Abstract
We examined the role of a cytosolic phospholipase A2 (cPLA2) in antigen-induced eosinophil infiltration of airways and in airway hyperresponsiveness to methacholine. Inhibition of cPLA2, or blockade of the platelet-activating factor (PAF) receptor, blocked antigen-induced airway hyperresponsiveness and suppressed eosinophil infiltration. Neither cyclooxygenase nor 5-lipoxygenase inhibition had either effect. We show here that, in antigen-sensitized guinea pigs, cPLA2 inhibition prevents both eosinophilic infiltration and subsequent airway hyperresponsiveness after antigen challenge. We also show that this effect is mediated by first-step hydrolysis of membrane phospholipid into lysophospholipid rather than by prostanoid or leukotriene metabolites of arachidonate.
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Acknowledgements
This work was supported by NHLBI grant RO1-HL46368 and by NHLBI SCOR grant HL-56399. S. M. and H. S. were supported by the AstraZeneca Traveling Fellowship
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Myou, S., Sano, H., Fujimura, M. et al. Blockade of eosinophil migration and airway hyperresponsiveness by cPLA2-inhibition. Nat Immunol 2, 145–149 (2001). https://doi.org/10.1038/84244
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DOI: https://doi.org/10.1038/84244
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