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HACS1 encodes a novel SH3-SAM adaptor protein differentially expressed in normal and malignant hematopoietic cells

Oncogene volume 20, pages 5373–5377 (2001)Cite this article

Abstract

SH3 and SAM domains are protein interaction motifs that are predominantly seen in signaling molecules, adaptors, and scaffold proteins. We have identified a novel family of putative adaptor genes that includes HACS1. HACS1 encodes a 441 amino acid protein that is differentially expressed in hematopoietic cells and has restricted expression in human tissues. Its SH3 domain is most similar to the same motif in Crk and its SAM domain shares homology with a family of uncharacterized putative scaffold and adaptor proteins. HACS1 maps to human chromosome 21q11.2 in a region that is frequently disrupted by translocation events in hematopoietic malignancies. Polyclonal antibodies against HACS1 recognized a 49.5 kDa protein whose mRNA is expressed in human immune tissues, bone marrow, heart, lung, placenta and brain. Cell lines and primary cells from acute myeloid leukemias and multiple myeloma patients express HACS1. Immunostaining and cellular fractionation studies localized the HACS1 protein predominantly to the cytoplasm.

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Acknowledgements

We thank E Wei for her technical assistance. JO Claudio is a recipient of Career Development Fellowship from the Canadian Blood Services. SJ Benn is a fellow of the Natural Science and Engineering Research Council of Canada. AK Stewart is supported with funds from the National Cancer Institute of Canada, the Canadian Institutes of Health Research, The ABC group and The Myeloma Research Foundation.

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Authors and Affiliations

  1. Experimental Therapeutics, Toronto General Research Institute, Ontario, Toronto, Canada

    Jaime O Claudio, Yuan Xiao Zhu, Nathan Falcioni & A Keith Stewart

  2. The Arthur and Sonia Labatt Brain Tumour Research Centre, Hospital for Sick Children, Toronto, Ontario, M5G 1X8, Canada

    Sally J Benn, Anu Heidi Shukla & C Jane McGlade

  3. Canadian Blood Services, Toronto Center, Toronto, Ontario, M5G 2M1, Canada

    Jaime O Claudio

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  1. Jaime O Claudio
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  2. Yuan Xiao Zhu
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Correspondence to A Keith Stewart.

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Claudio, J., Zhu, Y., Benn, S. et al. HACS1 encodes a novel SH3-SAM adaptor protein differentially expressed in normal and malignant hematopoietic cells. Oncogene 20, 5373–5377 (2001). https://doi.org/10.1038/sj.onc.1204698

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