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Inhibitory effect on the establishment of hepatic metastasis by transduction of the tissue plasminogen activator gene to murine colon cancer

Cancer Gene Therapy volume 6, pages 380–384 (1999)Cite this article

Abstract

Hepatic metastasis is a major factor in limiting the prognosis of patients with colon carcinoma. Recent investigations indicate a correlation between plasminogen activator profiles and hepatic metastasis. We examined the effectiveness of tissue plasminogen activator (tPA) gene therapy using a hepatic metastasis model of murine colon carcinoma. Murine colon carcinoma Colon 26 cells transduced with an MFGtPA retroviral vector (Colon 26/tPA) or an MFGLacZ retroviral vector (Colon 26/LacZ) were injected into the liver via the superior mesenteric vein of BALB/c mice, whose survival rates were checked daily. The mean survival rate of mice with hepatic metastasis induced by Colon 26/LacZ was 23.1 days, whereas that of mice with Colon 26/tPA was >100 days. The in vitro proliferation of Colon 26/tPA was comparable with that of Colon 26/LacZ, and antitumor immunity to wild-type Colon 26 cells was not induced after an intrahepatic injection of Colon 26/tPA. We suggest that transduction of the tPA gene to murine colon cancer is useful against the establishment of hepatic metastasis.

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Authors and Affiliations

  1. Department of Surgery II, Nagoya, Japan

    Shuji Hayashi, Itsuo Yokoyama, Yasushi Namii & Hiroshi Takagi

  2. First Department of Internal Medicine, Nagoya University School of Medicine, Nagoya, Japan

    Nobuhiko Emi

  3. Department of Transplant Surgery, Nagoya Daini Red Cross Hospital, Nagoya, Japan

    Kazuharu Uchida

Authors
  1. Shuji Hayashi
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  2. Itsuo Yokoyama
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  3. Yasushi Namii
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  4. Nobuhiko Emi
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  5. Kazuharu Uchida
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  6. Hiroshi Takagi
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Hayashi, S., Yokoyama, I., Namii, Y. et al. Inhibitory effect on the establishment of hepatic metastasis by transduction of the tissue plasminogen activator gene to murine colon cancer. Cancer Gene Ther 6, 380–384 (1999). https://doi.org/10.1038/sj.cgt.7700081

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  • DOI: https://doi.org/10.1038/sj.cgt.7700081

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