Abstract
Manganese superoxide dismutase (MnSOD/SOD2) is a mitochondria-resident enzyme that governs the types of reactive oxygen species egressing from the organelle to affect cellular signalling. Here we demonstrate that MnSOD upregulation in cancer cells establishes a steady flow of H2O2 originating from mitochondria that sustains AMP-activated kinase (AMPK) activation and the metabolic shift to glycolysis. Restricting MnSOD expression or inhibiting AMPK suppresses the metabolic switch and dampens the viability of transformed cells indicating that the MnSOD/AMPK axis is critical to support cancer cell bioenergetics. Recapitulating in vitro findings, clinical and epidemiologic analyses of MnSOD expression and AMPK activation indicated that the MnSOD/AMPK pathway is most active in advanced stage and aggressive breast cancer subtypes. Taken together, our results indicate that MnSOD serves as a biomarker of cancer progression and acts as critical regulator of tumour cell metabolism.
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Introduction
Advances in the understanding of the molecular basis of cancer have placed mitochondria1 at the centre of numerous abnormalities observed in tumour cell metabolism2,3,4, differentiation5, proliferation6 and survival7,8. Either because of its direct impact on the cellular metabolism or its role as a hub for signal transduction, deregulation of intrinsic mitochondrial processes combined with failure to halt cell cycle progression results in the genesis and progression of tumours9,10,11,12,13. Among the many abnormal features of cancerous cells, a form of metabolism reliant on aerobic glycolysis is remarkable4, as it enables cell survival in the near absence of oxygen and provides the necessary building blocks to support vigorous proliferation. Recently, a growing number of studies aimed at defining mechanisms of mitochondrial deregulation in cancer have indicated that a deeper understanding of tumor cell metabolism will likely have an impact on therapeutics by enabling the development of targeted treatments with fewer complications and increased efficacy in preventing recurrence post therapy7,14,15,16,17,18.
In parallel with glycolytic metabolism19,20,21,22, high MnSOD expression23,24,25 is a distinctive feature of tumours particularly notable at advanced stages26,27. In healthy mitochondria, MnSOD directly regulates the metabolism of superoxide radical anions generated as a by-product of the electron transport chain. In isolation, MnSOD converts the diffusion-restricted, mild-oxidant superoxide radical into the diffusible, strong oxidant hydrogen peroxide (H2O2) and thereby critically changes mitochondria-driven signalling in the cell. Thus, MnSOD does not always act as a first-line mitochondrial antioxidant defense. Recently, a study by our group demonstrated that in the absence of matched upregulation of mechanisms of H2O2 removal, MnSOD overexpression is actually detrimental to the integrity of mitochondria and the maintenance of its energetic functions28. This indicates that either directly or indirectly29,30 MnSOD regulates mitochondrial energetic and signalling functions. Using mitochondria-depleted cancer cells, it was established that the abrogation of mitochondria-dependent regulatory functions results in the appearance of highly invasive, aggressive, glycolytic cellular phenotypes31. Taken together, these observations indicate that progressive MnSOD upregulation, which results in mitochondrial dysfunction, could participate in the appearance of malignant cellular phenotypes characterized by glycolytic metabolism.
In this report, results are presented showing that mitochondrial MnSOD upregulation leads to the activation of AMP-activated kinase (AMPK), a cellular metabolic master switch32,33 that directly enhances glycolysis. We also establish that in cancer cells, H2O2 (mtH2O2) released from mitochondria consequentially to MnSOD upregulation is the signal that engages AMPK to produce and sustain the Warburg effect, thereby enabling cancer cell survival.
Results
MnSOD upregulated in cancer cells promotes glycolysis
In luminal breast cancer samples stratified by stage, MnSOD expression was present at significantly elevated levels in progressing tumour stages (Fig. 1a–d). The levels of MnSOD increased with histologic tumour grade being highest at histologic grade III and lowest in healthy and hyperplastic benign tissue (Fig. 1d). Elevated MnSOD levels were also observed in advanced prostate (Supplementary Fig. 1a) and colon (Supplementary Fig. 1b) cancer tissue as compared with healthy tissue samples. In breast cancer, MnSOD levels were noted to be highest in triple-negative and Her2 subtypes (Supplementary Fig. 2a), elevated in luminal cancers and lowest in healthy control tissue, indicating an association between high MnSOD expression and tumour aggressiveness. This association was further strengthened by the epidemiologic analysis of published data34 on 5-year breast cancer survival, which negatively correlated with levels of MnSOD expression. Supplementary Fig. 2b shows the Kaplan–Meier distribution of 5-year breast cancer survival indicating a clear inverse relationship between survival and MnSOD levels. In all cancer types analysed (breast, prostate and colon), MnSOD expression was also correlated with expression of lactate dehydrogenase (LDH, Fig. 1b; Supplementary Fig. 1), a surrogate of glycolytic metabolism35,36 that is associated with poor prognosis. Quantification of total cell MnSOD and LDH fluorescence in breast (Fig. 1b), prostate and colon tissue (Supplementary Fig. 1c,d, respectively) indicated that these changes are significant and support a role for MnSOD upregulation in the switch to glycolysis, a common feature of aggressive cancer subtypes.
To assess whether MnSOD upregulation occurs during the progression towards malignancy, MCF10A (non-tumorigenic mammary epithelial cells) expressing oncogenic v-Src kinase under the control of an inducible oestrogen receptor promoter were used. Activation of v-Src expression by exposure of MCF10-Er-Src cells to tamoxifen resulted in MnSOD upregulation early during the process of malignant transformation (12 h, Supplementary Fig. 3) and progressively increased. Fully transformed cells (48–72 h)37,38 expressed the highest levels of MnSOD. The increase in MnSOD levels during transformation paralleled a strong metabolic shift towards glycolysis with notable reduction in the steady-state levels of ATP (Supplementary Fig. 3).
To begin to assess the impact of MnSOD upregulation on breast cancer cell metabolism, five isogenic cell lines stably overexpressing MnSOD were obtained. The control cell line expressing the empty vector (neo) and four additional clonal lines Mn1, Mn44, Mn11 and Mn28 expressing MnSOD at various levels (Fig. 2a) within the range observed in human breast cancer (Fig. 1d) were used to assess the impact of MnSOD expression on O2-dependent metabolism and glycolysis. In accordance with recent observations39, MnSOD activity increased with enhanced expression levels but not in a directly proportional manner (Fig. 2b). However, incremental increase in MnSOD dampened O2 uptake (Fig. 2c) in parallel to dissipating the mitochondrial electrochemical potential as assessed by JC-1 (Fig. 2d) and CMXROS (Fig. 2e). It was observed that when MnSOD levels were higher than five- to sixfold over basal (such as in the Mn44 clone) the electrochemical potential dropped in parallel with the reduction in the steady-state levels of ATP (Fig. 2f) and strong activation of compensatory glycolysis as assessed by lactate production (Fig. 2g). Elevated MnSOD expression levels were associated with progressive enhancement of H2O2 production (Fig. 2h), indicating a redirection in oxygen utilization from ATP production to H2O2 synthesis. Collectively, the findings summarized in Fig. 2 support the hypothesis that enhanced MnSOD expression sustains the metabolic switch in cancer cells. Further experiments using the H2O2 biosensor, roGFP targeted to mitochondria, indicated that H2O2 is locally generated in the organelle and that the increase in H2O2, as expected, paralleled the loss of mitochondrial electrochemical potential (Fig. 2h).
Using extracellular flow analysis (Seahorse Biosciences), we further examined the effect of MnSOD expression on mitochondrial respiration and the glycolytic rate. In agreement with the data shown in Fig. 2, cells expressing MnSOD at basal levels (neo) had significantly higher antimycin-inhibitable oxygen consumption rates (OCR) in comparison with Mn44 (moderate MnSOD expression) and Mn11 (high MnSOD expression Fig. 3a). Reserve respiratory capacity (the difference between maximal and basal respiratory rates) in all three cell lines was found to be significantly compromised indicating that in all cases mitochondria were functioning at the limit of their respiratory capacity even at baseline MnSOD activity in neo cells. Further confirmation of the increasing dependence of cells expressing high MnSOD levels on glycolysis was obtained in experiments where the extracellular acidification rate (ECAR) was measured (Fig. 3b). ECAR is a direct measure of lactic acid production by cells and thus of glycolytic activity. As shown in Fig. 3c, the OCR/ECAR values for neo cells indicate that these cells are predominantly utilizing mitochondrial metabolism for energy. Higher levels of MnSOD expression in Mn44 and Mn11 promoted an increase in glycolysis (Fig. 3d). The relevance of this finding was highlighted by the observation that in the metastatic and heavily glycolytic breast cancer cell line (MDA-MB-231), MnSOD expression levels were higher than those found in neo (MCF7 background; Fig. 4a). In comparison with MCF7/neo, glycolytic and metastatic cells (MDA-MB231/U2OS) had MnSOD expression that matched those obtained in the Mn11 clone. Silencing MnSOD in MDA-MB231 cells (Fig. 4b) reduced the glycolytic rate of these cells with a parallel decrease in ATP steady-state levels (Fig. 4e,f) indicating that MnSOD-driven glycolysis significantly contributes to the maintenance of ATP production in phenotypically aggressive breast cancer cells.
MnSOD upregulation activates AMP-activated kinase
AMPK is a key energetic sensor and regulator of cellular metabolism that is activated by either a reduction in the intracellular ratio ATP/AMP or by the elevation of intracellular H2O2 levels40. Because both a reduction in ATP and elevation of H2O2 were found to occur in parallel with MnSOD overexpression, we examined whether AMPK was activated in cells expressing incremental levels of MnSOD. Figure 5a shows that increasing levels of MnSOD expression increased AMPK activity as assessed both by the phosphorylation of active site Thr172 and the phosphorylation of the downstream target Acetyl CoA Carboxylase (ACC). Challenging the cells with exogenous H2O2, the product of MnSOD activity, significantly increased the phosphorylation and activity of AMPK in neo and Mn1 cells (Fig. 5a). Conversely, H2O2 only minimally increased AMPK phosphorylation and activity in Mn44 cells and did not further augment the phosphorylation or activity of AMPK in cells expressing high MnSOD levels (Mn11 and Mn28, Fig. 5a). These results indicated that the increase in MnSOD expression beyond levels found in Mn44 cells maximally activated AMPK by endogenously generating sufficiently high steady fluxes of H2O2 (Fig. 5a). We also confirmed that mtH2O2 was the molecular agent generated by MnSOD that led to AMPK activation. Neo cells were exposed to (0–20 μM) H2O2, and AMPK phosphorylation, the phosphorylation of its downstream target ACC and the intracellular steady-state levels of ATP were assessed in side-by-side experiments (Supplementary Fig. 4). Low H2O2 levels insufficient to cause changes in ATP fully activated AMPK, indicating that H2O2 can induce AMPK activation independently of variations in ATP levels. To further demonstrate that when MnSOD activity is elevated mtH2O2 mediates the activation of AMPK, we expressed mitochondria-targeted catalase (mt-catalase) that efficiently dismutates H2O2 into O2 and H2O in mitochondria. Restricting mtH2O2 outflow resulted in a marked decrease in AMPK activation, especially in cells expressing higher levels of MnSOD (Fig. 5b). In accordance with our prediction that high MnSOD expression levels lead to a predominantly glycolytic metabolism (in order to maintain ATP production), mt-catalase did not change steady-state concentrations of ATP in neo cells but significantly reduced ATP levels by an additional ~75% in Mn11 cells (Fig. 5c). Addition of mt-catalase also resulted in decreased glycolysis as assessed using lactate accumulation and the overall decrease in basal OCR/ECAR measured by extracellular flow analysis (Fig. 5d,e). Importantly, the increase in AMPK activity observed in the cells expressing high levels of MnSOD was also observed in breast, colon and prostate cancers (Fig. 5f,g). In addition, AMPK activation paralleled MnSOD upregulation during the course of MCF10-Er-Src transformation (Supplementary Fig. 3c), was enhanced in MDA-MB-231 in comparison with MCF7/neo (Fig. 4) and was suppressed following silencing of MnSOD in MDA-MB-231 (Fig. 4d). Together, these findings provide evidence for the involvement of MnSOD in promoting the glycolytic switch of aggressive breast cancer cells in an AMPK-dependent manner.
In view of recent reports implicating AMPK in the maintenance of NADPH levels in cancer cells, we also examined whether the MnSOD-directed activation of AMPK maintained steady-state NADPH levels in MnSOD-overexpressing cells (Supplementary Fig. 5). The levels of NADPH did not significantly change in cells overexpressing MnSOD when grown in complete media. However, in the absence of glucose, NADPH levels were diminished in MnSOD-overexpressing cells consistent with the high demand for NADPH in these cells that may have been sustained by the chronically elevated levels of H2O2 production. These results also indicate that MnSOD, in a glucose-dependent manner, contributes to activate NADPH synthesis possibly via AMPK (Supplementary Fig. 5), thereby sustaining cancer cell biosynthetic capacity, viability and proliferation. AMPK phosphorylation and activity (as assessed by ACC phosphorylation) were found to parallel MnSOD expression in a tumour-stage-dependent manner (Supplementary Fig. 6). AMPK activity was also found to be significantly increased in early malignant breast tumours as compared with control tissue and to increase progressively with tumour stage (Supplementary Fig. 6). In addition, in aggressive breast cancer subtypes, the elevation of MnSOD expression was strongly correlated with an increase in AMPK activation (Supplementary Fig. 6). This observation taken together with findings shown in Fig. 5f,g (that AMPK activation also parallels MnSOD upregulation in prostate and colon cancer) indicates the potential relevance of this finding as a mechanism to sustain aggressive cancer cell metabolism in multiple types of cancer.
MnSOD/AMPK direct the metabolic shift towards glycolysis
The activating effect of MnSOD upregulation on AMPK and the subsequent shift from cellular oxidative respiration to glycolysis was confirmed by silencing of MnSOD expression in MCF7/neo, Mn44 and Mn11 using short interfering RNA. Suppression of MnSOD expression in Mn44 and Mn11 was controlled to match levels of MnSOD expression equivalent to those found in resting MCF7/neo cells (Fig. 6a). AMPK phosphorylation analysed using western blot was observed to be reduced in Mn44 and in Mn11 cells transfected with MnSOD siRNA. Silencing of MnSOD caused significant suppression of glycolysis in Mn44 and Mn11 (Fig. 6b), while not significantly affecting MCF7/neo. Directly interfering with AMPK using a pharmacologic inhibitor (Compound C) significantly inhibited glycolysis in MCF7/neo, Mn44 and Mn11 cells as expected based on the prominent role of AMPK in activating glycolysis, but only affected ATP levels in Mn44 (mildly, ~−30%) and Mn11 (heavily ~ −85%), Fig. 6d. Thus, while neo cells were capable of using different routes for ATP synthesis, cells expressing higher levels of MnSOD appeared to have increasing dependence on glycolysis. This finding was further examined in cells devoid of AMPK’s catalytic subunit AMPKα1. To investigate the effect of MnSOD/AMPK in the induction of the Warburg effect, MnSOD was transfected into MCF-7 cells constitutively expressing AMPKα1 siRNA versus scrRNA. Results shown in Fig. 6e,f show that MnSOD overexpression in AMPK-competent cells recapitulated the strong repression of O2 uptake observed in Mn11 cells in parallel with an increase in the ECAR (an index of increased glycolytic metabolism). Conversely, overexpression of MnSOD in cells devoid of AMPKα1 produced only marginal effects on cellular metabolism that remained predominantly aerobic.
The dependence of MnSOD-overexpressing cells on glucose was also exemplified by the finding that Mn44 and Mn11 could only maintain steady levels of NADPH in a glucose-rich medium (Supplementary Fig. 5) consistent with the expectation that higher intracellular H2O2 leads to accelerated NADPH turnover. These observations are in agreement with recent published work demonstrating that one of the many important functions of AMPK activation in cancer is to maintain NADPH recycling and availability41 (Supplementary Fig. 5).
MnSOD overexpression upregulates glycolytic enzymes
Additional insight into the importance of the MnSOD/AMPK pathway for the glycolytic shift of cancer cells was obtained by determining whether MnSOD levels affected the expression and activity of the key glycolytic enzymes. Hexokinases are enzymes that catalyse glucose phosphorylation for entry in the glycolytic and pentose phosphate pathways, and phosphofructokinases (PFK) catalyse the rate-limiting step in the glycolytic pathway. Hexokinase and PFK activities were found to be enhanced in cells expressing high MnSOD levels in comparison with neo controls. PFK activity was increased consistent with a greater reliance of MnSOD-overexpressing cells on glycolysis (Supplementary Fig. 7) and the direct activating effect of AMPK on PFK2. In fact, it was confirmed that MnSOD overexpression in AMPK-competent cells resulted in remarkable phosphorylation of PFK2 on Ser466, an activating AMPK-dependent phosphorylation site42 (Supplementary Fig. 7b). MnSOD overexpression in AMPK-deficient cells did not result in increased PFK2 phosphorylation supporting our hypothesis that MnSOD sustains the glycolytic activity of cells where it is overexpressed by engaging AMPK-dependent signalling (Supplementary Fig. 7). Notably, a marked reduction in the expression levels of mitochondrial electron transfer chain complexes II, III, IV and V (Supplementary Fig. 8a) was observed in cells overexpressing MnSOD consistent with the observations of reduced mitochondrial potential and O2 uptake. In parallel, we analysed the expression of the key antioxidant enzymes in order to gain additional understanding of the mechanisms underlying the persistent elevation in H2O2 due to MnSOD upregulation. Catalase, glutathione reductase, thioredoxin and thioredoxin reductase were examined and only thioredoxin was significantly reduced in Mn44, Mn11 and Mn28 cells (Supplementary Fig. 8b). These results indicate that alterations in the expression and functioning of electron transfer chain complexes in MnSOD-overexpressing cells enhance mtH2O2 production unopposed by the major H2O2-scavenging systems.
CaMKII, a redox-sensitive kinase that activates AMPK
Data presented above indicated that the constitutive elevation of cellular H2O2 maintained AMPK activation thereby promoting the irreversible conversion of the cellular metabolism to glycolytic. The mechanism of MnSOD-dependent AMPK constitutive activation was next investigated with a focus on CaMKII, a kinase responsive to H2O2-driven oxidative activation upstream of AMPK43,44. Oxidation of CaMKII at residues M281 and M282 had previously been shown to result in chronic activation, and this constitutive activation of CaMKII was apparent even in cells overexpressing low levels of MnSOD (two- to threefold over basal, Mn1), Fig. 7a. Consistent with the hypothesis that AMPK is critical for maintaining increased glycolysis and steady-state ATP levels in MnSOD overexpressing cells, the CaMKII inhibitor KN93 dampened glycolysis and reduced the steady-state ATP levels in Mn44 and Mn11 cells (Fig. 7b). Importantly, inhibition of CaMKII resulted in robust dephosphorylation of AMPK at the active site Thr172 (Fig. 7c). Decreased AMPK phosphorylation was accompanied, as expected, by a reduction in AMPK activity as indicated by lower levels of phosphorylated ACC (Fig. 7c), a downstream target of AMPK. This observation indicates that CaMKII oxidation by mtH2O2 leads to its constitutive activation and consequentially the activation of AMPK. Inhibition of CaMKII markedly reduced the viability of cells expressing high MnSOD levels (Fig. 8). The potential clinical importance of this finding was furthered by comparing the effect of CaMKII inhibitor KN93 with two commonly used breast cancer chemotherapeutic compounds, paclitaxel (a mitotic inhibitor) and 5-fluorouracil (a thymidylate synthase inhibitor). Exposure to KN93 resulted in 60–70% cell death of Mn44 and Mn11 cells while only minimally reducing the viability of MCF7/neo cells by ~20%, which was in clear contrast to the effect of paclitaxel and 5-flurouracil, which were far more efficient in killing MCF7/neo (Fig. 8). This observation indicated that cells expressing high MnSOD levels are far more sensitive to CaMKII inhibition than to paclitaxel or 5-fluorouracil.
Glycolysis maintains cells overexpressing MnSOD viable
The dependence of cells expressing high MnSOD levels on glycolysis was further examined by conducting experiments where glycolysis was inhibited using 2-deoxy-D-glucose (2-DG). MCF7/neo, Mn44 and Mn11 cells were treated with 2-DG (a potent glucose analogue that cannot be metabolized) and then apoptosis was measured by flow cytometry (Fig. 9). 2-DG produced marked cell death among Mn11 cells compared with MCF7/neo controls. The inhibition of glycolysis dramatically lowered steady-state ATP levels in Mn44 and Mn11 cells, while only moderately inhibiting ATP production in neo cells (Fig. 9b). These results indicate that glycolysis significantly contributes to ATP production in MCF7/neo cells, but accounts for most, if not all, of the ATP production in cells expressing high MnSOD levels. Accordingly, we found that supplementation of media with substrates that support glycolytic metabolism (pyruvate and uridine) stimulated Mn11 and Mn28 proliferation without affecting MCF7/neo or Mn1 growth rates (Supplementary Fig. 9). The proliferation rate of Mn11 and Mn28 in the presence of pyruvate and uridine was comparable to the baseline proliferation rate of MCF7/neo, dismissing the concept that MnSOD per se is a tumour suppressor. Rather, MnSOD imposes a form of metabolism that requires glycolysis-supportive substrates to maintain rapid cellular proliferation.
Activation of the MnSOD/AMPK axis suppresses apoptosis
MnSOD is known to promote cell survival and inhibit apoptosis by mechanisms that remain poorly defined45. Since AMPK has been implicated in upregulating Bcl-2/survivin expression46, we examined the possibility that in addition to the metabolic effects described above, MnSOD/AMPK fostered cancer cell survival by upregulating anti-apoptotic factors such as survivin and Bcl-2. Cells expressing high MnSOD levels also had concurrent increases in survivin and Bcl-2 (Fig. 10a,b). To test the involvement of AMPK in the induction of survivin and Bcl-2 expression by MnSOD, AMPK expression was reduced using siRNA, which resulted in decreased Bcl-2 and survivin levels in both Mn44 and Mn11 cells. This observation implicates AMPK as a critical mediator of MnSOD-induced anti-apoptosis (Fig. 10c). Expression of MnSOD and survivin/Bcl-2 was also similarly increased in human cancer tissues compared with control tissue (Fig. 9d). Furthermore, KN93 was observed to be a potent inducer of apoptosis of MnSOD-overexpressing cells, likely because of additive effects in suppressing AMPK-driven metabolic and anti-apoptotic routes (Fig. 8). These findings support a critical role for MnSOD/oxCaMKII/AMPK in the maintenance of glycolytic cancer cell survival while adding mechanistic insight to the known anticancer effect of CaMKII inhibition45,47. MnSOD upregulation also repressed the cleavage of effector caspases 3 and 7 while activating caspase 9 and downregulating p53 expression (Supplementary Fig. 10). These findings further substantiate the critical role of MnSOD in adaptative responses that favour cancer cell survival. Our studies also indicate that MnSOD upregulation produced phenotypes that metabolically and functionally resemble more malignant cells such as those that occur in late-stage and aggressive subtypes of breast cancers. This conclusion was further supported by experiments using MCF10-Er-Src cells where MnSOD upregulation was shown to be essential for the survival of transformed cells (Supplementary Fig. 11). Silencing MnSOD before induction of MCF10A-Er-Src transformation induced by tamoxifen led to >70% cell death 48 h after tamoxifen stimulation. Although MnSOD ectopic expression did not significantly increase metastasis of xenografts produced in NOD/SCID mice, enhanced MnSOD expression in breast cancer cells conferred characteristics of aggressive cancer cells such as glycolytic metabolism (Figs 2, 3), drug resistance (Fig. 8) and enhanced capacity to grow in soft agar (Supplementary Fig. 12).
Discussion
Investigations dedicated to unravelling the role of MnSOD in tumorigenesis and cancer progression have yielded conflicting results in regard to the nature and potential effects of MnSOD in influencing cancer risk, prognosis, survival, susceptibility to therapy and likelihood of relapse. Recently, the consideration of MnSOD expression in specific contexts along with the functional status of other cellular antioxidant systems led to the more encompassing idea that MnSOD contributes to the progression of tumours towards aggressive phenotypes in which MnSOD enhances anti-apoptotic defenses and increases invasion47,48. The role of MnSOD as a primary participant and director of the malignant transformation process has been less appreciated; however, MnSOD and its product H2O2 have recently been shown to play critical roles in controlling OCRs and glucose uptake in mouse embryonic fibroblasts as these cells progress through the cell cycle from quiescence to proliferative states49 and in a variety of other systems where MnSOD and H2O2 affect survival and cellular function50,51,52,53,54,55,56. Our results provide direct evidence in support of the concept that MnSOD strongly suppresses mitochondrial oxidative metabolism while bolstering glycolysis by mechanisms that involve the rerouting of O2 towards the production of a steady flow of mtH2O2. MnSOD expression is regulated by inflammatory57,58, metabolic and stress-activated transcription factors59,60,61 and is generally expressed in response to an acute insult62,63. While the possibility that MnSOD provides protection against stress when acutely upregulated has been extensively scrutinized64,65,66, our results indicate that when persistently overexpressed at levels found in human cancers, MnSOD contributes to the progressive deterioration of mitochondrial bioenergetics and the activation of glycolysis via mtH2O2 production and AMPK activation. Interestingly, a similar role for SOD1, a cytosolic superoxide dismutase, has been described67. Although through a different mechanism (association with two casein kinase 1-gamma homologues), it was demonstrated that H2O2 generation was required to suppress mitochondrial respiration and stimulate glycolysis indicating, as proposed here, that the manipulation of intracellular levels of H2O2 may be of therapeutic utility in cancer. In fact, according to the results shown in Figs 1, 2, 3, 4, 5 and Supplementary Figs 1,2, the persistent upregulation of MnSOD with parallel increase in steady-state levels of H2O2 seem to be a distinctive feature of transformed cells that appears to satisfy critical energetic and metabolic necessities. Moreover, we found that repressing signals originating from MnSOD overexpression (such as mtH2O2, oxCaMKII and AMPK) strongly inhibit glycolysis without reactivating oxidative-based metabolism in mitochondria. Consequently, the interruption of MnSOD/mtH2O2/oxCaMKII/AMPK signalling results in the metabolic collapse of cells derived from advanced-stage cancers (MDA-MB-231 and transformed MCF10A-Er-Src) resulting in marked cell death. mtH2O2 was identified as the agent that integrates MnSOD-dependent mitochondrial signalling with the activation of AMPK, which was confirmed in experiments where we attenuated mtH2O2 levels by ectopically expressing mitochondria-targeted catalase, which rapidly dismutates H2O2 preventing its long-range effects. The effect of mitocatalase in repressing glycolysis in MnSOD-overexpressing cells indicates that there is an aggressive cancer-specific mechanism of glycolysis centred on the mitochondria-driven activation of the MnSOD/AMPK axis. This conclusion is based on the observation that silencing MnSOD or inhibiting AMPK reduces glycolysis to baseline levels while only marginally affecting ATP levels in cells expressing low levels of MnSOD (MCF7/neo cells). Inhibition of glycolysis with 2-DG reduced steady-state ATP levels in all cell lines examined but only killed those expressing elevated MnSOD levels such as Mn44 and Mn11 cells. Taken together, with the observation that mitochondria-driven ATP production was severely impaired in Mn44 and Mn11 lines, it is proposed that MnSOD promotes survival independently of oxygen (a clinically relevant problem) at the expense of metabolic flexibility. In this regard, the identification of a specific mitochondria-to-AMPK signalling axis that is activated by MnSOD upregulation and dependent on mtH2O2 in cancer cells can potentially be exploited for cancer therapeutic as this is not likely to interfere with normal glycolytic processes in healthy cells. This is especially important in view of recent findings demonstrating that AMPK activation is critical for the maintenance of cancer cell biosynthetic activity68.
On investigating the possible mechanisms by which AMPK is maintained constitutively activated in cells overexpressing MnSOD, we identified CaMKII as the redox-sensitive kinase activated upstream of AMPK, thereby translating the persistent increase in intracellular H2O2 into constitutive AMPK activation. The critical role of CaMKII in sustaining metabolic alterations required for cancer cell survival was illustrated by the effectiveness of KN93 in inhibiting AMPK phosphorylation and activity, and by its effectiveness in selectively killing MnSOD over-expressing cells as compared with neo control cells. Collectively, these experiments identified a mechanistic basis for the development of KN93 as an anticancer agent.
The likely clinical relevance of the data presented herein is further supported by the strong parallels between the results obtained in cell lines where MnSOD expression was manipulated and tissues obtained from patient biopsies where MnSOD, Bcl-2 and survivin were strongly upregulated in parallel with AMPK activation. The finding of elevated Bcl-2 and survivin expression in tumours has been generally associated with poor prognosis; therefore, mechanisms to suppress the expression of these anti-apoptotic proteins in tumours are generally regarded as attractive therapeutic goals. By specifically targeting the MnSOD/mtH2O2/AMPK axis, treatments may be developed to limit the expression of Bcl-2 and survivin, thereby diminishing the reliance on existing therapies that eliminate cancer cells but have prominent detrimental off-target effects.
MnSOD upregulation, AMPK activation and a gradual shift towards glycolysis as described in this work are to be regarded as part of a multifaceted process that occur in carcinogenesis. We see the MnSOD/oxCaMKII/AMPK-dependent metabolic switch in cellular metabolism as a necessary occurrence for the successful establishment of malignancy that, based on the results presented herein, permits cells to survive oxygen deprivation while strongly promoting anti-apoptotic defenses. As alterations in cellular metabolism from mitochondria-based to glycolytic encompasses relevant to almost all malignant solid tumours, we think that these data identify the MnSOD/H2O2/AMPK axis as a primary regulator of the Warburg effect and as such, a valuable new piece to the complex puzzle that is tumour progression.
Methods
Human patient sample analysis
De-identified human tissue samples were obtained from the University of Illinois at Chicago tissue bank according to IRB exemption note 20110082-58687-1/OPRS/UIC. Multiple images were acquired from at least six individual normal and six cancerous breast, prostate and colon tissues, as identified by Dr Andre Kajdacsy-Balla, a clinical pathologist. Representative images were used for Figs 1, 4f, 9 and Supplementary Fig. 1.
Fluorescent immunohistochemistry
Slides were blocked with regular fetal calf serum and incubated with primary antibody (MnSOD, LDH, diluted 1:1,000 in 1 × TBST, Abcam, Cambridge, MA, USA), overnight at 4 °C. After rinsing in TBST, sections were incubated with Alexafluor-conjugated secondary antibodies (Invitrogen/Life Sciences, Grand Island, NY, USA). Slides were examined on a Nikon ECLIPSE E400 microscope and documented using the SPOT Advanced version 4.0.1 software.
TMA analysis
MnSOD expression in multiple molecular subtypes of breast cancer was assessed using immunofluorescent imaging of tissue microarray (TMA)-1005 (Protein Biotechnologies, Ramona, CA, USA). Protein was blocked with normal serum and incubated with anti-MnSOD, anti-pAMPK-Thr172 (diluted 1:100 in 1 × TBST containing 0.1% bovine serum albumin, Abcam) overnight at 4 °C. The slides were then incubated with Alexafluor-conjugated secondary antibodies (Invitrogen/Life Sciences) and examined on an Apotome (Zeiss, Jena, Germany). Relative fluorescent intensity was measured using ImageJ, and RFU values were associated with molecular subtypes described previously69.
Quantitation of relative fluorescent units
Corrected total fluorescence was calculated as described previously70. Three background samples were taken per selection to assure proper calibration.
Measurement of MnSOD activity
MnSOD was immunoprecipitated from cell lysates using a mouse anti-MnSOD antibody conjugated to protein A-bound resin. Protein (20 μg) from lysates was loaded into PBS solutions containing 20 μg anti-MnSOD antibody. MnSOD activity was calculated compared with MCF7/neo based on the ability of MnSOD-bound to resin to inhibit the reduction of 50 μM cytochrome c Fe(III) reduced by hypoxanthine (50 μM)/xanthine oxidase (10 mU ml−1) system. Incubations of MnSOD immobilized to resin, cytochrome c and xanthine oxidase/hypoxanthine were vigorously agitated. Total cytochrome c reduction was measured by absorbance at 550 nm after 30 min incubation at room temperature.
Confocal microscopy
Cells were plated onto MatTek glass-bottomed culture dishes and allowed to adhere overnight. After treatments, cells were washed with PBS and fixed with 4% paraformaldehyde (PFA). Cells were permeabilized using cold methanol. Images were recorded using a Zeiss LSM510UV META confocal microscope.
Amplex red assay
H2O2 production from cells was measured using the Amplex Red Hydrogen Peroxide/Peroxidase Assay Kit (Invitrogen/Life Sciences).
Mitocatalase transfection
Cells were grown in a six-well plate in media. Mitocatalase adenovirus was added to treatment wells and allowed to infect cells for 24 h. Virus was a generous gift from Dr J. Andres Melendez (SUNY, Albany).
ATP assay
Cells were grown in a white-walled, clear bottom 96-well plate in media, transferred to glucose-free media with galactose and then analysed for ATP production using the Mitochondrial Tox-Glo Assay Kit (Promega, Madison, WI, USA) using a spectrophotometer.
JC-1 assay
Cells were grown in MatTek confocal dishes. On achieving 70% confluency, cells were incubated in 5 μM JC-1 for 20 min at 37 °C then washed 2 × in PBS and immediately imaged by confocal microscopy.
Glycolysis assay
Cells were grown in a 96-well plate in media, transferred to serum-free media for 24 h and then analysed for glycolytic activity using the Glycolysis Cell-Based Assay Kit (Cayman Chemical, Ann Arbor, MI, USA).
MnSOD/AMPK silencing
MnSOD, AMPK and scrambled siRNA were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA) and incorporated into cells via electroporation using Amaxa Nucleofector Technology (Lonza, Basel, Switzerland). After electroporation, cells were plated and incubated for 24 h, and then collected for protein analysis by western blot or plated for glycolysis measurements.
Flow cytometry
Cells were analysed using YOPRO and propidium iodide for apoptosis and viability by flow cytometry at the University of Illinois at Chicago Research Resources Flow Cytometry Service.
PFK, pyruvate kinase and hexokinase activity assays
Colorimetric assay kits were purchased from Abcam. Enzyme activities were measured following the manufacturer’s instructions. In brief, equal amounts (1–2 × 106 cells) of each cell line were lysed with cold assay buffer on ice, and the supernatant was mixed with freshly prepared reaction mix in 96-well plates. The plates were then incubated at 37 °C for 60 min, and absorbance was measured every 10 min. Two time points (20 and 60 min) were chosen to calculate the enzyme activity.
Transformation of MCF10A-Er-Src and silencing of MnSOD
For silencing of MnSOD in MCF10A(Er-Src) cells, Lipofectamine LTX (Invitrogen) was used as per the manufacturer’s instructions. Silencing was performed using control scrRNA and MnSOD siRNA (Santa Cruz Biotechnologies). Briefly, cells were incubated with either scrRNA or siRNA at 37 °C for 8 h, and then treated with 1 μM tamoxifen (Sigma Aldrich). The cells were harvested for analysis 48 h after tamoxifen was added. For transformation MCF10A-Er-Src cells were plated at 50% confluency and maintained in 1 μM tamoxifen for the duration of the experiment as indicated. MCF10AEr-Src cells were a kind gift from Dr Kevin Struhl (Harvard University).
Statistical analysis
Statistical analysis was performed with GraphPad InStat by using one-way analysis of variance with Student–Newman–Keuls comparison. A value of P<0.05 was considered significant, whereas values of P<0.01 and P<0.001 were considered highly significant.
Additional information
How to cite this article: Hart, P. C. et al. MnSOD upregulation sustains the Warburg effect via mitochondrial ROS and AMPK-dependent signalling in cancer. Nat. Commun. 6:6053 doi: 10.1038/ncomms7053 (2015).
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Acknowledgements
We are indebted to Drs Larry W. Oberley (in memoriam) and Frederick Domann (University of Iowa) for the generous gift of cell lines stably overexpressing MnSOD, Dr Kevin P. Claffey (University of Connecticut) for the generous gift of MCF-7-AMPKα1 −/− cells, Dr Kevin Struhl (Harvard University) for the gift of the MCF10A lines expressing v-Src under ER induction, Patricia Mavrogianis, Andrew Hall and Emily Ionetz at the Research Histology Core at the University of Illinois at Chicago for providing histology services, Dr Soumen Bera, Emmanuel Ansong, Paula Green, Alyssa Master and M. Saqib Baig for technical assistance, and for funding provided by the US Department of Defense (ARO no. 61758-LS) to M.G.B, the NCRR/NIH (S10RR027848) to M.G.B., 1R21CA182103-01 to A.M.D. and M.G.B. K.A.-F. and P.C.H. were supported by NIH T32 (HL072742). We also acknowledge gifts from RO1 CA101053 to A.M.D., NIH P01 HL60678 to R.D.M., CAPES—Coordenação de Aperfeiçoamento Pessoal, Ministério da Cie^ncia e Tecnologia/Brazil—A109/2013 to M.G.B. and M.E.L.C.
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The key experiments were performed by P.C.H., K.A.-F., M.M., A.L.A., D.N.E., D.G. P.C.H., K.A.-F., M.M., A.L.A., D.N.E., A.M.D., J.H.S. and M.G.B. conceived and designed experiments. Data interpretation and hypothesis were generated by P.C.H., K.A.-F., M.M., A.M.D., A.K.-B., R.D.M., M.E.L.C., J.H.S., M.G.B. D.N.E., A.K.-B. and A.M.D. provided exclusive reagents and model systems. A.K.B. built, classified and analysed data from T.M.A. A.M.D., R.D.M., J.H.S., M.E.L.C. and M.G.B. wrote the manuscript. M.G.B. had the general oversight of the project.
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Hart, P., Mao, M., de Abreu, A. et al. MnSOD upregulation sustains the Warburg effect via mitochondrial ROS and AMPK-dependent signalling in cancer. Nat Commun 6, 6053 (2015). https://doi.org/10.1038/ncomms7053
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DOI: https://doi.org/10.1038/ncomms7053
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