Summary
The RCK gene is a target of the t(11;14)(q23;q32) chromosomal translocation observed in human B-cell lymphoma, and the overexpression of its protein (rck/p54) by the translocation was shown to cause malignant transformation. The rck/p54 protein belongs to the DEAD box protein/RNA helicase family, which has a variety of functions such as translation initiation, pre-mRNA splicing and ribosome assembly. The expression of rck p54 in colorectal adenocarcinoma cells was examined by immunohistochemistry and Western blot analysis. The rck/p54 protein was found to be overexpressed in tumour tissues resected from 13 (50%) out of 26 cases of colorectal adenocarcinomas and two out of two (100%) cases of colonic severe dysplastic adenomas. In view of activities of rck/p54 determined in other tissue types, we suggest that rck/p54 may contribute to the cell proliferation and carcinogenesis at the translational level in the development of colorectal tumours.
Similar content being viewed by others
Article PDF
Change history
16 November 2011
This paper was modified 12 months after initial publication to switch to Creative Commons licence terms, as noted at publication
References
Akao, Y, Seto, M, Takahashi, T, Kubonishi, I, Miyoshi, I, Nakazawa, S, Tsujimoto, Y, Croce, CM & Ueda, R (1991). Molecular cloning of the chromosomal breakpoint of a B-cell lymphoma with the t(11;14)(q23;q32) chromosomal translocation. Cancer Res 51: 1574–1576.
Akao, Y, Seto, M, Yamamoto, K, Iida, S, Nakazawa, S, Inazawa, J, Abe, T, Takahashi, T & Ueda, R (1992). The RCK gene associated with t(11;14) translocation is distinct from the MLL/ALL-1 gene with t(4;11) and t(11;19) translocations. Cancer Res 52: 6083–6087.
Akao, Y, Marukawa, O, Morikawa, H, Nakao, K, Kamei, M, Hachiya, T & Tsujimoto, Y (1995). The rck/p54 candidate proto-oncogene product is a 54-kilodalton D-E-A-D box protein differentially expressed in human and mouse tissues. Cancer Res 55: 3444–3449.
Chuang, R-Y, Weaver, PL, Liu, Z & Chang, T-H (1997). Requirement of the DEAD-box protein Ded1p for messenger RNA translation. Science 275: 1468–1471.
Finlay, CA, Hinds, PW, Tan, T-H, Eliyahu, D, Oren, M & Levine, AJ (1988). Activating mutations for transformation by p53 produce a gene product that forms an hsp 70-p53 complex with an altered half-life. Mol Cell Biol 8: 531–539.
Grandori, C, Mac, J, Siebelt, F, Ayer, DE & Eisenman, RN (1996). Myc-Max heterodimers activate a DEAD box gene and interact with multiple E box-related sites in vivo. EMBO J 15: 4344–4357.
Ladomery, M, Wade, W & Sommerville, J (1997). Xp54, the Xenopus homologue of human RNA helicase p54, is an integral component of stored mRNP particles in oocytes. Nucleic Acids Res 25: 965–973.
Lazaris-Karatzas, A, Montine, KS & Sonnenberg, N (1990). Malignant transformation by an eukaryotic initiation factor subunit that binds to mRNA 5′ cap. Nature 345: 544–547.
Markwell, MAK, Hass, SM, Tolbert, NE & Bieber, LL (1981). Protein determination in membrane and lipoprotein samples: manual and automated procedures. In Methods in Enzymology, Lowenstein, ML (ed), pp. 296–303 Academic Press: New York
Rabbitts, TH (1994). Chromosomal translocations in human cancer. Nature 372: 143–149.
Rosen, F, Edery, I, Meerovitch, K, Dever, TE, Merrick, WC & Sonenberg, N (1990). Bidirectional RNA helicase activity of eukaryotic translation initiation factors 4A and 4F. Mol Cell Biol 3: 1134–1144.
Author information
Authors and Affiliations
Rights and permissions
From twelve months after its original publication, this work is licensed under the Creative Commons Attribution-NonCommercial-Share Alike 3.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-sa/3.0/
About this article
Cite this article
Nakagawa, Y., Morikawa, H., Hirata, I. et al. Overexpression of rck/p54, a DEAD box protein, in human colorectal tumours. Br J Cancer 80, 914–917 (1999). https://doi.org/10.1038/sj.bjc.6690441
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1038/sj.bjc.6690441
Keywords
This article is cited by
-
RNA-binding proteins and exoribonucleases modulating miRNA in cancer: the enemy within
Experimental & Molecular Medicine (2024)
-
The DEAD-box protein family of RNA helicases: sentinels for a myriad of cellular functions with emerging roles in tumorigenesis
International Journal of Clinical Oncology (2021)
-
Nucleic acid sensing pattern recognition receptors in the development of colorectal cancer and colitis
Cellular and Molecular Life Sciences (2017)
-
Negative regulation of the p300-p53 interplay by DDX24
Oncogene (2016)
-
Downregulation of anti-oncomirs miR-143/145 cluster occurs before APC gene aberration in the development of colorectal tumors
Medical Molecular Morphology (2013)