Abstract
Wnt signaling defines the colonic epithelial progenitor cell phenotype1, and mutations in the gene adenomatous polyposis coli (APC) that activate the Wnt pathway cause the familial adenomatous polyposis coli (FAP) syndrome and most sporadic colon cancers2. The mechanisms that regulate the transition of epithelial precursor cells into their differentiated derivatives are poorly characterized. We report that Indian hedgehog (Ihh) is expressed by mature colonocytes and regulates their differentiation in vitro and in vivo. Hedgehog (Hh) signaling restricts the expression of Wnt targets to the base of the colonic crypt in vivo, and transfection of Ihh into colon cancer cells leads to a downregulation of both components of the nuclear TCF4–β-catenin complex and abrogates endogenous Wnt signaling in vitro. In turn, expression of Ihh is downregulated in polyps of individuals with FAP and expression of doxycycline-inducible dominant negative TCF4 (dnTCF4) restores Ihh expression in APC mutant DLD-1 colon cancer cells. These data identify a new Wnt-Hh axis in colonic epithelial renewal.
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Terminally differentiated absorptive colonocytes produce Ihh mRNA and Ihh protein (Fig. 1a–f). The epithelium of Ihh+/+ mouse embryos consists of a monolayer of polarized epithelial cells that is organized into crypts at 16.5 days post coitum (d.p.c.; Fig. 1g,i). In contrast, the colonic epithelium of Ihh−/− embryos3 is a multilayer and does not organize into crypts (Fig. 1h,j). Whereas some epithelial cells in the Ihh+/+ controls differentiate by 16.5 d.p.c. and are negative for the proliferation marker PCNA (Fig. 1k), all epithelial cells in Ihh−/− colons are PCNA-positive at 16.5 d.p.c. (Fig. 1l). These results indicate that Ihh may have a role in colonic epithelial differentiation.
As Ihh−/− mice die before birth, we treated adult rats with the Hh signaling inhibitor cyclopamine to study the role of Hh signaling in adult colonic epithelial homeostasis. This plant steroidal alkaloid inhibits the cellular response to Hh signaling by directly binding to the Smo receptor4. The cyclopamine treatment induced a marked histological change in the colonic enterocytes of all treated rats: the luminal end of the crypt had an enlarged nucleus and large cytoplasm in contrast to the slender nucleus and cytoplasm of terminally differentiated enterocytes of controls (Fig. 1m–p). We used three molecular markers of colonic epithelial differentiation, Villin, a cytoskeletal protein that is specific for microvilli, the brush border enzyme carbonic anhydrase (CA) IV and the goblet cell marker intestinal trefoil factor (ITF), to characterize the maturation state of the enterocytes in the treated rats. Enterocytes in controls had a uniform Villin localization to the brush border (Fig. 1q). In contrast, the enterocytes in cyclopamine-treated rats showed heterogeneous Villin localization with loss of Villin expression at the brush border and redistribution of Villin to the cytoplasm (Fig. 1r). Whereas the colonic enterocytes of control rats highly expressed CA IV, the enterocytes in cyclopamine-treated rats were mostly CA IV–negative (Fig. 1s,t). In control rats, we found ITF staining mainly in goblet cells and on the brush border of normal enterocytes (Fig. 1u,w), as previously described by others5,6. Notably, the abnormal enterocytes in cyclopamine-treated rats showed strong immunoreactivity for ITF, indicating that they inappropriately express a marker of the goblet cell lineage (Fig. 1v,x).
To confirm the role of Hh signaling in the maturation of colonic enterocytes, we used the HT-29 colon carcinoma differentiation model. HT-29 cells start to polarize and form apical microvilli when treated with the short-chain fatty acid butyrate7. We used two different markers of this differentiation process: Villin8 and the cell cycle inhibitor Cip-1 (also called p21 and Waf1), which has an essential role in growth inhibition during colonic epithelial differentiation9. Ihh protein was induced in HT-29 cells on treatment with butyrate (Fig. 2a). Treatment of HT-29 cultures with cyclopamine substantially reduced butyrate-mediated induction of Cip-1 and Villin (Fig. 2b,c). Expression of Cip-1 and Villin was induced with recombinant N-terminal Shh peptide (Fig. 2d), which is 91% identical to the corresponding Ihh peptide, binds Ptc with the same affinity and induces the same biological response10. These results indicate the Hh signaling is both necessary and sufficient for HT-29 cell differentiation. Thus, we concluded that Hh signaling regulates colonic epithelial differentiation in vivo and in vitro.
We examined the expression of several proteins that possibly interact with Hh signaling. The Hh binding receptor Ptc was broadly expressed in the epithelial cells along the crypt axis and on mesenchymal cells (Fig. 3a). Ihh may directly affect a wide range of target cells in both the epithelium and the mesenchyme. During morphogenesis of the fruit fly gut, the Wnt target gene Engrailed is important in the specification of the large intestine11. In the adult colon, Engrailed-1 is expressed in epithelial cells along the entire crypt axis, with highest expression at its base (Fig. 3b). Engrailed-1 accumulated in the superficial enterocytes in cyclopamine-treated rats (Fig. 3c,d,i,j). The vertebrate Dpp homologs BMP2 and BMP4 are coexpressed with Hh genes during development12. We detected an epithelial gradient of BMP4 expression from the base to the tips of the crypts (Fig. 3e and ref. 1). In the adult colon, BMP2 is expressed by the differentiated colonocytes13. Hh inhibition induced higher expression of BMP4 but had no effect on the expression of BMP2 (Fig. 3i,j). Immunohistochemistry showed loss of the BMP4 expression gradient in the epithelium of cyclopamine-treated rats (Fig. 3e–j). Thus, we concluded that Hh signaling restricts expression of Engrailed-1 and BMP4 to the precursor cell compartment at the base of the colonic crypt.
Cyclin D1 was upregulated in cyclopamine-treated rats, and precursor cell proliferation increased 49% (Fig. 3i,k).Engrailed-1, BMP4 and Cyclin D1 are established Wnt targets. Because they were all upregulated in response to cyclopamine treatment in vivo, Ihh signaling could negatively regulate β-catenin–TCF signaling and restrict it to the crypt. Using the TOPflash-FOPflash reporter assay for β-catenin–TCF signaling activity14, we showed that Ihh could completely abrogate endogenous β-catenin–TCF signaling in DLD-1 colon cancer cells (Fig. 4a). This shutdown of β-catenin–TCF signaling correlated with downregulation of Wnt targets in DLD-1 cells (Fig. 4b). We observed no difference in the total cellular levels of β-catenin (Fig. 4b). However, we observed an almost complete downregulation of nuclear TCF4 expression and low levels of nuclear β-catenin in nuclear extracts of Ihh-transfected cells but not in control cultures transfected with a control vector (Fig. 4c). Thus, we conclude that Hh signaling restricts Wnt targets Engrailed-1 and BMP4 to the colonic precursor cell compartment in vivo and accordingly represses Wnt signaling in colon cancer cells in vitro.
Because Hh signaling negatively regulated Wnt signaling in the colonic epithelium, we were interested to see whether Wnt signaling affected the expression or activity of Ihh. Mutations in the gene APC initiate the development of colorectal cancer by constitutively activating the Wnt pathway. We examined Ihh expression in sporadic colonic adenomatous polyps and polyps of individuals with FAP (Fig. 5). In sporadic adenomatous polyps (n = 10), Ihh staining was lost from the dysplastic epithelial cells (Fig. 5a–c). In contrast, we found normal Ihh staining in the morphologically normal tissue present in seven of these specimens and resection specimens of normal colonic tissue (n = 3). To study early lesions of the adenoma-carcinoma sequence, we examined 12 resection specimens of six different individuals with FAP. Multiple very small adenomas can be found in these specimens owing to the frequent inactivation of both APC alleles in the affected individuals. As in sporadic adenomas, Ihh expression was lost in the dysplastic epithelial cells in adenomas of individuals with FAP (data not shown). Notably, in two different individuals with FAP we found areas with complete loss of Ihh staining in the epithelium of a few morphologically normal adjacent crypts (Fig. 5d,f). β-catenin staining of consecutive sections (Fig. 5e,g,h) showed loss of membrane staining and accumulation of β-catenin precipitates at the basolateral membrane in the same area. This aberrant localization of β-catenin corresponds to that previously described in human aberrant crypt foci, the earliest morphologically recognizable lesions of APC mutants15. These results suggest that Ihh expression is downregulated in response to constitutive β-catenin–TCF signaling. We therefore examined Ihh expression in APC mutant DLD-1 cells that carry doxycycline-inducible dnTCF4 (ref. 1). We observed rapid induction of Ihh expression on inhibition of β-catenin–TCF signaling in these colon cancer cells (Fig. 5i), indicating that Ihh expression was negatively regulated by β-catenin–TCF signaling and that the APC mutation caused loss of Ihh expression in colonic polyps.
Blocking Hh signaling increased colonic epithelial cell proliferation, in contrast with the recent observation that cell growth of upper digestive tract tumors depends on Hh signaling16,17. Instead of acting on precursor cells, Hh signaling in the adult colon seems to act mainly on differentiating enterocytes, and the effects we observed on precursor cell proliferation may therefore be indirect. Our data corroborate previous findings17 that there was no Hh pathway activity in colon cancer cell lines. Our studies suggest that Hh pathway activity is lost from colon cancer cells owing to the activation of Wnt signaling in these cells. Notably, the redistribution of Villin from the apical membrane to the cytoplasm, loss of expression of brush border enzymes and inappropriate induction of ITF expression in the enterocyte lineage that we observed in cyclopamine-treated rats are all aspects of loss of differentiation that are also observed during colorectal carcinogenesis6,18. Thus, the loss of Ihh expression secondary to the Wnt-activating APC mutation may have a role in the development of dysplasia in response to the APC mutation.
Taken together, our in vivo and in vitro data suggest that Ihh is an important extrinsic signal for a differentiating colonic enterocyte and that it restricts β-catenin–TCF signaling to the precursor cell compartment at the base of the colonic crypt. We show that Ihh expression, in turn, is negatively regulated by Wnt signaling in vivo and in vitro.
Methods
Antibodies.
We used the following antibodies, with dilutions for immunohistochemistry shown first, followed by those used for immunoblotting. Antibodies to BMP2 (clone 355; 1:1,000; 1:2,000) and to BMP4 (clone 757; 1:500; 1:2,000) were from R&D Systems. Antibodies to Shh, which recognizes the Shh precursor protein19 (N-19; 1:200; 1:2,000), to Hh (I-19; 1:50; 1:500), to Ihh (C-15; 1:50, 1:500), to Ptc (C-20, 1:50 for immunohistochemistry), to Villin (C-19; 1:1,000 for immunoblotting) and to β-actin (I-19; 1:5,000 for immunoblotting) were all from Santa Cruz. Antibody to Dhh (AVARP 7048, 1:50 for immunohistochemistry) was from Aviva. Antibody to Engrailed-1, developed in T.M. Jessell's laboratory (Columbia University, New York, New York, USA), was obtained from the Developmental Studies Hybridoma Bank. Antibodies to β-catenin (clone 14, 1:1,000 for immunohistochemistry) and to Cip-1 (clone 70, 1:5,000 for immunoblotting) were from Transduction Laboratories. Antibody to Cyclin D1 (clone DCS6, 1:1,000 for immunoblotting) was from Neomarkers. Antibodies to c-Met (clone DO-24, 1:2,000 for immunoblotting) and to TCF4 (clone 6H5-3, 1:500 for immunoblotting) were from Upstate Biotechnology. Rabbit polyclonal antibodies to Ptc (1:200 for immunohistochemistry), to ITF (1:500 for immunohistochemistry) and to CA IV (1:2,000 for immunohistochemistry) were gifts from R. Töftgard (Karolinska Institute, Sweden), D.K. Podolsky (Massachusetts General Hospital, Boston, Massachusetts, USA) and A. Waheed (St. Louis University School of Medicine, St. Louis, Missouri, USA), respectively. We confirmed the specificity of all antibodies used in immunohistochemistry on immunoblot and in experiments using the appropriate Ig controls. Secondary antibodies used were all from Dako.
Immunohistochemistry and in situ hybridization.
We obtained formalin-fixed paraffin-embedded human resection specimens of normal colonic mucosa, sporadic adenomas and individuals with FAP from the archives of the pathology department of the Academic Medical Center. We carried out immunohistochemical analysis as described19. We carried out control immunohistochemical experiments leaving out the primary antibody or with control Ig. We carried out in situ hybridization on paraffin sections as described20 using digoxigenin-labeled mRNA probes for human IHH and SHH, a gift from C. J. Tabin (Harvard Medical School, Boston, Massachusetts, USA).
Immunoblot.
We dissected the distal half of the rat colon along the longitudinal axis and homogenized and processed one half for western blotting as described19.
Cyclopamine treatment.
We treated 8-week-old female Wistar rats (n = 7) with daily intraperitoneal injections of 1 mg per kg body weight of the Hh inhibitor cyclopamine complexed with 2-hydroxypropyl-β-cyclodextrin (Sigma) for 14 d as described19. Control rats (n = 7) received solvent only. The experiment was approved by the animal ethics review board of the University of Amsterdam.
Cell culture.
We cultured HT 29 and DLD-1 colon cancer cell lines in the presence of 10% fetal calf serum and 4.5 mM glucose (GIBCO). The TOPflash-FOPflash TCF reporter constructs, a β-catenin expression construct and DLD-1 cells carrying a doxycycline-inducible dnTCF4 (cultured as described21) were gifts from H. Clevers (Hubrecht Laboratorium, the Netherlands). We transiently transfected DLD-1 cells with lipofectamine plus reagent (Invitrogen) according to the manufacturer's instructions. Luciferase activity was determined 24 h after transfection with a TOPCount microplate scintillation counter (Packard). The full-length Ihh expression construct was a gift from M. Hammerschmidt (Max-Planck Institute for Immunobiology, Germany). We carried out HT-29 cell experiments in six-well plates at the moment the cells reached confluence. We used butyrate (Sigma) at the indicated concentration and cyclopamine at 2 μg ml−1. To isolate nuclear extracts, we washed cells in ice-cold phosphate-buffered saline and scraped them into 200 μl of ice-cold lysis buffer (20 mM HEPES, pH 7.9; 1.5 mM MgCl2; 10 mM KCl; 0.5 dithiothreitol; 0.5 mM Pefabloc, 0.5 μg ml−1 leupeptin; 0.5 μg ml−1 aprotinin). We passed the lysate through a 27-gauge needle ten times. After centrifugation at 600g, we washed the resultant pellets (nuclear fraction) again in lysis buffer and resuspended them in sample buffer. We diluted the supernatants in sample buffer to yield the cytosolic fraction.
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Acknowledgements
We thank H. Clevers, M. Hammerschmidt, C. Tabin, D.K Podolsky, A. Waheed and R. Töftgard for reagents; A. Vortkamp for colons of Ihh−/− mice; and J. Daalhuisen for technical assistance.
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van den Brink, G., Bleuming, S., Hardwick, J. et al. Indian Hedgehog is an antagonist of Wnt signaling in colonic epithelial cell differentiation. Nat Genet 36, 277–282 (2004). https://doi.org/10.1038/ng1304
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DOI: https://doi.org/10.1038/ng1304
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