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Laser for treatment of aphthous ulcers on bacteria cultures and DNA

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Abstract

Low-intensity red lasers are proposed for treatment of oral aphthous ulcers based on biostimulative effects. However, effects of low-intensity lasers at fluences used in clinical protocols on DNA are controversial. The aim of this work was to evaluate the effects of low-intensity red laser on survival and induction of filamentation of Escherichia coli cells, and induction of DNA lesions in bacterial plasmids. Escherichia coli cultures were exposed to laser (660 nm, 100 mW, 25 and 45 J cm−2) to study bacterial survival and filamentation. Also, bacterial plasmids were exposed to laser to study DNA lesions by electrophoretic profile and action of DNA repair enzymes. Data indicate that low-intensity red laser: (i) had no effect on survival of E. coli wild type, exonuclease III and formamidopyrimidine DNA glycosylase/MutM protein but decreased the survival of endonuclease III deficient cultures; (ii) induced bacterial filamentation, (iii) there was no alteration in the electrophoretic profile of plasmids in agarose gels, (iv) there was no alteration in the electrophoretic profile of plasmids incubated with formamidopyrimidine DNA glycosylase/MutM protein and endonuclease III enzymes, but it altered the electrophoretic profile of plasmids incubated with exonuclease III. Low-intensity red laser at therapeutic fluences has an effect on the survival of E. coli endonuclease III deficient cells, induces bacterial filamentation in E. coli cultures and DNA lesions targeted by exonuclease III.

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Authors and Affiliations

  1. Centro de Ciências da Saúde, Centro Universitário Serra dos Órgãos, Avenida Alberto Torres 111 Alto Teresópolis, Rio de Janeiro, 25964004, Brazil

    Roberta da Silva Marciano, Luiz Philippe da Silva Sergio, Giovanni Augusto Castanheira Polignano, Oscar Roberto Guimarães, Mauro Geller, Severo de Paoli, Flavia de Paoli & Adenilson de Souza da Fonseca

  2. Departamento de Ciências Fisiológicas, Instituto Biomédico, Universidade Federal do Estado do Rio de Janeiro, Rua Frei Caneca 94, Rio de Janeiro, 20211040, Brazil

    Giuseppe Antonio Presta & Adenilson de Souza da Fonseca

  3. Setor de Facomatoses do Serviço de Genética Clínica do IPPMG, Universidade Federal do Rio de Janeiro, Avenida Brigadeiro Trompowsky, Rio de Janeiro, 21949590, Brazil

    Mauro Geller

  4. Departamento de Morfologia, Instituto de Ciências Biológicas, Universidade Federal de Juiz de Fora, Rua José Lourenço Kelmer s/n - Campus Universitário São Pedro, Juiz de Fora, Minas Gerais, 36036900, Brazil

    Flavia de Paoli

  5. Departamento de Biofísica e Biometria, Instituto de Biologia Roberto Alcântara Gomes, Avenida 28 de Setembro 87 Vila Isabel, Rio de Janeiro, 20551030, Brazil

    Adenilson de Souza da Fonseca

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  1. Roberta da Silva Marciano
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  2. Luiz Philippe da Silva Sergio
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  3. Giovanni Augusto Castanheira Polignano
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  4. Giuseppe Antonio Presta
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  6. Mauro Geller
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  8. Flavia de Paoli
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  9. Adenilson de Souza da Fonseca
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Correspondence to Adenilson de Souza da Fonseca.

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da Silva Marciano, R., da Silva Sergio, L.P., Polignano, G.A.C. et al. Laser for treatment of aphthous ulcers on bacteria cultures and DNA. Photochem Photobiol Sci 11, 1476–1483 (2012). https://doi.org/10.1039/c2pp25027f

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