Abstract
Innate lymphoid cells (ILCs) communicate with other haematopoietic and non-haematopoietic cells to regulate immunity, inflammation and tissue homeostasis. How these ILC lineages develop and are maintained is not clear. Here we show that WASH is highly expressed in the nucleus of group 3 ILCs (ILC3s). WASH deletion impairs the cell pool of NKp46+ ILC3s. In NKp46+ ILC3s, WASH recruits Arid1a to the Ahr promoter thus activating AHR expression. WASH deletion in ILC3s decreases the number of NKp46+ ILC3s. Moreover, Arid1a deletion impedes AHR expression and impairs the maintenance of NKp46+ ILC3s. Therefore, WASH-mediated AHR expression has a critical function in the maintenance of NKp46+ ILC3s.
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Introduction
Innate lymphoid cells (ILCs) reside in mucosal surfaces to potentiate immune responses, sustain mucosal integrity and maintain tissue homeostasis. ILCs can be categorized into three groups based on their signature effector cytokines, analogous to the classification of T cell subsets1. Group 1 (ILC1) cells are characterized by their capacity to secrete interferon γ (IFN-γ) in response to interleukin 12 (IL-12), IL-15 and IL-18 (refs 2, 3). Group 2 (ILC2) cells generate type 2 T helper (Th2) cell cytokines such as IL-5, IL-9 and IL-13 in response to IL-25 and IL-33 stimulation4,5,6. Group 3 (ILC3) cells produce IL-17 and IL-22 upon stimulation with IL-1β and IL-23 (refs 7, 8, 9). ILC3 cells can be divided into subpopulations by their expression of CD4 and NKp46 (encoded by Ncr1) receptors, such as CD4+ ILC3s, NKp46+ ILC3s and CD4−NKp46− ILC3s (DN ILC3s)1,10. ILC3 subsets in the fetal intestines are CD4+ or CD4− lymphoid tissue inducer (LTi) cells, which are necessary for the development of Peyer’s patches (PPs) and lymph nodes11. In addition, ILC3s are central to the defence against bacterial infection in the intestine12,13; NKp46+ ILC3s, for example, specifically generate IL-22 but not IL-17 (refs 14, 15).
ILC3s are enriched in PPs and intestinal lamina propria10. ILC3s, together with other ILCs, are derived from the earliest progenitor cells (αLPs, CXCR+ integrin α4β7-expressing common lymphoid progenitors (CLPs))16, which differentiate into restricted common helper-like innate lymphoid progenitor (CHILP) cells17. Subsequently, downstream precursor ILCPs (common precursor of ILCs) are characterized by expression of transcription factor PLZF and can generate ILC1, ILC2, and ILC3 subsets18. RORγt (encoded by Rorc) drives differentiation of ILC3s from their precursor ILCPs19,20. RORγt deletion causes a complete loss of ILC3s but not ILC1s or ILC2s. In addition, aryl hydrocarbon receptor (AHR) is highly expressed in ILC3s, and is required for their development and maintenance21,22,23. However, the underlying mechanism that control ILC3 development and maintenance are unclear.
WASH (Wiskott-Aldrich syndrome protein (WASP) and SCAR homologue) was originally identified as an actin-nucleating factor belonging to the WASP family24,25, and has an essential role in endosome sorting via promoting tubule fission by Arp2/3 activation. WASH is also located in autophagosomes that modulate autophagy induction24,26. We previously showed that WASH deficiency results in early embryonic lethality at embryonic day 7.5 (ref. 27), and its deletion in the haematopoietic system causes defective blood production of mice28. WASH is located in the nucleus of haematopoietic stem cells (HSCs) and promotes differentiation of haematopoietic stem cells through initiating c-Myc expression. WASH deficient T cells have been reported to have defective proliferation and impaired effector functions29. One study showed that WASH has a critical function in MHCII recycling and efficient priming of T helper cells30. However, whether WASH is involved in the development and maintenance of ILCs is unknown. Here we show that WASH is highly expressed in the nucleus of ILC3s, and associates with Arid1a to activate AHR expression. WASH-mediated AHR expression is required for the maintenance of NKp46+ ILC3s, and has a critical function in their effector functions.
Results
WASH deficiency impairs the maintenance of NKp46+ ILC3s
We previously demonstrated that WASH is highly expressed in the nucleus of long-term hematopoietic stem cells (LT-HSCs)28. Conditional WASH deletion in the hematopoietic system causes defective blood production of the host 8 weeks after WASH deletion, leading to severe cytopenia and rapid anemia. To further explore whether WASH participates in the development and maintenance of ILCs in the intestine, we assayed compositions of ILCs in Washflox/floxMx1-Cre mice (gene Washc1 is referred to here as Wash) 3 weeks post poly(I:C) administration, meanwhile, bone marrow (BM) hematopoiesis was not affected by WASH deletion. We noticed that WASH deletion reduced the number of ILC3s in the intestine, but not ILC1s or ILC2s (Fig. 1a,b; Supplementary Fig. 1a,b). Of note, WASH deletion caused a greater loss of NKp46 expressing ILC3s (NKp46+ ILC3s), but no impact on CD4+ ILC3s or CD4–NKp46– ILC3s (DN ILC3s) (Fig. 1b; Supplementary Fig. 1c). We also tested progenitors of ILC3s in the BM of Washflox/floxMx1-Cre mice. We observed that WASH deletion did not affect cell numbers of their progenitors such as αLPs, CHILPs and ILCPs (Fig. 1c; Supplementary Fig. 1d–g). These observations suggest that WASH deficiency reduces the number of NKp46+ ILC3s rather than other ILC cell subpopulations.
We next examined mRNA expression levels of WASH in ILC subsets. We found that WASH was actually expressed in all ILC populations and displayed a highest level in NKp46+ ILC3s (Supplementary Fig. 1h,i). We then crossed Washflox/flox mice with Ncr1-Cre mice31 to generate mice with conditional deletion of WASH in NKp46+ ILC3s (Fig. 1d). We observed that WASH deficiency did not affect the number of cryptopatches (Fig. 1e), suggesting that WASH is not necessary for the development of LTi cells. Since ILC3s are enriched in Peyer’s patches and intestinal lamina propria10, we then isolated lamina propria lymphocyte (LPL) populations from small and large intestines to test constitutions of ILCs. We noticed that NKp46+ ILC3s were remarkedly declined in the small intestine of Washflox/floxNcr1-Cre mice compared with that of Washflox/flox wild type (WT) control mice (Fig. 1f; Supplementary Fig. 1j). However, other ILC3 subsets, including CD4–NKp46– ILC3s (DN ILC3s) and CD4+ ILC3s were unchangeable in Washflox/floxNcr1-Cre mice (Fig. 1f; Supplementary Fig. 1j). Similar results were achieved in both large intestines and Peyer’s patches of Washflox/floxNcr1-Cre mice. Given that Ncr1-Cre also induced WASH deletion in NKp46 expressing ILC1s, we then examined cell numbers of ILC1s in intraepithelial lymphocyte (IEL) populations from small intestines. Notably, WASH deletion in ILC1s did not impact cell numbers of this subset, suggesting that WASH is not required for the development and maintenance of ILC1s.
RORγt (encoded by Rorc) is involved in the development of all ILC3 subsets11,32. To further validate the specific role of WASH in the maintenance of NKp46+ ILC3s, we generated Washflox/floxRorc-Cre mice by crossing Washflox/flox mice with Rorc-Cre mice to conditionally delete WASH in ILC3s (Fig. 1g). We observed that WASH deletion in ILC3s did not affect numbers of cryptopatches (Fig. 1h), confirming that WASH deficiency does not impact the development and maintenance of LTi cells. As expected, Washflox/floxRorc-Cre mice significantly reduced cell numbers of NKp46+ ILC3s, but not DN ILC3s or CD4+ ILC3s (Fig. 1i; Supplementary Fig. 1k). Moreover, WASH deficiency had no significant effect on number changes of RORγt+ T cells (Supplementary Fig. 1l). Taken together, we conclude that WASH deficiency impairs the maintenance of NKp46+ ILC3s, but not their development.
Of note, Washflox/floxNcr1-Cre mice did not impact the apoptosis of all ILC3 subsets (Supplementary Fig. 1m). To determine whether WASH affected the expansion of ILC3s, we then analysed cell cycle statuses of ILC3s. We found that Wash−/− NKp46+ ILC3s maintained in a resting state compared with those of Wash+/+ NKp46+ ILC3s (Fig. 1j). Similar results were obtained from WASH deficient NKp46+ ILC3s in Washflox/floxRorc-Cre mice. Of note, WASH deficient mice did not cause susceptible change to Citrobacter rodentium infection (Supplementary Fig. 1n). Finally, WASH deficiency did not affect cell numbers of liver NK cells or NKp46+RORγt− cells in the intestine (Supplementary Fig. 2a,b). Altogether, WASH maintains the cell pool of NKp46+ ILC3 population via the regulation of cell expansion.
WASH intrinsically maintains NKp46+ ILC3s
To examine whether WASH intrinsically affected the maintenance of NKp46+ ILC3s, we transplanted Washflox/floxNcr1-Cre BM cells with an equal number of CD45.1 WT BM cells into lethally irradiated CD45.1 recipient mice for 8 weeks, followed by examination of donor chimerism of ILCs post BM reconstitution (Supplementary Fig. 2c). ILC3s were significantly reduced in Washflox/floxNcr1-Cre BM cells transferred mice compared with those transferred with Washflox/flox control cells. By contrast, ILC1s and ILC2s did not change in Washflox/floxNcr1-Cre BM cells transferred mice compared with those transferred with Washflox/flox control cells. Additionally, we found that NKp46+ ILC3s derived from Washflox/floxNcr1-Cre donor cells were dramatically decreased in small intestines (Fig. 2a), large intestines (Fig. 2b) and Peyer’s patches compared with those derived from Washflox/flox donor cells. However, DN ILC3s or CD4+ ILC3s derived from Washflox/floxNcr1-Cre donor cells were unchangeable (Fig. 2a,b). In addition, NKp46+ ILC3s derived from Washflox/floxNcr1-Cre donor cells sustained in a resting state compared with those from Washflox/flox donor cells (Fig. 2c; Supplementary Fig. 2d). Similar results were achieved by competitively transferring Washflox/floxRorc-Cre BM cells or Washflox/flox BM cells into lethally irradiated CD45.1 recipient mice (Supplementary Fig. 2e–g; Fig. 2d,e). These observations indicate that WASH deletion intrinsically affects the maintenance of NKp46+ ILC3 cells.
WASH promotes AHR expression in NKp46+ ILC3s
To explore the molecular mechanism by which WASH regulated the maintenance of NKp46+ ILC3s, we screened key transcription factors and surface markers involved in development and maintenance of ILCs. We noticed that Ahr was dramatically reduced in WASH deleted NKp46+ ILC3s (Fig. 3a). We isolated ILC subsets from small intestines of Washflox/floxRorc-Cre and Washflox/flox mice and examined AHR expression. Indeed, Ahr was highly expressed in NKp46+ ILC3s derived from Washflox/flox mice (Fig. 3b), with a modest expression in DN ILC3s and CD4+ ILC3s. These results were further validated by immunoblotting (Supplementary Fig. 2h). Notably, WASH knockout specifically impeded AHR expression in NKp46+ ILC3s, but not in DN ILC3s or CD4+ ILC3s (Fig. 3b), suggesting WASH is involved in the regulation of AHR expression in NKp46+ ILC3s. Through chromatin immunoprecipitation (ChIP) assays, we found that WASH bound to Ahr promoter (−400 to −200) in NKp46+ ILC3s (Fig. 3c), but not in DN ILC3s or CD4+ ILC3s (Fig. 3d). WASH deficiency markedly suppressed Ahr transcription in NKp46+ ILC3s by a nuclear run-on assay, but not in DN ILC3s or CD4+ ILC3s (Fig. 3e). We then transplanted WASH overexpressing BM cells together with recipient BM cells into lethally irradiated CD45.1 recipient mice for reconstitution assays. We observed that WASH overexpression augmented Ahr transcription in NKp46+ ILC3s, but not in DN ILC3s or CD4+ ILC3s (Fig. 3f), suggesting other factors than WASH may be required for Ahr expression in DN ILC3s or CD4+ ILC3s. These data indicate that WASH promotes AHR expression in NKp46+ ILC3s through association with its promoter.
We previously showed that WASH acts as a transcription associating factor to promote transcription of target genes via its VCA domain28. We next wanted to determine whether the VCA domain of WASH was required for Ahr transcription. We then transfected full-length WASH (WASH(FL)) or VCA truncated WASH (WASH(ΔVCA)) into pGL3-AHR expressing NK92 cells for luciferase assays. Consistent with our previous results, WASH(ΔVCA) abrogated Ahr activation, whereas WASH(FL) was able to activate Ahr transcription (Fig. 3g). The actin nucleation inhibitor cytochalasin D blocked Ahr activation (Fig. 3g). Furthermore, WASH was co-localized with Ahr promoter in NKp46+ ILC3s by fluorescence staining (Fig. 3h). Furthermore, WASH deficiency repressed the acetylation of H3K9K14 and the methylation of H3K4 on Ahr promoter (Fig. 3i,j), both of which are hallmarks of active gene transcription. Additionally, WASH knockout also made Ahr promoter more resistant to DNase I digestion (Fig. 3k). Consistently, the Ahr promoter region accumulated more repressive histone markers in WASH deficient NKp46+ ILC3s (Fig. 3l). Finally, Ahr activation was remarkably suppressed in WASH deficient NKp46+ ILC3s (Fig. 3m). These observations confirm that WASH promotes Ahr transcription.
To further validate that WASH regulated the maintenance of NKp46+ ILC3s via AHR, we rescued AHR expression in WASH deficient cells by transplanting AHR overexpressing Washflox/floxRorc-Cre BM cells together with recipient BM cells into lethally irradiated recipient mice (Supplementary Fig. 3a). Consequently, AHR overexpression rescued cell numbers of NKp46+ ILC3s reduced by WASH knockout (Supplementary Fig. 3b). Additionally, AHR restoration in NKp46+ ILC3s maintained more cycling cells compared with those with empty vector expression (Supplementary Fig. 3c). We also rescued WASH(FL) or WASH(ΔVCA) in WASH deficient NKp46+ ILC3s by transplanting WASH(FL) or WASH(ΔVCA) overexpressing Washflox/floxRorc-Cre BM cells together with recipient BM cells into lethally irradiated recipient mice (Supplementary Fig. 3d). WASH(FL) overexpression was able to restore the cell number of NKp46+ ILC3s reduced by WASH deletion, whereas WASH(ΔVCA) has no such effect (Supplementary Fig. 3e). Consistently, WASH restoration promoted AHR expression in NKp46+ ILC3s (Supplementary Fig. 3f), and sustained more cycling cells (Supplementary Fig. 3g). Of note, anti-Arid1a antibody could precipitate Ahr promoter in anti-WASH antibody precipitates (Supplementary Fig. 3h). Anti-WASH antibody could also precipitate the Ahr promoter in anti-Arid1a antibody precipitates (Supplementary Fig. 3i), suggesting that WASH and Arid1a together bind to the Ahr promoter region. Furthermore, WASH associated with Arid1a only in the NKp46+ ILC3s (Supplementary Fig. 3j), suggesting that the Ahr expression is differentially regulated among different ILC3 subsets. Altogether, WASH maintains the cell pool of NKp46+ ILC3s via promoting AHR expression.
WASH associates with Arid1a to promote Ahr transcription
To elucidate how WASH promoted Ahr transcription in NKp46+ ILC3s, we screened a cDNA library using WASH as bait via a yeast two-hybrid system. We identified Arid1a as a new interactor of WASH (Fig. 4a). Arid1a belongs to the BRG1-associated factor (BAF) complex that is involved in nucleosome remodelling and gene transcription33. Recombinant WASH could precipitate Arid1a from LPL lysates of small intestine (Fig. 4b). Moreover, anti-WASH antibody could precipitate Arid1a from lysates of NKp46+ ILC3s (Fig. 4c), confirming the interaction of WASH with Arid1a. By contrast, we noticed that Arid1a signals could not be picked up by anti-Arid1a antibody in anti-WASH precipitates derived from Arid1a deleted ILC3s lysates (Fig. 4c). These results validated the specificity of these two antibodies we used. Through domain mapping, we identified that two fragments of Arid1a (aa 968–1,484 and aa 1,935–2,283) were required for WASH binding (Fig. 4d). Of note, Arid1a knockdown abolished the interaction between WASH and other components of BAF complex (Fig. 4e), suggesting that WASH associates with BAF complex via Arid1a interaction. Similar to WASH, Arid1a bound to the same region of Ahr promoter in NKp46+ ILC3s (Fig. 4f). Additionally, other components of BAF complex were also recruited to Ahr promoter (Fig. 4g). These data suggest that WASH may recruit BAF complex to Ahr promoter for enhancing its transcription.
We generated Arid1aflox/floxRorc-Cre mice by crossing Arid1aflox/flox mice with Rorc-Cre mice. Arid1a was completely deleted in ILC3s (Fig. 4h). Through nuclear run-on assays, we noticed that Arid1a knockout repressed Ahr transcription in NKp46+ ILC3s (Fig. 4i), but not in DN ILC3s or CD4+ ILC3s. We next transplanted Arid1a overexpressing BM cells together with recipient BM cells into lethally irradiated recipient mice. We observed that Arid1a overexpression caused elevated expression of AHR in NKp46+ ILC3s, but not in DN ILC3s or CD4+ ILC3s (Fig. 4j). We observed that Arid1a was expressed in three subsets of ILC3s (Fig. 5a). Moreover, Arid1a deficiency suppressed the acetylation of H3K9K14 and the methylation of H3K4 on Ahr promoter (Fig. 5b,c). Arid1a knockout also made Ahr promoter more resistant to DNase I cleavage (Fig. 5d), suggesting an inaccessible state of Ahr promoter for transcriptional activation. We next transfected full-length Arid1a (Arid1a(FL)) or two Arid1a mutants lacking WASH binding domains into pGL3-AHR expressing WASH silenced NK92 cells, followed by examination of Ahr activation through luciferase assays. We observed that Arid1a mutants failed to activate Ahr transcription in both shCtrl treated and WASH silenced cells (Fig. 5e). By contrast, Arid1a(FL) could activate Ahr transcription in shCtrl treated cells, but not in WASH silenced cells (Fig. 5e). These observations suggest that Arid1a promotes Ahr transcription in a WASH-dependent manner.
WASH knockout abrogated the co-localization of Arid1a with Ahr promoter in NKp46+ ILC3s by fluorescence imaging (Fig. 5f). In parallel, WASH deletion impaired the recruitment of Arid1a and BAF complex to Ahr promoter by ChIP assays (Fig. 5g,h). Finally, in WASH silenced cells, Arid1a mutants failed to bind Ahr promoter (Fig. 5i). Taken together, we conclude that WASH recruits the BAF complex to promote AHR expression in NKp46+ ILC3s.
Arid1a maintains NKp46+ ILC3s through AHR ILC3s
We next examined ILC3 subsets in Arid1aflox/floxRorc-Cre and Arid1aflox/flox mice. We observed that Arid1aflox/floxRorc-Cre mice showed reduced numbers of NKp46+ ILC3s, but not other subsets of ILC3s (Fig. 6a), these observations were similar to those of Washflox/floxRorc-Cre mice. In addition, Arid1a deficiency did not affect the apoptosis of NKp46+ ILC3s either. We found that Arid1a deficient NKp46+ ILC3s derived from Arid1aflox/floxRorc-Cre mice incorporated much less BrdU than those from Arid1aflox/flox mice (Fig. 6b). In addition, Arid1a−/− NKp46+ ILC3s maintained in a resting state compared with those of Arid1a+/+ NKp46+ ILC3s (Fig. 6c). Similar results were obtained when we reconstituted recipient mice with Arid1a deficient BM cells. We next overexpressed full length or mutant Arid1a in Arid1aflox/floxRorc-Cre BM cells and then transplanted these BM cells together with recipient BM cells into lethally irradiated recipient mice. We found that overexpression of two Arid1a mutants lacking WASH binding ability in Arid1aflox/floxRorc-Cre BM cells failed to rescue donor reconstitution ratios of NKp46+ ILC3s caused by Arid1a deficiency (Fig. 6d). By contrast, overexpression of full length Arid1a in Arid1aflox/floxRorc-Cre BM cells could rescue donor reconstitution rates of NKp46+ ILC3s (Fig. 6d). Consistently, Arid1a restoration promoted AHR expression in NKp46+ ILC3s (Fig. 6e), and sustained more cycling cells of these NKp46+ ILC3s (Fig. 6f). These results indicate that Arid1a is essential for the maintenance of NKp46+ ILC3s.
We next wanted to determine whether WASH and Arid1a exerted a synergistic effect. We generated WASH and Arid1a double knockout (DKO) mice through crossing Arid1aflox/floxRorc-Cre mice with Washflox/flox mice. We noticed that DKO mice did not affect numbers of Peyer’s patches. Consistently, DKO mice markably decreased cell numbers of NKp46+ ILC3s as well as IL-22 expressing ILC3s (Supplementary Fig. 4a, b). In addition, DKO mice dramatically reduced AHR expression in NKp46+ ILC3s (Supplementary Fig. 4c), accompanied with fewer cycling cells (Supplementary Fig. 4d). Finally, DKO mice did not impact apoptosis of NKp46+ ILC3s (Supplementary Fig. 4e). These observations indicate that WASH and Arid1a exert synergistc effect on Ahr expression.
We next rescued AHR in Arid1a KO or DKO NKp46+ ILC3s by transplanting AHR overexpressing Arid1aflox/floxRorc-Cre or Washflox/floxArid1aflox/floxRorc-Cre BM cells together with recipient BM cells into lethally irradiated recipient mice for reconstitution assays (Supplementary Fig. 4f). AHR restoration rescued donor reconstitution ratios of NKp46+ ILC3s reduced by Arid1a KO or DKO of WASH with Arid1a (Supplementary Fig. 4g), and maintained more cycling cells (Supplementary Fig. 4h). We next transplanted Arid1a-rescued Arid1aflox/floxRorc-Cre or Washflox/floxArid1aflox/floxRorc-Cre BM cells together with recipient BM cells into lethally irradiated recipient mice. Consistently, Arid1a overexpression could indeed rescue donor reconstitution ratios of NKp46+ ILC3s reduced by Arid1a KO or DKO (Supplementary Fig. 5a,b), and sustained more cycling cells (Supplementary Fig. 5c). Consequently, Arid1a overexpression augmented AHR expression in Arid1a−/− NKp46+ ILC3s, but not in Wash−/−Arid1a−/− NKp46+ ILC3s (Supplementary Fig. 5d). In addition, AHR ligand FICZ engagement sustained more cycling NKp46+ ILC3s in WT ILC3s, but not in Arid1a−/− or in Wash−/−Arid1a−/− ILC3s (Supplementary Fig. 5e). By contrast, AHR antagonist treatment rendered NKp46+ ILC3s in resting state even in WT ILC3s (Supplementary Fig. 5e). Altogether, WASH-mediated AHR expression is required for the maintenance of NKp46+ ILC3s.
Discussion
Innate lymphoid cells (ILCs) are a distinct arm of the innate immune system, and can directly communicate with other hematopoietic and non-hematopoietic cells to regulate immunity, inflammation and tissue homeostasis34. However, how these ILC lineages develop and maintain remains largely unknown. In this study, we show that WASH is highly expressed in the nucleus of ILC3s. WASH deletion impairs the maintenance of NKp46+ ILC3s but not other ILC subsets. In NKp46+ ILC3s, WASH recruits Arid1a to Ahr promoter to activate AHR expression. WASH-mediated AHR expression is required for the maintenance of NKp46+ ILC3s. WASH deletion in ILC3s reduced the number of NKp46+ ILC3s. Notably, double knockout of WASH and Arid1a impedes AHR expression and NKp46+ ILC3s maintenance.
All ILC subsets are derived from common lymphoid progenitors (CLPs), which also differentiate into T and B cells17,34. The earliest progenitor cells specific to ILCs are CXCR+ integrin α4β7–expressing CLPs, referred to as α–lymphoid precursor (αLP) cells, which give rise to ILC1, ILC2, ILC3 and conventional NK cells (cNKs)35,36. The common progenitor to all ILC lineages (CHILP) is identified as its Lin−CD127+Id2+CD25−α4β7+ phenotype and differentiates to all ILC subsets, but not cNKs17. The common precursor to ILCs (ILCP) is defined by expression of transcription factor PLZF and gives rise to ILC1, ILC2, and ILC3 subpopulations18. We previously reported that conditional deletion of WASH in the hematopoietic system impairs the differentiation of HSCs and causes defective blood production of the host28. In this study, we show that WASH deletion in the hematopoietic system does not impact the development of progenitors of ILCs, including αLP, CHILP and ILCP cells. However, WASH deficiency only affects the maintenance of NKp46+ ILC3 subsets, but not other ILC lineages or ILC3 subpopulations. Our observations suggest that the development and maintenance of different ILC subsets utilize different intrinsic checkpoints.
Group 3 ILCs (ILC3) are defined by their expression of RORγt, including CCR6+ lymphoid tissue inducer (LTi) cells and CCR6− ILC3 lineage15,37. Through expression of lymphotoxin (LT) α1β2, LTi cells induce the development of lymph nodes and Peyer’s patches through LTβ receptor (LTβR) signalling38,39. Some CCR6− ILC3s express a natural cytotoxicity-triggering receptor (NCR): NKp46 in mice32 and NKp44 in humans40, and eventually develop into NKp46+ ILC3s. Unlike LTi cells, CCR6− ILC3s are developed from PLZF+ ILCP cells18, and they proliferate largely in mice mainly after birth to 3–4 weeks of age41. NKp46+ ILC3s uniquely produces only IL-22, not IL-17 (refs 14, 15), and have the potential to differentiate into IFN-γ producing ILC1s17. Here we show that WASH deletion only impairs the maintenance of NKp46+ ILC3s and reduces IL-22 production. However, WASH deletion did not change the IFN-γ secretion, suggesting that WASH specifically controls the maintenance of NKp46+ ILC3s, but not IFN-γ producing ILC1s.
WASH, a member of WASP family, plays a critical role in endosomal trafficking via its actin nucleation function24,25,42. We previously showed that WASH is localized at autophagosomes of mouse embryonic fibroblasts (MEFs) and regulates autophagy induction26,27. We also found that WASH is constitutively expressed in the blood cells and mainly resides in the nucleus of long-term hematopoietic stem cells (LT-HSCs)28. WASH deletion breaks the balance of self-renewal and differentiation of HSCs. We demonstrated that WASH promotes the differentiation of LT-HSCs through association with the NURF complex, which activates c-Myc transcription28. Chromatin remodelling complexes are involved in nucleosome sliding, dissociation, or replacement using the energy derived from ATP hydrolysis43,44. Herein we show that WASH is located in the nucleus of NKp46+ ILC3s and associates with the BAF chromatin remodelling complex through direct interaction with Arid1a. Arid1a, also known as BAF250a, is a major component of BAF complex45. Arid1a deficiency impairs the pluripotency and self-renewal of embryonic stem (EC) cells, and results in early embryonic lethality46. Here we showed that WASH is preferentially highly expressed in NKp46+ ILC3s. High nuclear levels of WASH in NKp46+ ILC3s could be able to recruit the BAF complex to Ahr promoter for promoting its transcription. WASH-mediated AHR expression controls the maintenance of NKp46+ ILC3 cell pool of the host. However, why WASH is preferentially expressed in NKp46+ ILC3s needs to be further investigated.
AHR is expressed in many cell types and thus exerts pleiotropic effects by integrating with other signalling pathways. AHR deficient mice frequently died after birth or exhibited a slower growth rate in the first few weeks of life47. AHR signalling is implicated in a variety of immune responses from different cell types. Especially, AHR plays a critical role at mucosal interfaces where the host encounters other living entities and environmental agents. Of note, ILCs are preferentially distributed at mucosal surfaces, where they can perceive changes in the surrounding microenvironment primarily via cytokine receptor signalling14,23. ILC3s express high levels of AHR and sensitively respond to AHR signalling. AHR signalling is not only needed for IL-22 production by ILC3s, but is necessary for their development and/or maintenance21,22,23. AHR deficient mice displayed reduced numbers of ILC3s in small intestines and in Peyer’s patches. Here we show that WASH-mediated AHR expression controls the maintenance of NKp46+ ILC3s, but not other ILC lineages. Additionally, WASH-mediated AHR expression has no significant impact on the development of NKp46+ ILC3s. Consequently, decreased numbers of NKp46+ ILC3s substantially reduce production of IL-22. In sum, WASH-mediated AHR expression is indispensable for the maintenance of NKp46+ ILC3s in the intestine.
Methods
Antibodies and reagents
A rabbit polyclonal antibody against WASH was generated from the VCA domain of WASH protein as described previously27. Commercial antibodies: Antibodies against CD3 (17A2), CD4 (GK1.5), CD19 (1D3), CD127 (A7R34), c-Kit (2B8), Sca-1 (D7), CD25 (PC61.5), CD11b (M1/70), CD11c (N418), Gr1 (RB6-8C5), F4/80 (BM8), Ter119 (TER-119), CD27 (LG.7F9), CD90 (HIS51), CD45.2 (104), RORγt (AFKJS-9), NKp46 (29A1.4), CD244 (C9.1), Flt3 (A2F10), Integrinα4β7 (DATK32), CD45.1 (A20), NK1.1 (PK136), IL-22 (IL22JOP), Thy1.2 (30-H12), and PLZF (Mags.21F7) were purchased from eBiosciences (San Diego, USA) and used in a 1:100 diution for flow cytometric staining. Anti-Arid1a (D2A8U), anti-BAF155 (D7F8S), anti-BAF170 (D8O9V) and anti-SNF5 (D8M1X) antibodies were from Cell Signaling Technology and used in a 1:2,000 dilution for werstern blotting and in a 1:500 dilution for immunofluorescence staining. Anti-GST (6G9C6) and anti-β-actin (SP124) antibodies were from Sigma-Aldrich and used in a 1:2,000 dilution for western blotting. Donkey anti-rabbit IgG secondary antibodies conjugated with Alexa-488, Alexa-594 or Alexa-405 were purchased from Molecular Probes. Donkey anti-mouse IgG secondary antibodies conjugated with Alexa-488 or Alexa-594 were from Molecular Probes. Anti-HA and HRP-conjugated secondary antibodies were from Santa Cruz. BrdU, DAPI and Cytochalasin D were from Sigma-Aldrich. FICZ was from Enzo Life Sciences. AHR antagonist was from Millipore. IL-22 detection kit was from eBioscience.
Cell culture and transfection
NK cell line NK92 (ATCC: CRL-2407) was cultured in Alpha Minimum Essential medium (α-MEM) containing 2 mM L-glutamine, 1.5 g l−1 sodium bicarbonate, 0.1 mM 2-mercaptoethanol, 0.02 mM folic acid, 500 U ml−1 recombinant IL-2, 12.5% horse serum and 12.5% FBS. For NK92 transfection, cells (1 × 106) were resuspended in 100 μl Nucleofector Solution buffer (Lonza) containing 5 μg DNA, followed by transfection using the Nucleofector Program Y-001 on Amaxa nucleofector II device (Lonza). Cells were recovered in α-MEM for 6 h, followed by flow cytometric sorting for viable cells and further culture48. For BM cell transfection, pSIN-EF2-IRES-EGFP lentiviral vectors carrying the indicated genes were transfected into HEK293T cells (maintained by our laboratory) together with psPAX2 and pMD2.G, followed by concentration through ultracentrifugation on 50,000 g for 2 h. Pellets of lentivirus were resuspended in serum-free α-MEM media. Donor BM cells were infected with lentiviruses by centrifugation at 500 g for 1.5 h in the presence of 8 μg ml−1 Polybrene (Sigma-Aldrich) and then incubated at 37 °C for 18 h, followed by sorting for GFP+ cells that were used for transplantation into lethally irradiated recipient mice49.
Animals
Mouse experiments complied with ethical regulations and were approved by the Institutional Animal Care and Use Committees at the Institute of Biophysics, Chinese Academy of Sciences. Washflox/flox mice were generated as previously described27. Arid1aflox/flox mice were provided by Dr. Ze-Guang Han (Chinese National Human Genome Center, Shanghai, China). Ncr1-Cre mice were generated by Beijing Biocytogen (Beijing, China). Rorc-Cre mice and RORγt-GFP mice were purchased from Jackson Laboratory. All mice were in a C57/BL6 background. Female mice at an age of 8 weeks were used in this study unless mentioned in the text.
C. rodentium infection
C. rodentium was a gift from Dr Baoxue Ge (Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences). Mice were orally infected with C. rodentium (2 × 109 per mouse), followed by survival rate and body change examination in the following days. Mice were sacrificed to examine colon pathology and bacterial loads on day 8 post infection.
Intestinal lymphocyte separation and flow cytometry
Intestinal lymphocytes were separated from intestines as described1. Briefly, intestines were cut into pieces with 0.5 cm length post removing fat tissues and Peyer’s patches, followed by digestion in HBSS buffer containing 15 mM HEPES, 5 mM EDTA and 10% FBS. To obtain IELs, intestines were digested in the above buffer on a wheeler for 30 min at 37 °C for three times to collect the detached cells from the intestinal tissues. To obtain LPLs, the remaining intestinal tissues were further digested in HBSS buffer containing 10% FBS, 0.2 mg ml−1 collagenase and 0.2 mg ml−1 DNase I (Sigma-Aldrich). Detached cells were sifted through 50 μm cell strainers for further analysis. For flow cytometry, cells were stained with fluorescence-conjugated primary antibodies for 1 h on ice. Cells were then fixed and permeabilized, followed by nuclear staining of transcription factors. Cells were analysed or sorted by a cell sorter (BD AriaIII). Data were analysed using the FlowJo 7.6.1 software.
BrdU incorporation and cell cycle analysis
Mice were intraperitoneally injected with BrdU (7 mg kg−1 body weight) for 16 h, followed by flow cytometry of BrdU signals in LT-HSCs. For staining BrdU in the nucleus, cells were fixed and permeabilized in fixation/permeabilization buffer (eBioscience) according to manufacturer’s instructions. Permeabilized cells were stained with fluorophore-conjugated anti-BrdU antibody for 2 h, and washed with diluted permeabilization buffer for three times. For cell cycle analysis using Hoechst/Pyronin Y staining strategy, live cells were harvested from RORγt-GFP reporter mice and resuspended in 200 μl IMDM medium containing 10 μg ml−1 Hoechst33342 for 45 min at 37 °C, followed by addition of Pyronin Y to a final concentration of 0.5 μg ml−1 for 15 min at 37 °C.
Histology
Colons were fixed in 4% paraformaldehyde (PFA) (Sigma-Aldrich) for 12 h. Fixed tissues were washed for twice using 75% ethanol and embedded in paraffin, followed by sectioning and staining with haematoxylin and eosin according to standard procedures.
Bone marrow transplantation
Bone marrow transplantation (BMT) was performed as previously described50. Briefly, 2 × 106 BM cells were co-transplanted with 2 × 106 WT CD45.1 BM cells into lethally irradiated mice. For knockdown or overexpression, 107 BM cells were infected with lentiviruses carrying shRNAs or overexpression sequences, followed by culture in HSC culture medium StemPro-34 supplemented with cytokines for 36 h. GFP positive LT-HSCs were sorted through flow cytometer, followed by BMT of 2 × 106 BM cells with 2 × 106 WT CD45.1 BM cells into lethally irradiated mice. CD45.2 and CD45.1 C57BL/6 mice were bred in SPF conditions. Mouse experiments complied with ethical regulations and were approved by the Institutional Animal Care and Use Committees at the Institute of Biophysics, Chinese Academy of Sciences.
Immunofluorescence assay
Immunostaining was performed as previously described51. Briefly, sorted cells were sticked on 0.01% poly-L-Lysine treated coverslips and fixed with 4% PFA for 10 min, followed by permeabilization with 0.5% Triton X-100 for 20 min at room temperature (RT). Primary antibodies were added for 2 h at RT post blocking with 10% donkey serum for 30 min. Samples were further stained with Alexa488-, Alexa594- or Alexa405-conjugated secondary antibodies, followed by confocal microscopy (Olympus FV1000).
DNA FISH
Cells were fixed with 4% PFA containing 10% acetic acid for 15 min at room temperature, followed by replacement with 70% ethanol at −20 °C. Cells were then incubated in buffer containing 100 mM Tris-HCl (pH 7.5), 150 mM NaCl, followed by cytoplasm digestion in 0.01% pepsin/0.01 N HCl for 3 min at 37 °C. Cells were further fixed in 3.7% PFA and replaced with ethanol to a final concentration of 100%. Cells were air dried and washed with 2 × SSC, followed by blocking with buffer containing 100 mM Tris-HCl (pH 7.5), 150 mM NaCl, 0.05% Tween 20, 3% BSA for 20 min. Cells were then denatured in 70% formamide/2 × SSC, and incubated with fluorescence-labeled DNA probes overnight. Cells were counterstained with DAPI for nucleus post washing with PBS.
Western blot
Cells were lysed with RIPA buffer (150 mM NaCl, 0.5% sodium deoxycholate, 0.1% SDS, 1% NP40, 1 mM EDTA, 50 mM Tris (pH 8.0)), followed by separation with SDS-PAGE. Samples were then transferred to NC membrane and incubated with primary antibody in 5% BSA. After washing with TBST three times, membranes were incubated with HRP-conjugated secondary antibodies for visualization. 1 × 105 cells were used for a single sample for whole cell blots. See Supplementary Fig. 6 for uncropped blots.
Co-immunoprecipitation assay
For co-IP experiments, 4 × 105 NKp46+ ILC3 cells (pooled from 20 RORγt-GFP reporter mice) were lysed in hypotonic buffer (10 mM HEPES, 1.5 mM MgCl2, 10 mM KCl, 0.5 mM DTT) to remove cytoplasmic contents. Nuclear pellets were lysed in RIPA lysis buffer for 1 h, followed by incubation with anti-WASH antibody for 4 h post preclearance with protein A/G agarose. WASH containing complex was further precipitated by protein A/G agarose. Immunoprecipitates were washed with RIPA buffer, followed by immunoblotting with antibodies against WASH and Arid1a. 4 × 105 Arid1a deleted ILC3 cells sorted from Arid1aflox/floxRorc-Cre mice were also lysed and immunoprecipitated with anti-WASH antibody for co-IP assay as a control.
Chromatin immunoprecipitation
ChIP was performed using MAGnify ChIP kit with manufacturers’ instructions. Briefly, cells sorted from intestines were crosslinked with 1% formaldehyde at 37 °C for 10 min, and then quenched with 0.125 M lysine, followed by swelling in lysis buffer (50 mM Hepes, pH 7.5, 140 mM NaCl, 1% Triton X-100, 0.1% NaDeoxycholate, and Protease Inhibitor Cocktail Set III) for 30 min on ice. Chromatin was sheared to a mean length of 400 bp by sonication. After being de-cross-linked by RNase, proteinase K, and heat, input genomic DNA was precipitated with ethanol and quantified in a GeneQuant 100 spectrophotometer (GE Healthcare). Chromatin was precleared with protein A/G-agarose, followed by incubation with indicated antibodies at 4 °C overnight and further incubation with protein A/G-agarose for 2 h. Beads were washed with washing buffer (10 mM Tris, pH 8.0, 250 mM LiCl, 0.5% NP-40, 0.5% Nadeoxycholate, 1 mM EDTA) three times and eluted with elution buffer (50 mM Tris, pH 8.0, 1% SDS, and 10 mM EDTA). Eluates were de-cross-linked by RNase, proteinase K, and heat, and DNA was extracted with phenolchloroform, followed by ethanol precipitation. For each ChIP experiment, 2 × 104 cells were used. 5% of nuclear extracts served as inputs. Immunoprecipitated DNAs were further analysed by real-time PCR. 2 × 104 NKp46+ ILC3 cells were pooled from 4–10 WT or WASH knockout mice for each group.
RNA interference
RNA interference was performed according to pSUPER system instructions (Oligoengine). pSUPER vectors enclosing target sequences were constructed52. WASH target sequences were: #1, 5′-GCCAGAGCTAGAGAATGAA-3′; #2, 5′-AGCGCAAACTGG AGAAGAA-3′.
RT-PCR assay
Total RNA was extracted from cells using Trizol reagent and cDNA was reverse-transcribed using Superscript II (Invitrogen). RT-PCR was performed using StarScript II Two-step RT-PCR Kit (Genestar) with the following primers: Wash, forward, 5′-CTCCTTGGCCCAGGCTAAG-3′, reverse, 5′-CTGCAGAGCCCGCTCATCCAG-3′; Arid1a, forward, 5′-ATCTTCGCAGCTGCTGACTCC-3′, reverse, 5′-GGCATCCTGGATTC CGACTGAG-3′; Ahr, forward, 5′-AAGAAAGGGAAGGACGGAGC-3′, reverse, 5′-CTGCCC TTTGGCATCACAAC-3′.
Recombinant protein preparation
cDNAs were cloned from a bone marrow cDNA library48. WASH or Arid1a was subcloned to pGEX-6 P-1 for GST-tagged protein expression or to pET-28a for His-tagged protein purification. Plasmids were transformed into E. coli strain BL21 (DE3). DE3 clones were cultured (OD600=0.6), followed by induction with 0.2 mM IPTG at 16 °C for 24 h. Cells were collected and lysed by supersonic, followed by purification through Ni-NTA resins or GST resins. GST-tagged proteins were cleaved by PreScission protease to remove GST tags.
Luciferase assay
Luciferase reporter was constructed as described51. Ahr promoter region (−2,000 to 0) was subcloned into pGL3 vector. Luciferase reporter vectors were cotransfected with pRL-TK (as an internal control reporter vector) into macrophages by electroporation. Luciferase assays were performed with guidelines provided by the manufacturer (Promega). For NK92 transfection, cells (1 × 106) were resuspended in 100 μl Nucleofector Solution buffer (Lonza) containing 5 μg DNA, followed by transfection using the Nucleofector Program Y-001 on Amaxa nucleofector II device (Lonza). Cells were recovered in RPMI1640 media containing 4 mM L-glutamine, 1.5 g l−1 sodium bicarbonate, and 10% heat-inactivated fetal bovine serum (FBS) for 6 h, followed by flow cytometry sorting for viable cells.
Nuclear run-on assay
Sorted cells were harvested in buffer containing 150 mM KCl, 10 mM Tris-HCl, 4 mM MgOAc with pH 7.4, followed by centrifugation to collect cell pellets. Pellets were lysed in buffer containing 150 mM KCl, 10 mM Tris-HCl, 4 mM MgOAc, and 0.5% NP-40, followed by sucrose density gradient centrifugation to prepare crude nuclei. Crude nuclei were incubated with 10 mM ATP, CTP, GTP, BrUTP and RNase inhibitor at 28 °C for 5 min. RNAs were extracted using TRIzol reagent with manufacturer’s guidelines, followed by DNA digestion with DNase I. RNA transcripts were immunoprecipitated with antibody against BrdU, followed by reverse transcription and RT-PCR analysis. 5 × 104 cells were used for a single sample.
DNaseI accessibility assay
Nuclei were purified from cells according to the manufacturer’s protocol with Nuclei Isolating Kit (NUC101-1KT, Sigma-Aldrich). Then nuclei were resuspended with DNase I digestion buffer and treated with indicated units of DNase I (Sigma-Aldrich) at 37°C for 5 min. 2 × DNase I stop buffer (20 mM Tris Ph 8.0, 4 mM EDTA, 2 mM EGTA) was added to stop reactions. DNA was extracted and examined by PCR.
Yeast two-hybrid screening
Yeast two-hybrid screening was performed as described27. Briefly, WASH was cloned into pGBKT7 vector (BD-WASH). Yeast AH109 cells were transfected with BD-WASH and plasmids containing a mouse BM cDNA library (Clontech/Takara) and then plated on selective SD medium. Selected clones were isolated and sequenced. X-α-gal assay was carried out following manufacturer’s instructions.
Statistical analysis
Student’s t-test was used as statistical analysis by using Microsoft Excel53.
Data availability
The authors declare that the data supporting the findings of this study are available within the article and its supplementary information files.
Additional information
How to cite this article: Xia, P et al. WASH maintains NKp46+ ILC3 cells by promoting AHR expression. Nat. Commun. 8, 15685 doi: 10.1038/ncomms15685 (2017).
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References
Ebihara, T. et al. Runx3 specifies lineage commitment of innate lymphoid cells. Nat. Immunol. 16, 1124–1133 (2015).
Constantinides, M. G. et al. PLZF expression maps the early stages of ILC1 lineage development. Proc. Natl Acad. Sci. USA 112, 5123–5128 (2015).
Bernink, J. H. et al. Interleukin-12 and -23 control plasticity of CD127(+) group 1 and group 3 innate lymphoid cells in the intestinal lamina propria. Immunity 43, 146–160 (2015).
Seehus, C. R. et al. The development of innate lymphoid cells requires TOX-dependent generation of a common innate lymphoid cell progenitor. Nat. Immunol. 16, 599–608 (2015).
Moro, K., Ealey, K. N., Kabata, H. & Koyasu, S. Isolation and analysis of group 2 innate lymphoid cells in mice. Nat. Protoc. 10, 792–806 (2015).
Brestoff, J. R. et al. Group 2 innate lymphoid cells promote beiging of white adipose tissue and limit obesity. Nature 519, 242–246 (2015).
Magri, G. & Cerutti, A. Role of group 3 innate lymphoid cells in antibody production. Curr. Opin. Immunol. 33, 36–42 (2015).
Hepworth, M. R. et al. Group 3 innate lymphoid cells mediate intestinal selection of commensal bacteria-specific CD4(+) T cells. Science 348, 1031–1035 (2015).
Yagi, R. et al. The transcription factor GATA3 is critical for the development of all IL-7Ralpha-expressing innate lymphoid cells. Immunity 40, 378–388 (2014).
Serafini, N. et al. Gata3 drives development of RORgammat+ group 3 innate lymphoid cells. J. Exp. Med. 211, 199–208 (2014).
Eberl, G. et al. An essential function for the nuclear receptor RORgamma(t) in the generation of fetal lymphoid tissue inducer cells. Nat. Immunol. 5, 64–73 (2004).
Guo, X. et al. Innate Lymphoid cells control early colonization resistance against intestinal pathogens through ID2-dependent regulation of the microbiota. Immunity 42, 731–743 (2015).
Guo, X. et al. Induction of innate lymphoid cell-derived interleukin-22 by the transcription factor STAT3 mediates protection against intestinal infection. Immunity 40, 25–39 (2014).
Diefenbach, A., Colonna, M. & Koyasu, S. Development, differentiation, and diversity of innate lymphoid cells. Immunity 41, 354–365 (2014).
Klose, C. S. et al. A T-bet gradient controls the fate and function of CCR6-RORgammat+ innate lymphoid cells. Nature 494, 261–265 (2013).
De Obaldia, M. E. & Bhandoola, A. Transcriptional regulation of innate and adaptive lymphocyte lineages. Annu. Rev. Immunol. 33, 607–642 (2015).
Klose, C. S. et al. Differentiation of type 1 ILCs from a common progenitor to all helper-like innate lymphoid cell lineages. Cell 157, 340–356 (2014).
Constantinides, M. G., McDonald, B. D., Verhoef, P. A. & Bendelac, A. A committed precursor to innate lymphoid cells. Nature 508, 397–401 (2014).
Montaldo, E. et al. Human RORgammat(+)CD34(+) cells are lineage-specified progenitors of group 3 RORgammat(+) innate lymphoid cells. Immunity 41, 988–1000 (2014).
Sanos, S. L. et al. RORgammat and commensal microflora are required for the differentiation of mucosal interleukin 22-producing NKp46+ cells. Nat. Immunol. 10, 83–91 (2009).
Kiss, E. A. et al. Natural aryl hydrocarbon receptor ligands control organogenesis of intestinal lymphoid follicles. Science 334, 1561–1565 (2011).
Lee, J. S. et al. AHR drives the development of gut ILC22 cells and postnatal lymphoid tissues via pathways dependent on and independent of Notch. Nat. Immunol. 13, 144–151 (2012).
Qiu, J. et al. The aryl hydrocarbon receptor regulates gut immunity through modulation of innate lymphoid cells. Immunity 36, 92–104 (2012).
Derivery, E. et al. The Arp2/3 activator WASH controls the fission of endosomes through a large multiprotein complex. Dev. Cell 17, 712–723 (2009).
Gomez, T. S. & Billadeau, D. D. A FAM21-containing WASH complex regulates retromer-dependent sorting. Dev. Cell 17, 699–711 (2009).
Xia, P. et al. RNF2 is recruited by WASH to ubiquitinate AMBRA1 leading to downregulation of autophagy. Cell Res. 24, 943–958 (2014).
Xia, P. et al. WASH inhibits autophagy through suppression of Beclin 1 ubiquitination. EMBO J. 32, 2685–2696 (2013).
Xia, P. et al. WASH is required for the differentiation commitment of hematopoietic stem cells in a c-Myc-dependent manner. J. Exp. Med. 211, 2119–2134 (2014).
Piotrowski, J. T., Gomez, T. S., Schoon, R. A., Mangalam, A. K. & Billadeau, D. D. WASH knockout T cells demonstrate defective receptor trafficking, proliferation, and effector function. Mol. Cell. Biol. 33, 958–973 (2013).
Graham, D. B. et al. Dendritic cells utilize the evolutionarily conserved WASH and retromer complexes to promote MHCII recycling and helper T cell priming. PLoS ONE 9, e98606 (2014).
Wang, S. et al. FoxO1-mediated autophagy is required for NK cell development and innate immunity. Nat. Commun. 7, 11023 (2016).
Satoh-Takayama, N. et al. Microbial flora drives interleukin 22 production in intestinal NKp46+ cells that provide innate mucosal immune defense. Immunity 29, 958–970 (2008).
Wilson, B. G. & Roberts, C. W. SWI/SNF nucleosome remodellers and cancer. Nat. Rev. Cancer 11, 481–492 (2011).
Artis, D. & Spits, H. The biology of innate lymphoid cells. Nature 517, 293–301 (2015).
Cherrier, M., Sawa, S. & Eberl, G. Notch, Id2, and RORgammat sequentially orchestrate the fetal development of lymphoid tissue inducer cells. J. Exp. Med. 209, 729–740 (2012).
Possot, C. et al. Notch signaling is necessary for adult, but not fetal, development of RORgammat(+) innate lymphoid cells. Nat. Immunol. 12, 949–958 (2011).
McKenzie, A. N., Spits, H. & Eberl, G. Innate lymphoid cells in inflammation and immunity. Immunity 41, 366–374 (2014).
Bouskra, D. et al. Lymphoid tissue genesis induced by commensals through NOD1 regulates intestinal homeostasis. Nature 456, 507–510 (2008).
De Togni, P. et al. Abnormal development of peripheral lymphoid organs in mice deficient in lymphotoxin. Science 264, 703–707 (1994).
Cella, M. et al. A human natural killer cell subset provides an innate source of IL-22 for mucosal immunity. Nature 457, 722–725 (2009).
Sawa, S. et al. Lineage relationship analysis of RORgammat+ innate lymphoid cells. Science 330, 665–669 (2010).
Hao, Y. H. et al. Regulation of WASH-dependent actin polymerization and protein trafficking by ubiquitination. Cell 152, 1051–1064 (2013).
Helming, K. C., Wang, X. & Roberts, C. W. Vulnerabilities of mutant SWI/SNF complexes in cancer. Cancer Cell 26, 309–317 (2014).
Whitehouse, I. et al. Nucleosome mobilization catalysed by the yeast SWI/SNF complex. Nature 400, 784–787 (1999).
Masliah-Planchon, J., Bieche, I., Guinebretiere, J. M., Bourdeaut, F. & Delattre, O. SWI/SNF chromatin remodeling and human malignancies. Annu. Rev. Pathol. 10, 145–171 (2015).
Gao, X. et al. ES cell pluripotency and germ-layer formation require the SWI/SNF chromatin remodeling component BAF250a. Proc. Natl Acad. Sci. USA 105, 6656–6661 (2008).
Fernandez-Salguero, P. et al. Immune system impairment and hepatic fibrosis in mice lacking the dioxin-binding Ah receptor. Science 268, 722–726 (1995).
Xia, P. et al. Sox2 functions as a sequence-specific DNA sensor in neutrophils to initiate innate immunity against microbial infection. Nat. Immunol. 16, 366–375 (2015).
Xia, P. et al. Glutamylation of the DNA sensor cGAS regulates its binding and synthase activity in antiviral immunity. Nat. Immunol. 17, 369–378 (2016).
Wang, S. et al. Natural killer-like B cells prime innate lymphocytes against microbial infection. Immunity 45, 131–144 (2016).
Wang, S. et al. Transient activation of autophagy via Sox2-mediated suppression of mTOR is an important early step in reprogramming to pluripotency. Cell Stem Cell 13, 617–625 (2013).
Xia, P. et al. IRTKS negatively regulates antiviral immunity through PCBP2 sumoylation-mediated MAVS degradation. Nat. Commun. 6, 8132 (2015).
Xia, P. et al. Insulin-InsR signaling drives multipotent progenitor differentiation toward lymphoid lineages. J. Exp. Med. 212, 2305–2321 (2015).
Acknowledgements
We thank Dr Liang Zhou (University of Florida) for his technical support and critical discussions, Junying Jia and Yan Teng for their technical support. This work was supported by the National Natural Science Foundation of China (31530093, 31429001, 91640203, 91419308, 81601361, 31570872, 31471386 and 31671531), the Strategic Priority Research Programs of the Chinese Academy of Sciences (XDB19030203 and XDA12020219). Youth Innovation Promotion Association of CAS to S.W. and the China Postdoctoral Science Foundation to P.X. (2015M571141).
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P.X., J.L. and S.W. designed and performed experiments, analysed data. P.X. wrote the paper; B.Y. analysed data; Y.D. and Z.X. performed some experiments; Z.-G.H. provided Arid1a flox mice and analysed data; Z.F. initiated the study, organized, designed and wrote the paper.
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Xia, P., Liu, J., Wang, S. et al. WASH maintains NKp46+ ILC3 cells by promoting AHR expression. Nat Commun 8, 15685 (2017). https://doi.org/10.1038/ncomms15685
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DOI: https://doi.org/10.1038/ncomms15685
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