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CLL

Increased bone marrow angiogenesis in B cell chronic lymphocytic leukemia

Leukemia volume 14, pages 1414–1418 (2000)Cite this article

Abstract

Recent studies have shown that angiogenesis may be involved in the pathogenesis of hematopoietic malignancies, apart from its well-characterized role in the growth and metastasis of solid tumors. In this study, we quantified the degree of angiogenesis in B cell chronic lymphocytic leukemia (B-CLL) by measuring the microvessel density and hotspot density in bone marrow trephine biopsy sections with B-CLL involvement (n = 12) and compared it to normal bone marrow sections (n = 11). The B-CLL samples had a mean microvessel count/high power field (hpf) of 7.64 while the control samples had a mean microvessel count/hpf of 2.11 (P = 0.0001). The mean hotspot density in the B-CLL sections (14.83/hotspot) was also significantly higher (P = 0.0008) than the mean hotspot density in control bone marrow sections (7.09/hotspot). Both the microvessel density and hotspot density correlated positively with the clinical stage of the B-CLL patients. In a separate cohort of B-CLL patients, the median urine level of the angiogenic peptide, basic fibroblast growth factor (2216.5 pg/g, n = 14), was significantly higher (P = 0.0001) than the bFGF level in normal controls (1084 pg/g, n = 58). These results indicate that angiogenesis may be involved in the pathogenesis of B-CLL.

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Acknowledgements

We would like to thank Nancy Bone and Susan Connors for technical assistance, and Dr Judah Folkman for helpful suggestions and encouragement.

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Authors and Affiliations

  1. Northwestern University Medical School, Chicago, IL, USA

    AR Kini & LC Peterson

  2. Virginia Piper Cancer Institute, Minneapolis, MN, USA

    NE Kay

  3. Mayo Clinic, Rochester, MN, USA

    NE Kay

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  1. AR Kini
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  2. NE Kay
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Kini, A., Kay, N. & Peterson, L. Increased bone marrow angiogenesis in B cell chronic lymphocytic leukemia. Leukemia 14, 1414–1418 (2000). https://doi.org/10.1038/sj.leu.2401825

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