Abstract
Epithelial to mesenchymal transition (EMT) and extracellular matrix degradation are critical for the initiation and progression of tumor invasion. We have recently identified Krüppel-like factor 8 (KLF8) as a critical inducer of EMT and invasion. KLF8 induces EMT primarily by repressing E-cadherin transcription. However, how KLF8 promotes invasion is unknown. Here, we report a novel KLF8-to- matrix metalloproteinase (MMP)9 signaling that promotes human breast cancer invasion. To identify the potential KLF8 regulation of MMPs in breast cancer, we established two inducible cell lines that allow either KLF8 overexpression in MCF-10A or knockdown in MDA-MB-231 cells. KLF8 overexpression induced a strong increase in MMP9 expression and activity as determined by quantitative real-time PCR and zymography. This induction was well correlated with the MMP inhibitor-sensitive Matrigel invasion. Conversely, KLF8 knockdown caused the opposite changes that could be partially prevented by MMP9 overexpression. Promoter–reporter assays and chromatin and oligonucleotide precipitations determined that KLF8 directly bound and activated the human MMP9 gene promoter. Three-dimensional (3D) glandular culture showed that KLF8 expression disrupted the normal acinus formation, which could be prevented by the MMP inhibitor, whereas KLF8 knockdown corrected the abnormal 3D architecture, which could be protected by MMP9 overexpression. KLF8 knockdown promoted MDA-MB-231 cell aggregation in suspension culture, which could be prevented by MMP9 overexpression. KLF8 knockdown inhibited the lung metastasis of MDA-MB-231 cells in nude mice. Immunohistochemical staining strongly correlated the co-expression of KLF8 and MMP9 with the patient tumor invasion, metastasis and poor survival. Taken together, this work identified the KLF8 activation of MMP9 as a novel and critical signaling mechanism underlying human breast cancer invasion and metastasis.
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Introduction
Breast cancer survival rate falls from 90% for localized to 20% for metastatic disease. Metastasis requires invasive cells to detach from the localized tumors by epithelial to mesenchymal transition (EMT), degrade the extracellular matrix using proteases including matrix metalloproteinases (MMPs), survive the circulation as circulating tumor cells (CTCs) and colonize at distant locations. Understanding of the molecular mechanisms behind each of the steps is essential for targeting metastatic cells at early stage to improve patient survival.
Krüppel-like factor 8 (KLF8) is a GT-box (CACCC) binding dual-transcription factor that has a critical role in the regulation of cell cycle progression (Zhao et al., 2003; Wei et al., 2006; Wang et al., 2008; Mehta et al., 2009; Urvalek et al., 2010), transformation (Wang and Zhao, 2007), EMT and invasion (Wang et al., 2007). KLF8 recruits the CtBP co-repressor (van Vliet et al., 2000) to repress or the p300 and p300/CBP associated factor (PCAF) co-activators to activate target gene promoters (van Vliet et al., 2000; Zhao et al., 2003; Zhang et al., 2005; Wei et al., 2006; Wang et al., 2007; Evans and Liu, 2010; Lloyd, 2010; Urvalek et al., 2010). KLF8 expression is promoted by Src and phosphatidylinositol 3 kinase signaling (Zhao et al., 2003; Ding et al., 2005; Wang et al., 2008) and its transcription is activated by Sp1 (Wang et al., 2008) and KLF1 (Eaton et al., 2008) or inhibited by KLF3 (Eaton et al., 2008). KLF8 is also regulated by posttranslational sumoylation (Wei et al., 2006) and localization (Mehta et al., 2009; Rodriguez and Martignetti, 2009). We previously identified KLF8 as a focal adhesion kinase (FAK) downstream effector (Zhao et al., 2003). FAK is a critical regulator of both EMT and MMPs during breast cancer invasion and metastasis (Zhao and Guan, 2009). Like FAK, KLF8 is highly overexpressed in invasive human cancers including breast cancer (Wang and Zhao, 2007) and promotes breast cancer cell invasion (Wang et al., 2007). However, the mechanisms by which KLF8 promotes human breast cancer invasion have not been investigated.
In this study, we report that KLF8 regulates MMP9 expression by transcriptional activation in human breast cancer cells to promote cell invasion and metastasis in mice. We also show a strong correlation of KLF8 and MMP9 co-expression with the patient tumor metastasis. Our results support a potentially important role for KLF8 in promoting human breast cancer metastasis.
Results
KLF8 upregulates MMP9 expression and activity to promote cell invasion
Degradation of extracellular matrix and vascular basement membrane is required for a cancer cell to invade. MMP9 and MMP2 are among the major proteinases that have such a role. Interestingly, during the invasion of fibroblasts and other cell types, the expression and activity of these MMPs are found to be upregulated by FAK (Schlaepfer and Mitra, 2004), the upstream regulator of KLF8.
We first tested whether KLF8 is sufficient to promote invasion using our tet-off NIH3T3/KLF8 cells (Zhao et al., 2003). We found that induction of KLF8 expression led to a greater than threefold increase in the cell invasiveness (Figure 1A, left panel, compare induced (I) with uninduced (U)). We then determined whether KLF8 regulates the MMPs by in-gel zymography. We found that induction of KLF8 expression resulted in an increase in both pro-MMP9 and active MMP9, and only active MMP2 in the cells (Figure 1A, right-top panel, compare I with U). This result suggests that KLF8 regulate MMP9 mainly at the transcriptional level whereas KLF8 activation of MMP2 may be through an indirect mechanism. We have demonstrated that KLF8 promotes the MCF-10A cell invasiveness (Wang et al., 2007). To test whether KLF8 also regulates the MMPs in these cells, we performed in-gel zymography using MCF-10A, the non-invasive (MCF-7) and the highly invasive (MDA-MB-231) human breast cancer cell lines (Figure 1A, right-bottom panel). In agreement with the literature (Yao et al., 2001; Tsai et al., 2003; Rahman et al., 2006), there was little or no expression of MMP9 or MMP2 in MCF-10A-Mock cells (lane 1) (Wang et al., 2007) and MCF-7 (lane 3), but a dramatic increase in the MCF-10A-KLF8 (Wang et al., 2007) (compare lanes 2 to 1) and MDA-MB-231 cells (compare lanes 4 to 3).
We then established a MCF-10A cell line that expresses inducible KLF8 (10A-iK8) for an in-depth study. The inducible expression was verified by western blotting (Figure 1B, left panel, top two rows, compare lanes I with U). To determine KLF8 regulation of the MMPs at the message levels, we performed reverse transcriptase–PCR and quantitative real-time PCR and found that KLF8 increased mRNA expression of MMP9 but not MMP2 (Figure 1B, compare lanes or columns U with I). This is consistent with the change in pro-forms of the MMPs shown in Figure 1A.
To confirm the role of KLF8 activation of MMPs for the cell invasion, we performed in situ zymography using both the 10A-iK8 cells and MG6001 (Figure 1C). We found that KLF8 expression caused the matrix degradation (compare I+DMSO with U+DMSO), which is MG6001-sensitive (compare I+GM6001 with I+DMSO) and consistent with the cell invasiveness (Figure 1D, compare columns 3 to 2 and 1).
Taken together, these results suggest that KLF8 activation of MMPs is critical for its promotion of cell invasion.
MMP9 is a direct transcriptional activation target of KLF8
To test whether KLF8 regulates MMP9 transcription, we performed prompter (MMP9p) reporter (Lamar et al., 2008) assays. We found that KLF8 activated MMP9p in both NIH3T3 and MCF-10A cells (Figure 2a, compare the black with white columns). However, the activation domain deficient mutant (mKLF8) (Urvalek et al., 2010) was no longer able to do so (Figure 2a, compare the gray with black and white columns). These results suggest that KLF8 regulates MMP9p by transcriptional activation.
To test whether KLF8 directly activates MMP9p, we first analyzed the 1.6 kb promoter region and found six GT-boxes (Figure 2b). To map the promoter region essential for KLF8-responsive GT-box(s), we generated MMP9p truncation mutants (Figure 2b). Although deletion of the −1.6 kb through −541-bp region only caused a slight decrease in MMP9p activity (compare columns d6, d5–6 or d4–6 with wild type), deletion to −359 bp position caused a ∼80% decrease (compare columns d3–6 with wild type). Further deletion did not have an effect (compare columns d2–6 or d1–6 with d3–6). This result suggests that the region between −541 bp and −359 bp is essential for the promoter activation by KLF8. Interestingly, this region contains a GT-box (GT-box 3).
We disrupted this GT-box to see if it mediates KLF8 activation of MMP9p in the 10A-iK8 cells. Unlike the wild-type promoter that responded well to KLF8 in a time- and thus KLF8 level-dependent manner (Figure 2c, black columns), the mutant promoter (mGT-box 3) completely lost the responsiveness to KLF8 (Figure 2c, white columns).
To test whether KLF8 interacts with the endogenous MMP9 promoter in the 10A-iK8 cells, we performed chromatin immunoprecipitation assays (Figure 2d, top panel). We found that the promoter fragment containing the GT-box 3 was specifically co-immunoprecipitated by anti-KLF8 but not the control immunoglobulin G from I (compare lanes 3 to 2 and 4) but not U cells (lane 3, compare U with I). These results suggest that KLF8 could bind directly to the MMP9 promoter at the GT-box 3 site.
To see whether the KLF8-MMP9p interaction requires the GT-box 3, we performed biotinylated oligonucleotide precipitation assays (Figure 2d, bottom panel). We showed that KLF8 strongly bound to the wild-type (compare lanes 2 to 3) but not the GT-box 3 mutant (mGT) MMP9p fragment (compare lanes 1 to 2 or 3).
Taken together, these results suggest that KLF8 activates MMP9p by directly binding to it at the GT-box 3.
Aberrant upregulation of MMP9 by KLF8 in human breast cancer cells is critical for the cell invasion
For an in-depth understanding of how KLF8 regulates breast cancer cell invasion and metastasis, we also established the 231-K8ikd cell line that expresses inducible KLF8 short hairpin RNA. Green fluorescent protein (GFP) labels all the cells. The induced knockdown of KLF8 was confirmed by red fluorescent protein (RFP) expression (Figure 3A, compare panels f with c), western blotting (Figure 3B, top panel, compare lanes I with U) and quantitative real-time PCR (Figure 3B, middle and bottom panels, compare lanes I with U).
Using this cell line, we first tested whether KLF8 is required for the MMP mRNA expression. We found that the KLF8 knockdown caused a threefold decrease in MMP9 but had no effect on MMP2 mRNA levels (Figure 3B, middle and bottom panels, compare lanes I with U). In-gel zymography showed that on KLF8 knockdown, both the pro- and active MMP9 were dramatically reduced, whereas only active MMP2 but not the pro-MMP2 was decreased (Figure 3C, top panel, compare lanes I with U). MMP9p reporter assay indicated that the MMP9p activity was reduced by 60% when KLF8 was silenced (Figure 3C, bottom panel, compare lanes I with U).
Consistent with our previous observations using transient KLF8 knockdown (Wang et al., 2007), there was >50% reduction in the cell invasiveness on the inducible KLF8 knockdown. Importantly, this was correlated with a decrease in MMP9 protein levels (Figure 3D, compare columns or lanes 2 to 1). To determine the requirement of MMP9 for cell invasion, we transiently silenced MMP9 and found that the cell invasiveness dropped by 40% (Figure 3D, compare lanes or columns 4 to 3). To test whether MMP9 can rescue the cell invasiveness during KLF8 knockdown, we overexpressed MMP9 in the cells and found that the cell invasiveness was recovered regardless of KLF8 knockdown (Figure 3D, compare columns or lanes 6 to 5). These results suggest that the KLF8 knockdown-mediated decrease in MMP9 mRNA levels was responsible for the decrease in MMP9 protein expression and activity, which in turn limits the cancer cell invasiveness.
In addition, we found that in three-dimensional (3D) spheroid culture the normal structure of acini (Debnath et al., 2003a; Lee et al., 2007) formed by the 10A-iK8 cells was disrupted on induction of KLF8 expression and treatment with GM6001 partially protected this disruption (Supplementary Figure S2A). Conversely, the aberrant 3D structures (Debnath et al., 2003b; Carrio et al., 2005; McLachlan et al., 2006; Park et al., 2006) formed by the 231-K8ikd cells was partially reversed by either treatment with GM6001 or induction of KLF8 knockdown (as indicated by the expression of RFP in the cells) and the latter was prevented by MMP9 ectopic expression (Supplementary Figure S2B).
Taken together, these results suggest that KLF8 activation of MMP9 transcription is essential for maintaining MMP9 expression and activity in the breast cancer cells and the cell invasiveness possibly by disrupting the normal and maintaining the abnormal glandular structure.
KLF8-MMP9 axis is critical for the cell disaggregation in suspension
It is implicated that single-cell migration is important for breast cancer cell invasion, circulation and metastasis (Giampieri et al., 2009) and single CTC circulation is also critical for extravasation (Whipple et al., 2008; Balzer et al., 2009).
To test whether KLF8 has a role in maintaining the non-aggregated status of CTC-mimicking suspended breast cancer cells, we analyzed the aggregation ability of the 231-K8ikd cells (Figure 4). We found that the cell aggregation was induced within 30 min in suspension culture under I but not U conditions (Figures 4a and b, compare 5 to 2 or 6 to 4). This result was verified by an independent spheroid formation approach (Figures 4c and d, compare I with U). In this assay, the cells in which KLF8 was not knocked down (U, labeled by GFP only) grew sparsely. In contrast, when KLF8 was knocked (I, labeled by both GFP and RFP) the cells tended to aggregate together to form spheroids. To test whether MMP9 has a role in this regulation by KLF8, we performed rescue experiments and found that MMP9 overexpression prevented the spheroid formation regardless of KLF8 knockdown (Figures 4c and d, compare I+MMP9 and I).
These results suggest that the KLF8-MMP9 axis has an important role for single-cell invasion during breast cancer metastasis.
The aberrant overexpression of KLF8 is crucial for the lung metastasis
All the results described above pointed out a possibility that KLF8 expression is potentially important for the progression of tumor metastasis. To test this possibility, the 231-K8ikd cells were injected into nude mice through tail veins and the lung metastasis of the cells was examined (Figure 5). Knockdown of KLF8 resulted in approximately 75% reduction in the metastasis (Figure 5, compare I with U).
This result clearly demonstrates the critical role of KLF8 in the regulation of the breast cancer metastasis.
Aberrant co-overexpression of KLF8 and MMP9 is correlated with patient tumor metastasis
The above results support a possibility that KLF8 could promote MMP9 expression in human breast tumors and thus tumor invasion and metastasis. To test this, we examined the co-expression of KLF8 and MMP9 in human breast tumor tissues by immunohistochemistry using two tissue arrays from different sources. One of the arrays consists of 15 duplicates of breast tumor specimens with matched normal tissue counterparts (IMH-371, Figures 6A and B and Supplementary Table B). The other is composed of 75 specimens representing normal through aggressive breast cancer tumors (BR1503, Figure 6C and Supplementary Table C). As expected, KLF8 was predominantly localized in the nuclei whereas the MMP9 showed both cytoplasmic and extracellular presence (Figure 6A and Supplementary Figure S3). Among all specimens of the small group examined, 47% were positive for both KLF8 and MMP9 (Supplementary Table B). Importantly, the KLF8+/MMP9+ tumors were associated with a significantly higher metastasis rate (Figure 6B) and poor patient survival (Supplementary Table B). Similarly, among all the large set of 75 samples of normal tissue, benign tumor and malignant cyctosarcoma phyllodes, intraductal and invasive ductal carcinoma (Supplementary Table C), there was a statistically significant association of expression of KLF8 with MMP9 expression (P<0.001) (Figure 6C1). KLF8 and/or MMP9 expression was present almost exclusively in invasive ductal carcinoma in which 35 of 60 invasive ductal samples showed one or both compared with 1 of 15 samples of tissue other than invasive ductal carcinoma (in this one case it was MMP9 expression alone). Thus, there was a statistically significant association of KLF8, MMP9 or both expression with the invasive potential (P<0.001) (Figure 6C3). Indeed, in the 60 invasive ductal carcinoma samples only, there were 25 samples showing expression of both KLF8 and MMP9 and their expression was again statistically significantly associated with each other (P<0.001) (Figure 6C2).
Collectively, these results strongly suggest that KLF8 activation of MMP9 transcription and expression has a critical part in promoting human breast cancer invasion and metastasis.
Discussion
In this report, we identified the KLF8 regulation of MMP9 as a novel mechanism for human breast cancer cell invasion and metastasis. First, we showed that KLF8 promotes MMP9 transcription and activity in human breast cancer cells. Second, we demonstrated that the MMP activity is required for KLF8-induced cell invasion, the invasive 3D glandular architecture and the loss of cell–cell aggregation capability in suspension. Third, we provided evidence that KLF8 is required for breast cancer lung metastasis in model mice. Fourth, we identified a strong correlation of the co-expression of KLF8 and MMP9 with the patient tumor metastasis. This work has revealed a potentially significant role for KLF8 in promoting human breast cancer metastasis and shed a new light on the underlying molecular mechanisms.
This study identified MMP9 as a novel target of transcriptional activation by KLF8. This was demonstrated by (1) that KLF8 directly binds in vivo to the MMP9 promoter at the GT box and (2) both the KLF8-binding site and activation domain are required for its activation of the MMP9 promoter (see Figure 2). Notably, both this GT-box and the KLF8 activation domain are well conserved across species (see Supplementary Figure) (Urvalek et al., 2010), suggesting a broad significance of this important KLF8 regulation of MMP9. Our finding that KLF8 activates MMP9 transcription is also supported by our gene expression profiling studies using the 10A-iK8 cells (data not shown). It is known that MMP9 message stability is also aberrantly regulated in invasive breast cancer (Iyer et al., 2005; Yan and Boyd, 2007; Lamar et al., 2008). The message stability is controlled primarily by its 3′-UTR. Our MMP9 promoter reporter, however, (see Figure 1) does not contain the 3′-UTR-encoding template and there is no evidence that KLF8 might regulate 3′-UTR of any gene. Therefore, it is unlikely that KLF8 also has a role in stabilizing MMP9 message.
We have shown that KLF8 inhibits the E-cadherin transcription during EMT (Wang et al., 2007). We noticed that this EMT induction took several days although the E-cadherin mRNA began to decrease much earlier (data not shown). This observation suggests that the early repression of E-cadherin transcription by KLF8 is not sufficient to induce the EMT and destruction of the pre-existing E-cadherin protein is also essential. The shedding product of E-cadherin ectodomain, that is, the soluble E-cadherin, has been found in the blood and urine of metastatic cancer patients (Cristofanilli, 2006). Recent studies have demonstrated that the shedding is done by MMP9, MT1-MMP or MMP2 in lung or skin cancer cells during EMT (Covington et al., 2006; Dwivedi et al., 2006; Symowicz et al., 2007). On the other hand, treatment with the soluble E-cadherin has been shown to in turn upregulate the MMPs (Nawrocki-Raby et al., 2003) and promote the cell invasion (Symowicz et al., 2007). Additionally, overexpression of full-length E-cadherin can reduce MT1-MMP expression and MMP2 activity in the cells (Ara et al., 2000; Nawrocki-Raby et al., 2003). Our expression profiling data showed that MMP9 and MT1-MMP are upregulated within 24 h of KLF8 induction in the 10A-iK8 cells (data not shown). These results support an interesting possibility that during the early stage of KLF8-induced EMT, the pre-existing E-cadherin protein is downregulated by this shedding mechanism and the KLF8-activated MMPs may have a role for this potentially novel mechanism for the initiation of invasion at the early stage of breast cancer metastasis. Alternatively, KLF8 could use proteolytic degradation (Janda et al., 2006; Maeda et al., 2006; Yang et al., 2006) to destroy the pre-existing E-cadherin proteins. Nevertheless, the potentially mutual regulation between E-cadherin and the MMPs may represent a novel way by which KLF8 promotes the initiation of human breast cancer invasion by regulating both target gene transcription and protein stability. Using our inducible expression cell lines, we are vigorously testing this interesting possibility.
Single CTC circulation and extravasation is critical for breast cancer metastasis (Giampieri et al., 2009). The microtubule-based microtentacles formed on the surface of single CTCs are essential for the cells to adhere to and penetrate through the blood vessel endothelial layer during breast cancer cell extravasation (Whipple et al., 2008). Importantly, microtentacle formation is closely associated with vimentin (Whipple et al., 2008), a hallmark of EMT. Consistently, the disaggregation of the 10A-iK cells was clearly induced and maintained in suspension by KLF8 (Figure 4). As KLF8 has been shown to strongly induce EMT (Wang et al., 2007) and the KLF8-induced disaggregation depends on MMP9 (Figure 4b), it is plausible to speculate that the KLF8-to-MMP9 signaling may have an important role in the formation and maintenance of single breast cancer CTCs and microtentacles to promote distant metastasis.
The tumor cell disaggregation could lead to either difficult cell movement resulting in reduced metastasis or increased efficiency of colonization and thus increased metastasis. Our results have clearly demonstrated that KLF8 is critical for the lung metastasis (Figure 5). Therefore, it is likely that KLF8-promoted single-cell circulation helps the cell metastasis by enhancing the metastatic colonization.
We have previously demonstrated that KLF8 downregulation of E-cadherin is critical for breast cancer cell invasion (Wang et al., 2007). Here, we show that KLF8-mediated upregulation of MMP9 is also critical. In addition, the aberrant elevation of KLF8 is highly correlated with both the loss of E-cadherin (Wang et al., 2007) and the overexpression of MMP9 (Figure 6). These results strongly suggest that the downregulation of E-cadherin and upregulation of the MMP(s) by KLF8 are two important signaling arms required for maximizing KLF8-promoted breast cancer metastasis. Experiments are in progress to test this important possibility.
In summary, we have identified MMP9 as a novel target for transcriptional activation by KLF8 and demonstrated that the KLF8 regulation of MMP9 is critical for human breast cancer cell invasion. We have also shown that KLF8 expression is important for human breast cancer cell metastasis. Our results suggest a potentially significant role for KLF8 in the metastatic progression of human breast cancer. To date, MMP inhibitors have been proven less effective than expected in clinical trials (Coussens et al., 2002). Unlike MMP9, which is expressed in normal human tissue, the expression of KLF8 is barely detectable in normal epithelial cells (Wang and Zhao, 2007; Wang et al., 2007). In addition to MMP9, KLF8 also targets E-cadherin. Therefore, KLF8 may represent a novel favorable intervention target against breast cancer metastasis.
Materials and methods
Cell culture and reagents
MCF-10A (Debnath et al., 2003a; Wang et al., 2007) and the tet-off NIH 3T3/KLF8 cells were previously described (Zhao et al., 2003). The lung-prone MDA-MB-231 variant 4175 expressing thymidine kinase-GFP-luciferase was a kind gift from Dr Joan Massagué (Kang et al., 2005; Minn et al., 2005). These cells were maintained in either Dulbecco's modified Eagle's medium (DMEM)/F-12 or DMEM with 10% fetal bovine serum. To construct pLVUT-tTR-KRAB-KLF8, we replaced GFP with HA-KLF8 in the vector (Szulc et al., 2006). To construct pLVCT-tTR-KRAB-shKLF8, we cloned the short hairpin RNA duplex (Supplementary Table) into pLVTHM downstream of H1 promoter and then transferred the H1-shKLF8 cassette into pLVCT-tTR-KRAB (Szulc et al., 2006) and replacing the GFP with mCherry (RFP). The human MMP9 expressing lentiviral vector and MMP9 promoter reporter were previously described (Iyer et al., 2005; Lamar et al., 2008). To generate the MCF-10A line that expresses inducible KLF8 (10A-iK8) and the MDA-MB-231 line that expresses inducible KLF8 short hairpin RNA (231-K8ikd), the pLVUT-tTR-KRAB-KLF8 or pLVCT-tTR-KRAB-shKLF8 lentiviruses were used to infect the MCF-10A or 4175 cells and positive cells were cloned. The cell lines were maintained under U (in the absence of doxycycline or Dox) or I (in the presence of Dox) conditions depending on the experimental requirement.
In-gel zymography
The MMP activities were assayed as described (Jorda et al., 2005). Briefly, 5 × 105 cells in a six-well plate were cultured in serum-free medium for 16–24 h, and the conditioned medium was separated on an sodium dodecyl sulfate–polyacrylamide gel electrophoresis gel containing 1 mg/ml gelatin. The gel was washed with buffer I (Tris–HCl [pH 7.5] and 2.5% Triton X-100), incubated overnight in buffer II (150 mM NaCl, 5 mM CaCl2, 50 mM Tris–HCl [pH 7.6]) at 37 °C and stained with Coomassie blue. The clear bands indicate where MMPs degraded gelatin.
In situ zymography
The 10A-iK8 cells were grown for 72 h under U or I conditions, 106 cells were washed with DMEM and replated on DQ collagen fluorescein conjugate (Molecular Probes of Invitrogen, Carlsbad, CA, USA) coated cover glass and cultured with serum-free media for 16 h. The fluorescence release resulting from MMP cleavage of the matrix was visualized by microscopy. The MMP inhibitor GM6001 was included in some cultures.
Quantitative real-time PCR and western blotting
These assays were done essentially as previously described (Wang et al., 2007). See primer information in Supplementary Table. Anti-MMP9 (1:1000) was from Cell Signaling Technology, Inc. (Danvers, MA, USA).
Promoter reporter assays, chromatin immunoprecipitation and biotinylated oligonucleotide precipitation
These assays were performed essentially as previously described (Wang et al., 2007). For reporter assays, cells were transfected with reporter constructs with transient or induced expression of KLF8. For chromatin immunoprecipitation assays, the 10A-iK8 cells were cultured under U or I conditions for 3 days and processed for the analyses. For biotinylated oligonucleotide precipitation assays, the 10A-iK8 cells were cultured with Dox for 3 days and cell lysates were processed for analysis. See oligouncleotide information in Supplementary Table.
3D culture
3D cell culture was performed as previously described (Debnath et al., 2003a). The 10A-iK8 cells were propagated in DMEM/F-12 with fetal bovine serum and other supplements. Single cells in medium containing 5% Matrigel were seeded at a density of 5 × 104 cells/cm2 on a Matrigel-coated well. The 231-K8ikd cells in DMEM plus 10% fetal bovine serum were seeded at 2 × 104 cells/cm2. GM6001 was included in some experiments. In other experiments, the cells were infected with lentiviruses for 2 days to overexpress MMP9 before transfer to 3D culture.
Cell aggregation analysis
Trypsin-singularized cells were incubated in suspension (1 × 106 cells/ml) for various periods of time, a 50 μl volume of cells were carefully transferred to a cover slide. For spheroid formation in hanging drops, 2 × 104 cells were incubated in a 50 μl drop of complete medium hanging on the inner side of the lid of 12-well plate for 12 h. After the incubation, the drop was pipetted five times with a 200-μl standard tip and spread on a cover slide. Images of more than five random fields from at least three independent cultures were taken by phase contrast or fluorescent microscopy. Single cells or spheroids that contain at least 30 cells were counted.
Immunohistochemistry
Human breast cancer tissue arrays (IMH-371 from IMGENEX and BR1503 from US Biomax, Rockville, MD, USA) sections were baked for 1 h at 62 °C for rehydration and microwaved in 0.01 M sodium citrate for 5 min for antigen retrieval. After incubated in 3% H2O2 for 6 min, the sections were serum blocked for 30 min, incubated overnight at 4 °C with first antibodies in phosphate-buffered saline and subsequently with biotin-labeled secondary antibodies for 30 min, followed by a peroxidase-labeled avidin–biotin complex (Vector Laboratories, Burlingame, CA, USA) for 30 min. The sections were developed in 3,3-diaminobenzidine tetrahydrochloride for 2 min and counterstained with hematoxylin for 4 min. The stained sections were dehydrated, treated with xylene and mounted for microscopy. Positive staining was displayed in brown color.
Matrigel invasion assays
The tet-off 3T3/KLF8, 10A-iK8 or 231-K8ikd cells grown under U or I conditions for 24–48 h were assayed as previously described (Wang et al., 2007). In some experiments, transient MMP9 overexpression, knockdown or inhibition with GM6001 was included. The invasion rates were normalized to the U cells.
Bioluminescence imaging analysis of lung metastasis
All animal work was done in accordance with a protocol approved by the Institutional Animal Care and Use Committee. Female Balb/c nude mice (Taconic) 4–6 weeks old were used for all xenografting studies. For lung metastasis formation, 2 × 106 viable cells were washed and harvested in 0.1 ml phosphate-buffered saline and subsequently injected into the lateral tail vein. In all, 5 or 6 weeks after injection mice were anaesthetized using isoflurance and injected intraperitoneally with 150 mg/kg of D-luciferin (15 mg/ml in phosphate-buffered saline). Imaging was completed between 2 and 5 min after injection with a Xenogen IVIS system coupled to Living Image acquisition and analysis software (Caliper Life Sciences, Hopkinton, MA, USA). For BLI plots, photon flux (photons/s/cm2 per steradian) was measured with a region of interest drawn around the bioluminescence signal encompassing the thorax of the mouse in a prone position. A background value was subtracted that was obtained from a luciferin-injected control mouse.
Statistical analysis
Summary data are presented as mean±s.d. with a minimum of three observations per group. Unpaired, paired or single sample Student's t-test with the Bonferroni correction for the multiple comparisons was applied as appropriate. The two by two table for human data were analyzed by Fisher's exact test. Significance was determined by the alpha level of 0.05.
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Acknowledgements
We appreciate Dr Joan Massagué for kindly providing the 4175 cells and Dr Chunhong Yan and Qingfen Li and Beihai Jiang of Zhao lab for helpful comments. This work was supported by grants from NCI (CA132977) and Susan G Komen for Cure (KG090444 and KG080616) to JZ and a grant from NCI (CA129637) to CMD. XW is a Komen postdoctoral fellow.
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Wang, X., Lu, H., Urvalek, A. et al. KLF8 promotes human breast cancer cell invasion and metastasis by transcriptional activation of MMP9. Oncogene 30, 1901–1911 (2011). https://doi.org/10.1038/onc.2010.563
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DOI: https://doi.org/10.1038/onc.2010.563
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