Abstract
Myeloid cell leukaemia-1 (Mcl-1) is an anti-apoptotic member of the Bcl-2 family that is elevated in a variety of tumour types including breast cancer. In breast tumours, increased Mcl-1 expression correlates with high tumour grade and poor patient survival. We have previously demonstrated that Her-2 levels correspond to increased Mcl-1 expression in breast tumours. Epidermal growth factor (EGF) receptor signalling is frequently deregulated in breast cancer and leads to increased proliferation and survival. Herein, we determined the critical downstream signals responsible for the EGF mediated increase of Mcl-1 and their role in cell survival. We found that both Mcl-1 mRNA and protein levels are rapidly induced upon stimulation with EGF. Promoter analysis revealed that an Elk-1 transcription factor-binding site is critical for EGF activation of the Mcl-1 promoter. Furthermore, we found that knockdown of Elk-1or inhibition of the Erk signalling pathway was sufficient to block EGF upregulation of Mcl-1 and EGF mediated cell survival. Using chromatin immunoprecipitation and biotin labelled probes of the Mcl-1 promoter, we found that Elk-1 and serum response factor are bound to the promoter after EGF stimulation. To determine whether Mcl-1 confers a survival advantage, we found that knockdown of Mcl-1 expression increased apoptosis whereas overexpression of Mcl-1 inhibited drug induced cell death. In human breast tumours, we found a correlation between phosphorylated Elk-1 and Mcl-1 protein levels. These results indicate that the EGF induced activation of Elk-1 is an important mediator of Mcl-1 expression and cell survival and therefore a potential therapeutic target in breast cancer.
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Introduction
Myeloid cell leukaemia-1 (Mcl-1) is an anti-apoptotic member of the Bcl-2 family of proteins that has a critical role in regulating the balance between survival and death signals (Yip and Reed, 2008). Anti-apoptotic Bcl-2 family members are frequently deregulated in human cancers and Mcl-1 is elevated in a variety of tumour types (Backus et al., 2001; Zhang et al., 2002; Song et al., 2005) including breast cancer, where it has been found to correlate with poor prognosis (Ding et al., 2007). Small molecule inhibitors targeting pro-survival Bcl-2 family members are currently in clinical trials (Vogler et al., 2009); however, due to inherent low affinities for Mcl-1, Mcl-1 overexpression was frequently found to mediate resistance (Oltersdorf et al., 2005; van Delft et al., 2006; Lin et al., 2007; Tse et al., 2008). More recently developed small molecule inhibitors that have a higher ability to disrupt Mcl-1 function have overcome resistance to other pan-Bcl-2 family inhibitors (Nguyen et al., 2007). This demonstrates the importance of Mcl-1 in the regulation of apoptosis.
The epidermal growth factor (EGF) receptor family is composed of four key membrane spanning receptors referred to as ErbB1-4 (Her-1-4). Of these receptors, ErbB1 (Her-1) and ErbB2 (Her-2) are most frequently overexpressed in human cancers (Klapper et al., 2000). In breast cancer, ErbB2 (Her-2) is overexpressed in roughly 20% of cases and correlates with poor prognosis (Singleton and Strickler 1992; Nanda 2007). Mcl-1 has previously been described in the literature as a downstream target of the EGF signalling pathway in oesophageal (Leu et al., 2000), colorectal (Schulze-Bergkamen et al., 2008) and lung (Song et al., 2005) cancer cell lines. We have evidence that suggests a similar response is seen in breast cancer and have found a correlation between Her-2 positive breast cancers and high levels of Mcl-1 expression (Henson et al., 2006). Although one report has demonstrated a correlation between phosphorylated Stat-3 and elevated Mcl-1 in breast tumours (Hsieh et al., 2005), the transcriptional mechanisms governing Mcl-1 expression in breast cancer have not been thoroughly investigated.
A number of studies exist describing the transcriptional regulation of the Mcl-1 gene in a diverse set of cell types. The Mek/Erk pathway has been implicated to govern Mcl-1 transcription in several cell line models (Boucher et al., 2000; Leu et al., 2000; Schubert and Duronio 2001). Two independent studies have demonstrated that Elk-1 and serum response factor (SRF), downstream of Erk activation, contribute to basal Mcl-1 expression levels as well as induction of transcription by treatment with 12-O-tetradecanoylphorbol-13-acetate in HEK 293, HeLa and Ml-1 cell lines (Townsend et al., 1999; Vickers et al., 2004). In other cell types, the PI3K/AKT pathway has been described as critical for Mcl-1 regulation (Wang et al., 1999; Kuo et al., 2001; Longo et al., 2008). Additionally, the transcription factor Stat-3 has been tied to Mcl-1 expression in cells of hematopoietic origin (Puthier et al., 1999; Liu et al., 2003). The precise signalling and transcriptional mechanisms regulating Mcl-1 in breast cancer cells remains unclear.
Herein, we determined that Erk activation of the transcription factor Elk-1 is critical for transcriptional regulation of Mcl-1 following EGF treatment and also has a significant role in EGF mediated cell survival in breast cancer.
Results
Stimulation of the EGF signalling pathway upregulates Mcl-1 protein and mRNA levels
Several studies have suggested that EGF signalling regulates transcription of Mcl-1 (Leu et al., 2000; Cetin et al., 2010). We have previously found a correlation between elevated Her-2 and Mcl-1 levels in breast tumours (Henson et al., 2006). To further assess whether Mcl-1 expression is modified by EGF receptor activation in breast cancer, we studied Mcl-1 protein and mRNA levels in MCF-7 and SK-BR-3 breast cancer cell lines following treatment with EGF. As shown in Figure 1a, both MCF-7 and SK-BR-3 cells demonstrated a marked elevation of Mcl-1 protein levels within 2 h of treatment and protein levels remained elevated after 8 h of treatment. As Mcl-1 has a short half-life (Akgul et al., 2000), rapid fluctuations in protein levels can occur in the absence of a change in relative transcription of the Mcl-1 gene. Therefore it was necessary to determine whether the observed changes were a result of elevated transcription or modification of protein stability. Mcl-1 mRNA levels were detected over a 120 min time course following stimulation with EGF by semi-quantitative real-time PCR. After 30 min of EGF treatment, the Mcl-1 mRNA level was increased by fourfold in MCF-7 cells and at 60 min EGF treatment in SK-BR-3 cells the mRNA level had increased nearly threefold. The mRNA levels peaked after 90 min of EGF stimulation at a 12-fold increase in MCF-7 cells and fourfold increase in SK-BR-3 cells. Control treated cells failed to demonstrate an increase in Mcl-1 mRNA levels over the same time course (dashed line, Figure 1b). This strongly suggests that EGF signalling elevates transcription of the Mcl-1 gene in breast cancer cells.
A small region of the Mcl-1 promoter containing a serum response element is necessary for EGF induced transcription
We set out to identify the key regulatory elements necessary for EGF induced transcription of Mcl-1 in a breast cancer cell line model. To determine the critical region of the Mcl-1 promoter, a 3974 bp fragment upstream of the translation start site was amplified by PCR and cloned into the PGL-3 luciferase reporter vector. A series of deletion mutants were generated to narrow down the region of interest. A small segment of the promoter, ∼300 bp upstream of the translation start site, was found to be sufficient for both basal and EGF induced expression of the luciferase reporter in MCF-7 cells (Figure 2a). Sequence alignment of the human and mouse Mcl-1 promoters showed a region of high identity within this fragment (Figure 2b). A series of 7 bp deletions were created within the 4 kb promoter reporter construct to identify potentially important cis-acting DNA elements. Specifically, high scoring putative transcription factor binding sites and the region of high identity with the mouse Mcl-1 promoter were targeted for deletion (Figure 2b). Transcription factor binding sites were identified using the TFSearch software (Heinemeyer et al., 1998). We found that deletion of two regions corresponding to a potential Elk-1 binding site (Del. 3) and SRF CarG box (Del. 6 and Del. 7) reduced EGF activation of the Mcl-1 promoter (Figure 2c). Both of these sites were within the region of high identity between the human and mouse promoters (Figure 2b). Additional transcription factor binding sites including an ATF2 consensus site (Del. 1) and putative nuclear factor (NF)-κB binding sites (data not shown) were deleted, but failed to significantly alter Mcl-1 promoter activity. The full-length Mcl-1 promoter (−3974) was used as a positive control. Similar results were obtained with SK-BR-3 cells (Supplementary Figure 1A and B).
Elk-1 is activated by stimulation with EGF and is essential for control of Mcl-1 protein levels
The Mek/Erk pathway is activated by EGF and has been shown to activate the transcription factor Elk-1 (Yordy and Muise-Helmericks, 2000). To determine whether EGF stimulation resulted in activation of Erk1/2 and Elk-1, MCF-7 and SK-BR-3 cells were treated with EGF over a 60 min time course. As shown in Figure 3a, Erk1/2 were phosphorylated within 5 min and phosphorylation decreased after 60 min of EGF treatment. Following similar kinetics, phosphorylation of Elk-1 at Ser383 was detected at 5 min following EGF treatment and decreased by 60 min. After 60 min, an increase in Mcl-1 protein was detected in MCF-7 cells. Control treatment failed to induce phosphorylation of these proteins over the same time course. Similar results were observed in SK-BR-3 cells (Figure 3a). Pre-treatment with U0126, a highly specific inhibitor of Mek1/2 (Favata et al., 1998) prevented both Erk1/2 and Elk-1 phosphorylation in MCF-7 and SK-BR-3 cells. This inhibition also prevented the elevation of Mcl-1 protein levels following EGF treatment (Figure 3b). The Erk inhibitor 3-(2-aminoethyl)-5-((4-ethoxyphenyl)methylene)-2,4-thiazolidinedione, previously published to prevent Elk-1 phosphorylation at Ser383 (Chen et al., 2006), also produced similar results (Figure 6c). Targeted knockdown of Elk-1 expression by transfection of a specific small interfering (si)RNA reduced both basal and EGF induced Mcl-1 levels substantially in both cell lines (Figure 3c). Knockdown of two other transcription factors, Stat-3 and NF-κB, failed to have an effect on basal or EGF induced levels of Mcl-1 (Supplementary Figure 2A). Unexpectedly, knockdown of SRF had no impact on basal or EGF induced Mcl-1 protein or mRNA levels (Supplementary Figure 2A/B). This data confirms the importance of Elk-1in transcriptional regulation of Mcl-1 in breast cancer cells.
Elk-1 and SRF bind to the Mcl-1 promoter
To assess whether the transcription factors Elk-1 and SRF bind to the Mcl-1 promoter, a chromatin immunoprecipitation (ChIP) experiment was performed with MCF-7 cells using primers that amplify a 163 bp region containing the putative Elk-1 and SRF binding sites identified in Figure 2c. ChIP was performed with Elk-1 and SRF antibodies along with two negative antibody controls and a no antibody control (beads alone). Elk-1 was detectable on the Mcl-1 promoter by ChIP before stimulation with EGF (Figures 4a and b). Under unstimulated conditions, SRF was only marginally detectable on the Mcl-1 promoter; however, within 10 min of adding EGF there was a significant elevation (85-fold enrichment) of SRF at the promoter, which was maintained after 30 min of stimulation (Figures 4a and b). This was followed by an elevation of Elk-1 at the 20 and 30 min time points. Isotype controls (ctrl 1 is an antibody against NF-κB, ctrl 2 is an antibody against Stat-3) failed to immunoprecipitate Mcl-1 promoter fragments and demonstrated fold enrichment values close to zero (Figures 4a and b). All of the antibodies failed to enrich a region of the third exon of the Mcl-1 gene (Figure 4b).
Further validation that Elk-1 and SRF bind to the region of interest was obtained by performing a streptavidin pull-down assay using a biotin-labelled 50 bp probe complementary to the region of interest (155–205 bp upstream of translation start). The Mcl-1 promoter specific probe was able to pull down both Elk-1 and SRF from EGF treated nuclear lysates (Figure 4c). The scrambled probe did not demonstrate observable binding and the presence of an excess of unlabelled probe successfully competed away the signal for both Elk-1 and SRF. Binding specificity was determined by using antibodies against two other transcription factors, NF-κB and Stat-3 (Figure 4c). The decrease in SRF binding observed following stimulation in the pull-down assay may be due to increased recruitment of SRF to the chromatin and therefore reduced availability of the protein in the assay.
Both EGF treatment and modulation of Mcl-1 expression impact the survival of SK-BR-3 cells
It has been established that EGF receptor activation elevates survival and resistance to apoptosis (Klapper et al., 2000). In Figure 5a, we confirmed that treatment of SK-BR-3 cells with EGF significantly reduced the levels of apoptosis induced by both etoposide and doxorubicin treatment. Protection was most evident at low drug concentrations wherein EGF reduced 5 μM etoposide induced cell death from 42 to 21% and 0.5 μM doxorubicin induced cell death from 32 to 12%. To assess the contribution of Mcl-1 to the survival of breast cancer cells we performed a targeted knockdown experiment with Mcl-1 specific small interfering (si)RNA. Twenty-four hours following siRNA transfection there was a substantially higher number of apoptotic cells (38%) with Mcl-1 knockdown as compared with untreated cells (9%) or transfection with a scrambled control siRNA (12%, Figure 5b). This implies that a minimal level of Mcl-1 expression is critical for cell survival in breast cancer cell lines. Overexpression of Mcl-1 by transfection of the Mcl-1 complementary DNA resulted in resistance to transfection toxicity (25%) and etoposide (45%) induced cell death as compared with the empty vector (40 and 55%, respectively, Figure 4c). These results demonstrate that Mcl-1 has an important role in cell survival.
EGF protects breast cancer cells from apoptosis through a mechanism that relies on signalling via the Mek/Erk pathway
As Mcl-1 knockdown alone was sufficient to induce apoptosis, we determined whether prevention of Mcl-1 upregulation would reverse the protective effects conferred by EGF pre-treatment. The Mek inhibitor U0126 completely prevents EGF induced upregulation of Mcl-1 protein levels (Figure 6a) and also results in a significant reduction of the protective effect of EGF (Figure 6b). In both the control and U0126 treated cells, etoposide induced nearly identical levels of apoptosis (39.4% in control and 41.4% in the U0126 pre-treated cells). Pre-treatment with EGF resulted in a reduction in the amount of apoptosis induced by etoposide from 39 to 19%. When the cells were also pre-treated with U0126 the EGF treatment only resulted in a reduction from 41 to 31% (Figure 6b).
To verify the result with U0126, the experiment was repeated using an Erk1/2 inhibitor (Figures 6c and d). The Erk1/2 inhibitor alone displayed slight toxicity that appeared to be enhanced in the presence of EGF. Despite the toxicity of the inhibitor, levels of apoptosis induced by etoposide were only 5% higher with the inhibitor as compared with that of the control (28.7 and 33.6%). Although EGF protection in the control samples was similar to that observed in Figure 6b (reduction from 28.7 to 12.8%), Erk inhibition completely reversed the protective effect conferred by EGF pre-treatment. In stark contrast to the control samples, co-treatment of etoposide with EGF in the presence of the Erk inhibitor showed higher levels of apoptosis compare with etoposide alone. These results indicate that Mek/Erk signalling leads to increased Mcl-1 expression and has a critical role in the EGF survival response in breast cancer cells.
Activated Elk-1 correlates with increased levels of Mcl-1 in breast tumour samples
Knowledge of the molecular pathways governing Mcl-1 expression allows for the development of rationale therapeutic approaches; however, without clinically relevant breast tumours the significance of these results will be diminished. To that end, we sought to determine if the signalling pathways regulating Mcl-1 expression in the MCF-7 and SK-BR-3 cell lines are correlated to Mcl-1 expression in human breast tumour samples. Before proceeding to a large-scale analysis, a pilot study was performed with 26 breast tumour samples from the Manitoba Breast Tumour Bank. Tumour sections were stained for immunofluorescence with antibodies directed against Mcl-1 and phosphorylated Elk-1. Antibodies were pre-validated for immunofluorescence on tumour sections by overexpression and knockdown experiments in MCF-7 cells (Supplementary Figure 3A and B). A panel of 26 breast tumour sections were stained with antibodies against Mcl-1 and phospho-Elk-1 and scored using a 0–3 point scoring system. In Supplementary Figure 4A, the data was analysed by plotting the scores as an XY scatter and performing a Spearman correlation test. We found a statistically significant positive correlation between phosphorylation of Elk-1 and Mcl-1 expression (rS=0.4303, P=0.0282) in the tested breast tumours. Representative images from the sampled tumours shown in Supplementary Figure 4b demonstrate that regions within an individual tissue section having high phospho-Elk-1 (green signal), also demonstrate strong staining for Mcl-1 (red signal). Conversely, regions with low phospho-Elk-1 levels tended to have corresponding low levels of Mcl-1.
Based upon the pilot study, we further investigated the relationship between activated Elk-1 and the expression level of Mcl-1 in a tissue microarray (TMA) provided by the Manitoba Breast Tumour Bank. The TMA consisted of 255 tumours that were previously assessed as ERα negative by a ligand binding assay. Each tumour was represented by two separate spots on the TMA. TMAs were immunostained with the Mcl-1 and phospho-Elk-1 antibodies previously demonstrated to be effective for immunofluorescence as well as antibodies directed against phospho-Erk1/2, ErbB1 and ErbB2. In Figures 7a and b, the tumours were separated into two groups based on the median Mcl-1 H-score of 120. Tumours with a Mcl-1 score of 120 or less were classified as ‘low Mcl-1’ and those with a score above the median were classified as ‘high Mcl-1’. A Mann–Whitney test was performed to determine if the median phospho-Elk-1 and phospho-Erk1/2 scores (indicated by the solid horizontal line) were significantly different between the two groups. The median phospho-Elk-1 score for the low-Mcl-1 category was 75.0 and the median score for the high Mcl-1 score was 140, a difference that was statistically significant (P<0.005). The median phospho-Erk1/2 scores were also significantly different with a score of 50.0 in the low-Mcl-1 subset and a score of 100.0 in the high subset. The phospho-Erk1/2 scores were also separated into two groups based on the median phospho-Elk-1 score. As Elk-1 is a substrate for Erk1/2 it was hypothesized that elevated levels of phospho-Erk1/2 would correlate with elevated levels of phospho-Elk-1. A significantly higher phospho-Erk1/2 score (150.0) was observed in tumours that demonstrated high levels of phospho-Elk-1 as compared with those that did not (50.0). In a similar manner the tumours were divided based on the median scores for ErbB1 and ErbB2. Increased expression of both receptors associated with a higher median Mcl-1 H-score (Figures 7d and e). This data supports the regulation of Mcl-1 protein expression by EGF receptors through the Mek/Erk pathways mediated by Elk-1 activation within breast tumours.
Discussion
Mcl-1 is unique amongst the pro-survival Bcl-2 family members in the degree to which its expression is tightly regulated and the multiple levels at which this occurs (Akgul 2009). Besides having its expression being regulated at the transcriptional level, Mcl-1 also contains a PEST domain that confers a very short protein half-life due to degradation via the proteasome pathway (Akgul et al., 2000). This property allows for tight regulation of Mcl-1 and protein levels fluctuate rapidly in response to the changing extracellular environment. Another degree of regulation exists via alternative splicing to generate a smaller protein that is an activator of apoptosis (Bingle et al., 2000). Mcl-1 is also subjected to multiple post-translational modifications that include cleavage by caspases or enhancement of stability through phosphorylation (Le Gouill et al., 2004). Mcl-1 is further regulated by the mir-29b microRNA (Mott et al., 2007). These studies indicate that Mcl-1 is a dynamically regulated protein, and our results demonstrate that transcriptional control is an important aspect of Mcl-1 expression in breast cancer.
Overexpression or mutation of the EGF receptors is seen in a variety of tumour types (Klapper et al., 2000). In breast cancer, the EGF receptor ErbB2/Her-2 is overexpressed in ∼20% of cases (Nanda 2007; Singleton and Strickler 1992). The ErbB1 receptor is also increased in breast cancer but increased gene transcription rather than gene amplification appears to be the primary reason for overexpression (Chrysogelos and Dickson, 1994). In contrast to normal tissue the expression level of ErbB1 in breast cancer can be increased by as much as 20-fold (Herbst, 2004). Although less studied, ErbB3 is also found to be increased in 19–29% of breast cancer cases and this increase, like that of ErbB1, is not primarily due to a change in gene copy number (Sithanandam and Anderson, 2008). Although the prognostic significance of ErbB3 in breast cancer is not as straightforward as for Her-2, a general trend towards disease progression and poorer outcome is reported (Sithanandam and Anderson, 2008).
Therapeutics that target the EGF receptors ErbB1 and ErbB2 such as Herceptin and Lapatinib have successful clinical applications in breast cancer (Johnston et al., 2006). Despite these advances, resistance to treatments has been a recurring theme (Nahta and Esteva, 2006). We have previously shown a correlation between Her-2 overexpression and elevated Mcl-1 protein expression. We have also demonstrated that Mcl-1 can contribute to cell survival and resistance against Herceptin (Henson et al., 2006). In this study we confirmed the relationship between EGF signalling and upregulation of Mcl-1 and identified that the Mek/Erk signalling pathway, acting ultimately through activation of Elk-1, is critical in regulating Mcl-1 expression in breast cancer cells. We have also taken the results obtained in breast cancer cell lines and used them to make accurate predictions in breast tumour samples, supporting the clinical relevance of the study. Furthermore, we have determined that Mek/Erk signalling is essential for the survival advantage conferred by EGF in our cell line models. Over activation of any of the downstream components of this pathway could contribute to resistance to EGF receptor targeted therapies through an upregulation of Mcl-1.
It has previously been established that Mcl-1 is overexpressed in breast tumours and correlates with poor prognosis (Ding et al., 2007). In this study, we have demonstrated that Mcl-1 is a downstream target of activated EGF receptor signalling and that overexpression of Mcl-1 confers resistance to drug induced apoptosis. We have also demonstrated that a minimal expression level of Mcl-1 is critical for survival of the SK-BR-3 breast cancer cell line. This data indicate that Mcl-1 may be an important target for breast cancer treatment. Several in vitro studies have demonstrated effectiveness of Bcl-2 family inhibitors against breast cancer (Martin et al., 2009; Witters et al., 2007). The Bcl-2 inhibitor ABT-737 has shown effectiveness in breast cancer cells alone or in combination with EGF receptor inhibitors (Witters et al., 2007). ABT-737 resistant cells show increased Mcl-1 expression indicating that targeted Mcl-1 expression could be an effective treatment (Nguyen et al., 2007; Vogler et al., 2009).
We have shown that the transcription factor Elk-1 is an important regulator of Mcl-1 expression. This supports studies describing Mcl-1 transcriptional regulation by tetradecanoylphorbol-13-acetate in HEK 293, HeLa and Ml-1 cell lines (Boros et al., 2009; Townsend et al., 1999; Vickers et al., 2004). Although we were able to validate the importance of Elk-1 in controlling Mcl-1 expression we found that knockdown of SRF had little or no effect on basal or induced Mcl-1 expression (Supplementary Figure 2) Whether this is due to insufficient knockdown, recruitment of other co-activators in the absence of SRF or Elk-1 acting independently is yet to be determined. Although many additional transcription factor binding sites were found within the Mcl-1 promoter such as Stat-3 and NF-κB, two transcription factors that may have a role in Mcl-1 regulation in other cell types, it appears that these sites are not critical for the EGF induced regulation of Mcl-1 in these breast cancer cell lines (Boucher et al., 2000; Henson et al., 2006; Liu et al., 2003; Tsutsui et al., 2009). Furthermore, knockdown of both Stat-3 and NF-κB had no observable effect on basal or EGF induced Mcl-1 protein levels (Supplementary Figure 2A). Taken together, this suggests that activation of Elk-1 is critical for upregulation of Mcl-1 expression at least in breast cancer cells.
The Mek/Erk signalling pathway has an important role in breast cancer progression (Sivaraman et al., 1997; Mueller et al., 2000). Although Ras mutations are infrequently observed in breast cancer, elevated activation of the protein is observed in a substantial proportion of tumours (Sivaraman et al., 1997; von Lintig et al., 2000). The basal-like subset of breast tumours has been identified as particularly susceptible to Mek inhibition (Mirzoeva et al., 2009). Furthermore, the Mek inhibitor PD0325901 has been found to be effective as a single agent as well as in combination with inhibitors of PI3K in murine xenograft models of basal-like breast cancer (Hoeflich et al., 2009). Recently, the use of Mek inhibitors has demonstrated success in overcoming resistance to the EGF receptor inhibitor lapatinib (Sambade et al., 2009; Zoppoli et al., 2010). Our results point to the possibility that inhibitors of the Mek/Erk signalling cascade can be effective at blocking the expression of Mcl-1 in breast cancer. Our data also suggest that Mcl-1 is an important downstream mediator of the survival benefits conferred by overactivation of this signalling network. Inhibition of the transcription factor Elk-1 could be a further approach to block the overexpression of Mcl-1; however, small molecule inhibitors to Elk-1 are currently unavailable. Nevertheless, alterations in the Mcl-1 transcriptional regulation could be a viable strategy to sensitize breast cancer cells to EGF receptor inhibitors and Bcl-2 inhibitors.
Materials and methods
Cell culture and reagents
The human breast adenocarcinoma cell lines MCF-7 and SK-BR-3 were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA) in October of 2008 and December of 2009, respectively. Cell identity was confirmed by the ATCC by short tandem repeat analysis. Cells were maintained in a humidified 5% CO2 incubator at 37 °C and were passaged twice weekly. Cells were passaged no more than 20 times. MCF-7 cells were maintained in Dulbecco's modified Eagle's medium (Invitrogen, Burlington, ON Canada) supplemented with 10% fetal calf serum (Fisher Scientific, Pittsburgh PA, USA) and 100 units/ml penicillin and 100 μg/ml streptomycin (Invitrogen). SK-BR-3 cells were maintained in McCoy's 5A medium (Invitrogen) with identical supplements. Recombinant human EGF was obtained from Sigma-Aldrich (Oakville, ON, Canada) and dissolved in 10 mM acetic acid with 0.1% bovine serum albumin. For all experiments, EGF was added to the media at a final concentration of 1 μg/ml. Etoposide and doxorubicin (Sigma-Aldrich) were dissolved in dimethyl sulfoxide at a stock concentration of 50 mM and stored at −20 °C. The MEK inhibitor U0126 (Promega, Madison, WI, USA) was dissolved in dimethyl sulfoxide at a concentration of 10 mM and stored at −20 °C. The Erk inhibitor 3-(2-aminoethyl)-5-((4-ethoxyphenyl) methylene)-2,4-thiazolidinedione (EMD Chemicals, Mississauga, ON, Canada) was dissolved in dimethyl sulfoxide at a concentration of 10 mg/ml and stored at −20 °C. The following antibodies were used: rabbit anti-Mcl-1 (M8434 Sigma-Aldrich), rabbit anti-Elk-1 (ab32106 Abcam, Cambridge, MA, USA), mouse anti-SRF (MAB4369 Millipore, Billerica, MA, USA), rabbit anti-phospho-Elk-1 (Ser383) (9181, Cell Signaling, Boston, MA, USA), mouse anti-phospho-P44/42 MAPK (Thr202/Tyr204) (9106, Cell Signaling), rabbit anti-P44/42 MAPK (9102, Cell Signaling), rabbit anti-NF-κB p65 (ab7970 Abcam), mouse anti-α-tubulin (T6074 Sigma-Aldrich), rabbit-anti-Stat-3 (9132, Cell Signaling), rabbit anti-Her-2 (Dako, Mississauga, ON, Canada) and mouse anti-EGFR (Ventana, Tucson AZ, USA).
Western blotting
All whole-cell lysates were prepared with RIPA buffer (50 mM Tris pH 8.0, 150 mM NaCl, 2 mM EDTA, 1% nonident P-40, 0.5% sodium deoxycholate, 0.1% SDS) supplemented with protease inhibitors (Complete mini, Roche, Laval, QC, Canada) and phosphatase inhibitors (phosphatase inhibitor cocktail 1 and 2, Sigma-Aldrich). Equal amounts of protein were resolved by SDS/polyacrylamide gel electrophoresis and transferred to polyvinyl difluoride membranes. Membranes were blocked in Tris buffered saline containing 0.1% Tween-20 (Tris-buffered saline Tween-20) and 5% skim milk powder. Primary antibodies were incubated overnight at 4 °C in 5% milk Tris-buffered saline tween-20. Following incubation, membranes were washed threefold in Tris-buffered saline tween-20 and then incubated with the appropriate secondary antibody conjugated to horse radish peroxidise for 1 hour at room temperature in 5% milk tris-buffered saline tween-20. Proteins were visualized on Hyperfilm ECL by enhanced chemiluminescence (GE Healthcare, Piscataway NJ, USA).
RNA isolation and real-time (RT)–PCR
Total RNA was isolated using the Qiagen RNeasy Plus mini kit according to the manufacturer's protocol and RNA concentrations were quantified by measurement of absorbance at 260 nm with a spectraphotometer (SpectraMax M5, Molecular Devices, Sunnydale, CA, USA). In all 100 ng total RNA was used as template for the real-time PCR. One-step RT–PCR was performed using the iScript One-step RT–PCR kit (Bio-Rad, Hercules, CA, USA) and cycling and data collection were performed on an iCycler thermal cycler (Bio-Rad) using the supplied software (iCycler IQ ver 3.1, Bio-Rad). The following primers specific to the Mcl-1 mRNA were used: forward: 5′-GCCAAGGACACAAAGCCAAT-3′, reverse: 5′-AACTCCACAAACCCATCCCA-3′. The following primers specific to the housekeeping gene cyclophilin were used to standardize results: forward: 5′-GCTGCGTTCATTCCTTTG-3′, reverse: 5′-CTCCTGGGTCTCTGCTTTG-3′. The following cycling conditions were used: 50 °C for 10 min followed by 95 °C for 5 min, then 40 cycles of 95 °C for 10 s followed by 55 °C for 30 s (data collection step). Primer specificity was confirmed by visualizing DNA on an agarose gel following PCR.
Mcl-1 promoter constructs and luciferase assays
The Mcl-1 promoter was amplified by PCR from a BAC clone containing the appropriate region of chromosome 1 (RP11-54A4, Invitrogen). Primers were designed to amplify the 5′ promoter region (3974 bp downstream of the translation start site), as well as to generate seven large deletions from the 5′ end. Promoter fragments were cloned into the PGL3 luciferase reporter vector (Promega). A 7-bp deletions were introduced into the 3974 bp fragment of the Mcl-1 promoter using the Quikchange II site directed mutagenesis kit according to the manufacturer's protocol (Stratagene, Santa Clara, CA, USA). The reporter constructs were transfected into MCF-7 and SK-BR-3 cells using GenePorter 2 transfection reagent (Genlantis, San Diego, CA, USA) along with a plasmid containing the β-galactosidase complementary DNA to standardize results. Twenty-four hours after transfection, the cells were serum starved for an additional 24 h and then treated with EGF or vehicle control. After 6 h treatment, cells were lysed in 200 μl reporter lysis buffer (Promega) and luciferase activity was measured in 20 μl lysate on an LMAX luminometer (Molecular devices) with 100 μl luciferase assay substrate (Promega). The β-galactosidase activity was assessed by combining 50 μl lysate with 50 μl 2X β-gal buffer (200 mM sodium phosphate pH 7.3, 2 mM MgCl2, 100 mM β-mercaptoethanol, 1.33 mg/ml ONPG) and measuring the absorbance at 450 nm.
ChIP
ChIP was performed following EGF stimulation according a previously published protocol (Spencer et al., 2003). Following immunoprecipitation, and RNAse/proteinase K digestion of samples, ChIP DNA was isolated using the QiaQuick PCR purification kit (Qiagen, Mississauga, ON, Canada) and DNA concentration was determined using the PicoGreen dsDNA quantitation assay (Invitrogen). In all 0.1 ng ChIP DNA was amplified by RT-PCR using primers specific to the Mcl-1 promoter (forward: 5′-TAGGTGCCGTGCGCAACCCT-3′, reverse: 5′-ACTGGAAGGAAGCGGAAGTGAGAA-3′) or the last exon of the Mcl-1 gene (forward: 5′-TGTTGCTGGAGTAGGAGCTGGTTT-3′, reverse: 5′-GCCATAATCCTCTTGCCACTTGCT-3′). To obtain fold enrichment values, the cT value of each ChIP sample was compared with the cT value of 0.1 ng input DNA.
Streptavidin pull-down assay
To assess transcription factor binding to an Mcl-1 promoter specific probe, a streptavidin pull-down assay was performed with biotin labelled probes specific to the Mcl-1 specific promoter. The probe sequence is as follows: 5′-CAACCCTCCGGAAGCTGCCGCCCCTTTCCCCTTTTATGGGAATACTTTTT-3′. Following treatment with EGF for the indicated times, nuclear extraction was performed on 20 × 106 cells per time point. Nuclear extracts were pre-cleared with 50 μl streptavidin agarose beads (Invitrogen) for 30 min at 4 °C with rotation. Following pre-clearance, a binding reaction was prepared that contained 500 μg nuclear extract, 50 ng/μl poly dI-dC, 1/5 volume 5 × binding buffer (50 mM Tris pH 7.5, 250 mM KCl, 5 mM dithiothreitol) and 100 nM biotin labelled probe. Binding reactions were incubated for 30 min at room temperature at which point 50 μl streptavidin-agarose beads were added. Following a 30 min incubation, beads were spun down at 3000 r.p.m. for 1 min and washed 3 × 5 min in phosphate-buffered saline. After washing, beads were resuspended in 50 μl 2X SDS loading dye, boiled for 5 min and the isolated proteins were separated by SDS/polyacrylamide gel electrophoresis and western blot was performed to assess transcription factor binding.
siRNA transfections
The siRNA transfections were carried out using the Nucleofector (Amaxa, Walkersville, MD, USA) electroporation device according to the cell line specific protocols provided by the manufacturer. In all 30 pmol total siRNA was transfected per sample and all experiments were performed 48 h following transfection. Pre-validated siRNA duplexes were obtained from Invitrogen. The following siRNAs were used: Mcl-1—5′-GAAAGUAUCACAGACGUUCUCGUAA-3′, Elk-1—5′-GCGGCCAGAAGUUCGUCUACAAGUU-3′, SRF—5′GCUACACGACCUUCAGCAAGAGGAA-3′ Stat-3— 5′GCAGUUUCUUCAGAGCAGGUAUCUU-3′, NF-κB—sequence unavailable: product #29410 (Santa Cruz Biotechnology, Santa Cruz, CA, USA).
Assessment of apoptosis
Analysis of apoptosis was performed by quantification of the sub-G1 peak by flow cytometry using a BD FACScalibur flow cytometer (BD Biosciences, Mississauga, ON, Canada) according to a previously published protocol (Riccardi and Nicoletti, 2006).
Immunostaining of paraffin embedded tissue sections and tumour microarrays
Tumour tissue sections were acquired from the Manitoba Breast Tumour Bank. Immunofluorescence was performed as previously published (Henson et al., 2006). Antibodies were validated by immunofluorescence performed on cells grown on coverslips with and without knockdown of the protein of interest by siRNA. TMAs were acquired from the Manitoba Breast Tumour Bank, operating under the approval of the Faculty of Medicine, University of Manitoba, Research Ethics Board (Watson et al., 1996). The details regarding TMA preparation and the protocol for immunohistochemistry staining and development has been described previously (Skliris et al., 2008). TMAs were scored using the H-score method by two blinded independent observers under the supervision of a pathologist at CancerCare Manitoba.
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Acknowledgements
The work is supported by a grant from the Canadian Breast Cancer Foundation, Prairie chapter. Evan Booy is supported by the Canadian Institutes for Health Research (CIHR) CGS Doctoral award. Dr Gibson is a Manitoba Research Chair. The Manitoba Tumour Bank is a member of the Canadian Tumour Repository Network (CTRNet) and is supported by a grant from the CIHR
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Booy, E., Henson, E. & Gibson, S. Epidermal growth factor regulates Mcl-1 expression through the MAPK-Elk-1 signalling pathway contributing to cell survival in breast cancer. Oncogene 30, 2367–2378 (2011). https://doi.org/10.1038/onc.2010.616
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DOI: https://doi.org/10.1038/onc.2010.616
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