Abstract
Here we describe the identification of a novel vertebrate-specific centrosome/spindle pole-associated protein (CSPP) involved in cell-cycle regulation. The protein is predicted to have a tripartite domain structure, where the N- and C-terminal domains are linked through a coiled-coil mid-domain. Experimental analysis of the identified domains revealed that spindle association is dependent on the N-terminal and the coiled-coil mid domain. The expression of CSPP at the mRNA level was detected in all tested cell lines and in testis tissue. Ectopic expression of CSPP in HEK293T cells blocked cell-cycle progression in early G1 phase and in mitosis in a dose-dependent manner. Interestingly, mitosis-arrested cells contained aberrant spindles and showed impairment of chromosome congression. Inhibition of CSPP gene expression by small interfering RNAs induced cell-cycle arrest/delay in S phase. This phenotype was characterized by elevated levels of cyclin A, decreased levels of cyclin E and hyperphosphorylation of the S-phase checkpoint kinase Chk1. The activation of Chk1 may indicate a replication stress response due to an inappropriate G1/S-phase transition. Taken together, we demonstrate that CSPP is associated with centrosomes and microtubules and may play a role in the regulation of G1/S-phase progression and spindle assembly.
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References
Abraham RT . (2001). Genes Dev., 15, 2177–2196.
Adams PD, Sellers WR, Sharma SK, Wu AD, Nalin CM and Kaelin Jr WG . (1996). Mol. Cell. Biol., 16, 6623–6633.
Ali SH and DeCaprio JA . (2001). Semin. Cancer Biol., 11, 15–23.
Andersen JS, Wilkinson CJ, Mayor T, Mortensen P, Nigg EA and Mann M . (2003). Nature, 426, 570–574.
Augustin A, Spenlehauer C, Dumond H, Menissier-De Murcia J, Piel M, Schmit AC, Apiou F, Vonesch JL, Kock M, Bornens M and De Murcia G . (2003). J. Cell Sci., 116, 1551–1562.
Balczon R, Simerly C, Takahashi D and Schatten G . (2002). Cell Motil. Cytoskel., 52, 183–192.
Blom N, Gammeltoft S and Brunak S . (1999). J. Mol. Biol., 294, 1351–1362.
Bohmer RM and Ellwart J . (1981). Cytometry, 2, 31–34.
Burton JL and Solomon MJ . (2001). Genes Dev., 15, 2381–2395.
Butel JS . (2000). Carcinogenesis, 21, 405–426.
Butel JS and Lednicky JA . (1999). J. Natl. Cancer Inst., 91, 119–134.
Chen J, Saha P, Kornbluth S, Dynlacht BD and Dutta A . (1996). Mol. Cell. Biol., 16, 4673–4682.
Chomczynski P and Sacchi N . (1987). Anal. Biochem., 162, 156–159.
Clay-Farrace L, Pelizon C, Santamaria D, Pines J and Laskey RA . (2003). EMBO J., 22, 704–712.
Delattre M and Gonczy P . (2004). J. Cell Sci., 117, 1619–1630.
Doxsey S . (2001a). Nat. Rev. Mol. Cell Biol., 2, 688–698.
Doxsey SJ . (2001b). Nat. Cell Biol., 3, E105–E108.
Evan GI, Lewis GK, Ramsay G and Bishop JM . (1985). Mol. Cell. Biol., 5, 3610–3616.
Feijoo C, Hall-Jackson C, Wu R, Jenkins D, Leitch J, Gilbert DM and Smythe C . (2001). J. Cell Biol., 154, 913–923.
Fisk HA, Mattison CP and Winey M . (2002). Curr. Opin. Cell Biol., 14, 700–705.
Funderud S, Erikstein B, Asheim HC, Nustad K, Stokke T, Blomhoff HK, Holte H and Smeland EB . (1990). Eur. J. Immunol., 20, 201–206.
Gergely F . (2002). BioEssays, 24, 915–925.
Gergely F, Karlsson C, Still I, Cowell J, Kilmartin J and Raff JW . (2000). Proc. Natl. Acad. Sci. USA, 97, 14352–14357.
Gromley A, Jurczyk A, Sillibourne J, Halilovic E, Mogensen M, Groisman I, Blomberg M and Doxsey S . (2003). J. Cell Biol., 161, 535–545.
Hinchcliffe EH, Miller FJ, Cham M, Khodjakov A and Sluder G . (2001). Science, 291, 1547–1550.
Hinchcliffe EH and Sluder G . (2002). Oncogene, 21, 6154–6160.
Kaneko YS, Watanabe N, Morisaki H, Akita H, Fujimoto A, Tominaga K, Terasawa M, Tachibana A, Ikeda K, Nakanishi M and Kaneko Y . (1999). Oncogene, 18, 3673–3681.
Karsenti E and Vernos I . (2001). Science, 294, 543–547.
Khodjakov A, Cole RW, Oakley BR and Rieder CL . (2000). Curr. Biol., 10, 59–67.
Khodjakov A, Copenagle L, Gordon MB, Compton DA and Kapoor TM . (2003). J. Cell Biol., 160, 671–683.
Khodjakov A and Rieder CL . (2001). J. Cell Biol., 153, 237–242.
Kirschner M and Mitchison T . (1986). Cell, 45, 329–342.
Lange BM . (2002). Curr. Opin. Cell Biol., 14, 35–43.
Larsen JK, Munch-Petersen B, Christiansen J and Jorgensen K . (1986). Cytometry, 7, 54–63.
Leirdal M and Sioud M . (2002). Biochem. Biophys. Res. Commun., 295, 744–748.
Lengauer C, Kinzler KW and Vogelstein B . (1998). Nature, 396, 643–649.
Malumbres M and Barbacid M . (2001). Nat. Rev. Cancer, 1, 222–231.
Musacchio A and Hardwick KG . (2002). Nat. Rev. Mol. Cell Biol., 3, 731–741.
Nedelec F, Surrey T and Karsenti E . (2003). Curr. Opin. Cell Biol., 15, 118–124.
Nishitani H and Lygerou Z . (2002). Genes Cells, 7, 523–534.
Odeberg J, Wood T, Blucher A, Rafter J, Norstedt G and Lundeberg J . (2000). Biomol. Eng., 17, 1–9.
Okuda M, Horn HF, Tarapore P, Tokuyama Y, Smulian AG, Chan PK, Knudsen ES, Hofmann IA, Snyder JD, Bove KE and Fukasawa K . (2000). Cell, 103, 127–140.
Patzke S, Lindeskog M, Munthe E and Aasheim HC . (2002). Virology, 303, 164–173.
Pfleger CM and Kirschner MW . (2000). Genes Dev., 14, 655–665.
Prasanth SG, Prashanth KV, Siddiqui K, Spector DL and Stillman B . (2004). EMBO J., 23, 2651–2663.
Prasanth SG, Prasanth KV and Stillman B . (2002). Science, 297, 1026–1031.
Puntervoll P, Linding R, Gemund C, Chabanis-Davidson S, Mattingsdal M, Cameron S, Martin DM, Ausiello G, Brannetti B, Costantini A, Ferre F, Maselli V, Via A, Cesareni G, Diella F, Superti-Furga G, Wyrwicz L, Ramu C, McGuigan C, Gudavalli R, Letunic I, Bork P, Rychlewski L, Kuster B, Helmer-Citterich M, Hunter WN, Aasland R and Gibson TJ . (2003). Nucleic Acids Res., 31, 3625–3630.
Quintyne NJ and Schroer TA . (2002). J. Cell Biol., 159, 245–254.
Rappaport R . (1986). Int. Rev. Cytol., 105, 245–281.
Rasmussen AM, Smeland EB, Erikstein BK, Caignault L and Funderud S . (1992). J. Immunol. Methods, 146, 195–202.
Rieder CL, Faruki S and Khodjakov A . (2001). Trends Cell Biol., 11, 413–419.
Rieder CL and Palazzo RE . (1992). J. Cell Sci., 102, 387–392.
Rosenwald A, Wright G, Chan WC, Connors JM, Campo E, Fisher RI, Gascoyne RD, Muller-Hermelink HK, Smeland EB, Giltnane JM, Hurt EM, Zhao H, Averett L, Yang L, Wilson WH, Jaffe ES, Simon R, Klausner RD, Powell J, Duffey PL, Longo DL, Greiner TC, Weisenburger DD, Sanger WG, Dave BJ, Lynch JC, Vose J, Armitage JO, Montserrat E, Lopez-Guillermo A, Grogan TM, Miller TP, LeBlanc M, Ott G, Kvaloy S, Delabie J, Holte H, Krajci P, Stokke T and Staudt LM . (2002). N. Engl. J. Med., 346, 1937–1947.
Schiff PB, Fant J and Horwitz SB . (1979). Nature, 277, 665–667.
Stamenkovic I and Seed B . (1988). J. Exp. Med., 168, 1205–1210.
Sullivan CS and Pipas JM . (2002). Microbiol. Mol. Biol. Rev., 66, 179–202.
Wittmann T, Hyman A and Desai A . (2001). Nat. Cell Biol., 3, E28–E34.
Wolf E, Kim PS and Berger B . (1997). Prot. Sci., 6, 1179–1189.
Zhao H and Piwnica-Worms H . (2001). Mol. Cell. Biol., 21, 4129–4139.
Acknowledgements
We are thankful to K Solberg-Landsverk and M Strand for excellent assistance with the flow cytometry, Dr H Steen for help on the AX-70 microscope, and Drs S Funderud and E Boye for critical reading of the manuscript. The Norwegian Cancer Society and The Norwegian Research Council supported this work.
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Patzke, S., Hauge, H., Sioud, M. et al. Identification of a novel centrosome/microtubule-associated coiled-coil protein involved in cell-cycle progression and spindle organization. Oncogene 24, 1159–1173 (2005). https://doi.org/10.1038/sj.onc.1208267
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DOI: https://doi.org/10.1038/sj.onc.1208267
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