Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Letter
  • Published:

Survival of Mouse Embryos after Freezing and Thawing

Abstract

COMPARED with the successful storage of spermatozoa and other tissue cells at sub-zero temperatures, attempts to preserve frozen mammalian eggs have been disappointing (reviewed in refs. 1 and 2), although a few pregnancies have resulted from the transfer of previously frozen fertilized rabbit eggs3 and unfertilized mouse eggs4 to recipient foster mothers. I now wish to describe a technique for obtaining a high proportion of viable embryos after subjecting them to sub-zero temperatures.

This is a preview of subscription content, access via your institution

Access options

Buy this article

Prices may be subject to local taxes which are calculated during checkout

Similar content being viewed by others

References

  1. Austin, C. R., The Mammalian Egg, 116 (Thomas, Springfield, Illinois, 1961).

  2. Hafez, E. S. E., Acta Endocrinol., 62, Suppl. 140, 24 (1969).

    Google Scholar 

  3. Ferdows, M., Moore, C. L., and Dracy, A. E., J. Dairy Sci., 41, 739 Abst. (1958).

    Google Scholar 

  4. Sherman, J. K., and Lin, T. P., Fertil. Steril., 10, 384 (1959).

    Article  CAS  Google Scholar 

  5. Whittingham, D. G., Nature, 220, 592 (1968).

    Article  ADS  CAS  Google Scholar 

  6. Dulbecco, R., and Vogt, M., J. Exp. Med., 99, 167 (1954).

    Article  CAS  Google Scholar 

  7. Whittingham, D. G., and Wales, R. G., Austral. J. Biol. Sci., 22, 1065 (1969).

    Article  CAS  Google Scholar 

  8. Mazur, P., Leibo, S. P., Farrant, J., Chu, E. H. Y., Hanna, M. G., and Smith, L. H., in The Frozen Cell, CIBA Foundation Symp. (edit. by Wolstenhome, G. E. W., and O'Connor, M.), 69 (Churchill, London, 1970).

    Google Scholar 

  9. Whittingham, D. G., J. Reprod. Fertil. (in the press).

  10. Brinster, R. L., Exp. Cell Res., 32, 205 (1963).

    Article  CAS  Google Scholar 

  11. Tarkowski, A. K., Acta Theriologica, 2, 251 (1959).

    Article  Google Scholar 

  12. Biggers, J. D., Moore, B. D., and Whittingham, D. G., Nature, 206, 734 (1965).

    Article  ADS  CAS  Google Scholar 

  13. Chang, M. C., Science, 111, 544 (1950).

    Article  ADS  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

WHITTINGHAM, D. Survival of Mouse Embryos after Freezing and Thawing. Nature 233, 125–126 (1971). https://doi.org/10.1038/233125a0

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1038/233125a0

This article is cited by

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.

Search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing