Abstract
The product of the breast cancer-1 gene, BRCA1, plays a crucial part in the DNA damage response through its interactions with many proteins, including BACH1, CtIP and RAP80. Here we identify a coiled-coil domain–containing protein, CCDC98, as a BRCA1-interacting protein. CCDC98 colocalizes with BRCA1 and is required for the formation of BRCA1 foci in response to ionizing radiation. Moreover, like BRCA1, CCDC98 has a role in radiation sensitivity and damage-induced G2/M checkpoint control. Together, these results suggest that CCDC98 is a mediator of BRCA1 function involved in the mammalian DNA damage response.
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Acknowledgements
We thank members of the Chen laboratory for helpful discussions and technical support. This work was supported in part by grants from the US National Institute of Health (RO1CA089239 to J.C.) and the US Department of Defense breast cancer Era of Hope Scholar Award (W81XWH-05-1-0470 to J.C.).
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H.K., J.H. and J.C. designed experiments and interpreted the data; H.K. and J.H. performed all experiments; H.K. and J.C. prepared the manuscript.
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Kim, H., Huang, J. & Chen, J. CCDC98 is a BRCA1-BRCT domain–binding protein involved in the DNA damage response. Nat Struct Mol Biol 14, 710–715 (2007). https://doi.org/10.1038/nsmb1277
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DOI: https://doi.org/10.1038/nsmb1277
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