DNA Ligation and the Coupling of T4 Late Transcription to Replication
This extract was created in the absence of an abstract.
Excerpt
The “late” genes of several large bacterial and animal viruses cannot be fully expressed unless viral replication takes place. It has long been realized that the coupling between replication and transcription could be an important regulatory mechanism of cell cycles, of cell separation and of cell proliferation. For that reason, it is a welcome opportunity to study replication-dependent gene expression under the relatively well-explored conditions of bacteriophage development, even if it seems probable that this rather widespread phenomenon does not have a unitary mechanistic basis.
The late genes of phage T4D are known to depend on concurrent replication for their expression (Lembach et al., 1969; Riva and Geiduschek, 1969; Riva, Cascino, and Geiduschek, 1970a and b). Such a continuous coupling could reflect the properties of a DNA template that must be in a particular chemical state for the expression of certain genes (Luria, 1962). Our studies of the conditions for...
