Abstract
THE T-cell receptor is necessary and sufficient for recognition of peptides presented by major histocompatibility complex molecules1,2. Other adhesion molecules, like CD4 or CDS, play an auxiliary role in antigen recognition by T cells3,4. Here we analyse T-cell receptor (TCR) binding using a soluble rather than a cell-bound receptor molecule. A TCR-immunoglobulin chimaera is constructed with the variable and the first constant regions of both the TCR α- andβ-chains linked to the immunoglobulin light-chain constant regions. This soluble TCR is expressed, assembled and secreted as an αβ heterodimer by a myeloma cell line transfected with the recombinant genes. Furthermore, the soluble TCR is biologically active: it specifically inhibits antigen-dependent activation of the relevant T-cell clones and thus discriminates between proper and irrelevant peptides presented by major histocompatibility complex molecules.
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References
Yagüe, J. et al. Cell 42, 81–87 (1985).
Dembic, Z. et al. Nature 320, 232–238 (1986).
Dembic, Z. et al. Nature 326, 510–511 (1987).
Gay, D. et al. Nature 328, 626–629 (1987).
Hackett, C. J. et al. J. exp. Med. 158, 294–302 (1983).
Taylor, A. H., Haberman, A. M., Gerhard, W. & Caton, A. J. J. exp. Med 172, 1643–1651 (1990).
Kappler, J. W. et al. in Regulation of the Immune Systems (eds Cantor, H., Chess, L. & Sercary, E.) 377–388 (Liss, New York, 1984).
Staerz, V. D. & Bevan, M. J. in Molecular Biology of the Immune System (eds Streilein, J. W. et al.) 61–64 (Cambridge University Press, 1985).
Haberman, A. M., Moller, C., McCreedy, D. & Gerhard, W. U. J. Immun. 145, 3087–3094 (1990).
Traunecker, K., Dolder, B., Oliveri, F. & Karjalainen, K. Immun. Today 10, 29–32 (1989).
Traunecker, A., Oliveri, F. & Karjalainen, K. Trends Biotech. 9, 109–113 (1991).
Traunecker, A., Dolder, B. & Karjalainen, K. Eur. J. Immun. 16, 851–854 (1986).
Davis, M. M. et al. Cold Spring Harb. Symp. quant. Biol. LIV 119–128 (1989).
Walker, E. N., Warner, N. L., Chestnut, R., Kappler, J. & Marrack, P. J. Immun. 128, 2164–2170 (1982).
Ozato, K., Mayer, N. & Sachs, D. H. J. Immun. 124, 533–540 (1980).
Shimonkevitz, R., Kappler, J., Marrack, P. & Grey, H. J. exp. Med. 158, 303–316 (1983).
Bhattacharya, A., Dorf, M. E. & Springer, T. A. J. Immun. 127, 2488–2495 (1981).
Ruberti, G. et al. J. exp. Med. 175, 157–162 (1992).
von Boehmer, H. A. Rev. Immun. 6, 309–326 (1988).
Leo, O., Foo, M., Sachs, D. H., Samelson, L. E. & Bluestone, J. A. Proc. natn. Acad. Sci. U.S.A. 84, 1374–1378 (1987).
Matsui, K. et al. Science 254, 1788–1791 (1991).
Demotz, S., Gray, H. & Sette, A. Science 249, 1028–1030 (1990).
Harding, C. V. & Unanue, E. R. Nature 346, 574–576 (1990).
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Weber, S., Traunecker, A., Oliveri, F. et al. Specific low-affinity recognition of major histocompatibility complex plus peptide by soluble T-cell receptor. Nature 356, 793–796 (1992). https://doi.org/10.1038/356793a0
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DOI: https://doi.org/10.1038/356793a0
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