Abstract
Aim:
To investigate the underlying mechanism of acid-sensing ion channel (ASIC) 1a involved in the acidosis-induced cytotoxicity of rat C6 glioma cells.
Methods:
The stable ASIC1a-silenced C6 cells built with the RNA interference technology were confirmed by RT-PCR and Western blot analysis. Intracellular calcium ([Ca2+]i) in both the wild-type rat C6 glioma cells and the ASIC1a-silenced C6 cells were analyzed before and after acid application/exposure with the calcium imaging experiment.
Results:
The rapid extracellular pH drop induced the increase of [Ca2+]i in the wild-type C6 cells, but not in the ASIC1a-silenced C6 cells. During the prolonged acid exposure, [Ca2+]i was lower in the ASIC1a-silenced C6 cells than that in the control cells.
Conclusion:
The resultant toxicity of [Ca2+]i might contribute to the acidosis-induced cytotoxicity.
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Project supported by a grant from the National Natural Science Foundation of China (No 30472019 and 30500620).
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Weng, Xc., Zheng, Jq., Li, J. et al. Underlying mechanism of ASIC1a involved in acidosis-induced cytotoxicity in rat C6 glioma cells. Acta Pharmacol Sin 28, 1731–1736 (2007). https://doi.org/10.1111/j.1745-7254.2007.00708.x
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DOI: https://doi.org/10.1111/j.1745-7254.2007.00708.x
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