10.1111/j.1749-0774.2002.tb00105.x