Abstract
COMPARED with the successful storage of spermatozoa and other tissue cells at sub-zero temperatures, attempts to preserve frozen mammalian eggs have been disappointing (reviewed in refs. 1 and 2), although a few pregnancies have resulted from the transfer of previously frozen fertilized rabbit eggs3 and unfertilized mouse eggs4 to recipient foster mothers. I now wish to describe a technique for obtaining a high proportion of viable embryos after subjecting them to sub-zero temperatures.
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References
Austin, C. R., The Mammalian Egg, 116 (Thomas, Springfield, Illinois, 1961).
Hafez, E. S. E., Acta Endocrinol., 62, Suppl. 140, 24 (1969).
Ferdows, M., Moore, C. L., and Dracy, A. E., J. Dairy Sci., 41, 739 Abst. (1958).
Sherman, J. K., and Lin, T. P., Fertil. Steril., 10, 384 (1959).
Whittingham, D. G., Nature, 220, 592 (1968).
Dulbecco, R., and Vogt, M., J. Exp. Med., 99, 167 (1954).
Whittingham, D. G., and Wales, R. G., Austral. J. Biol. Sci., 22, 1065 (1969).
Mazur, P., Leibo, S. P., Farrant, J., Chu, E. H. Y., Hanna, M. G., and Smith, L. H., in The Frozen Cell, CIBA Foundation Symp. (edit. by Wolstenhome, G. E. W., and O'Connor, M.), 69 (Churchill, London, 1970).
Whittingham, D. G., J. Reprod. Fertil. (in the press).
Brinster, R. L., Exp. Cell Res., 32, 205 (1963).
Tarkowski, A. K., Acta Theriologica, 2, 251 (1959).
Biggers, J. D., Moore, B. D., and Whittingham, D. G., Nature, 206, 734 (1965).
Chang, M. C., Science, 111, 544 (1950).
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WHITTINGHAM, D. Survival of Mouse Embryos after Freezing and Thawing. Nature 233, 125–126 (1971). https://doi.org/10.1038/233125a0
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DOI: https://doi.org/10.1038/233125a0
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