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Noninvasive fluorescence excitation spectroscopy during application of 5-aminolevulinic acid in vivo

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Abstract

The fluorescence of PpIX induced by topical application of 5-aminolevulinic acid (ALA) in normal mouse skin was studied noninvasively by means of a fibre optic probe. The fluorescence excitation spectrum of PpIX exhibits five distinct peaks at around 408, 510, 543, 583 and 633 nm under fluorescence monitoring at the second emission peak of PpIX (705 nm). The transmission of the excitation light is wavelength dependent: the long wavelength light (>600 nm) penetrates deeper into the tissues by a factor of 6 compared with the short wavelength light (<590 nm). Thus, the fluorescence excitation spectrum of PpIX measured on the surface of the skin can be used to estimate the depth of the penetration of topically applied ALA. The fluorescence excitation spectra calculated for the depth 1.1 mm obtained the best fit with the experimentally measured spectra after topical application of ALA.

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Authors and Affiliations

  1. Department of Biophysics, The Norwegian Radium Hospital, Oslo, Norway

    Petras Juzenas, Asta Juzeniene, Olav Kaalhus, Vladimir Iani & Johan Moan

  2. Laser Research Centre, Vilnius University, Vilnius, Lithuania

    Petras Juzenas

  3. Institute of Physics, Oslo University, Oslo, Norway

    Johan Moan

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  1. Petras Juzenas
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  2. Asta Juzeniene
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  3. Olav Kaalhus
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  4. Vladimir Iani
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  5. Johan Moan
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Juzenas, P., Juzeniene, A., Kaalhus, O. et al. Noninvasive fluorescence excitation spectroscopy during application of 5-aminolevulinic acid in vivo. Photochem Photobiol Sci 1, 745–748 (2002). https://doi.org/10.1039/b203459j

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