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The HPV16 E5 oncogene inhibits endocytic trafficking

Oncogene volume 19, pages 6023–6032 (2000)Cite this article

Abstract

The small hydrophobic E5 protein of Human Papillomavirus type 16 (HPV16) binds to the 16-kDa subunit of the V-H+-ATPase. This binding has been suggested to interfere with acidification of late endocytic structures. We here used video microscopy, ratio imaging and confocal microscopy of living C127 fibroblasts to study the effects of E5. Various endocytic markers including the pH-sensitive probe DM-NERF coupled to dextran, TransFluoSpheres and TRITC-concanavalin A, were applied. In E5-transfected cells, none of these markers colocalized with the membrane permeable probe LysoTracker Red, which accumulates in acidic, late endocytic structures, or with a green fluorescent version of the small GTPase Rab7 labeling late endocytic structures. Importantly, however, late endocytic structures accumulating LysoTracker were still present in the E5-transfected cells. It is therefore concluded that HPV16 E5 perturbs trafficking from early to late endocytic structures rather than acidification.

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Acknowledgements

We thank M Stanley for supplying W12 cells. This work was supported by grants from the Danish Cancer Society, the Danish Medical Research Council, and the Novo Nordisk Foundation, the biotechnology program, Civil ingeniør Bent Bøgh og hustru Inge Bøgh's fond, Dagmar Marshalls foundation and Lykfeldts foundation. P Thomsen was working in the van Deurs laboratory with the support of a Ph.D. grant from the Faculty of Health Sciences, University of Copenhagen.

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Authors and Affiliations

  1. Department of Medical Anatomy, Structural Cell Biology Unit, The Panum Institute, University of Copenhagen, Blegdamsvej 3, Copenhagen N, DK-2200, Denmark

    Peter Thomsen, Bo van Deurs & Lars Kayser

  2. The Protein Laboratory, Institute for Molecular Pathology, The Panum Institute, University of Copenhagen, Blegdamsvej 3, Copenhagen N, DK-2200, Denmark

    Bodil Norrild

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  1. Peter Thomsen
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Thomsen, P., van Deurs, B., Norrild, B. et al. The HPV16 E5 oncogene inhibits endocytic trafficking. Oncogene 19, 6023–6032 (2000). https://doi.org/10.1038/sj.onc.1204010

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