Abstract
THE antidiuretic effect of arginine vasopressin (AVP) is mediated by renal-type (V2) receptors linked to adenylyl cyclase1. We report here the cloning of the rat kidney V2 AVP receptor complementary DNA that encodes a 370-amino-acid protein with a transmembrane topography characteristic of G protein-coupled receptors, and with similarity to the Via (hepatic) AVP receptor2 in its seven membrane-spanning domains. Expression of the cloned cDNA in mammalian cells showed specific ligand binding and activity characteristic of the native V2 AVP receptor. The receptor messenger RNA is detected only in the kidney. The human V2 receptor gene has been localized to the long arm of the X chromosome close to the locus for nephrogenic diabetes insipidus, an X-linked recessive disorder characterized by renal resistance to the antidiuretic action of AVP3–6
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Jard, S. Curr. Top. Membranes Transp. 18, 255–285 (1983).
Morel, A., O'Carroll, A.-M., Brownstein, M. J. & Lolait, S. J. Nature 356, 523–526 (1992).
Bode, H. H. & Crawford, J. D. New Engl. J. Med 280, 750–754 (1969).
Bichet, D. G. et al. Kidney Int. 36, 859–866 (1989).
Knoers, N. et al. Hum. Genet. 80, 31–38 (1988).
Knoers, N. et al. Nephron 50, 187–190 (1988).
Saiki, R. K. et al. Science 239, 487–491 (1988).
Kozak, M. Nucleic Acids Res. 12, 857–872 (1984).
Arai, H., Hori, S., Aramori, I., Ohkubo, H. & Nakanishi, S. Nature 348, 730–732 (1990).
Lin, H. Y. et al. Science 254, 1022–1024 (1991).
Juppner, H. et al. Science 254, 1024–1026 (1991).
Yamada, Y. et al. Proc. natn. Acad. Sci. U.S.A. 89, 251–255 (1992).
Jard, S., Barberis, C., Audigier, S. & Tribollet, E. Prog. Brain Res. 72, 173–187 (1987).
Phillips, P. A. et al. Endocrinology 126, 1478–1484 (1990).
Dorsa, D. M., Majumdar, L. A., Petracca, F. M., Baskin, D. G. & Cornett, L. E. Peptides 4, 699–706 (1983).
Manning, M. et al. J. med. Chem. 27, 423–429 (1984).
Kruszynski, M. et al. J. med. Chem. 23, 364–368 (1980).
Konig, M., Mahan, L. C., Marsh, J. W., Fink, J. S. & Brownstein, M. J. Molec. cell. Neurosci. 2, 331–337 (1991).
Kirk, C. J., Verrinder, T. R. & Hems, D. A. FEBS Lett. 83, 267–271 (1977).
Jans, D. A., van Oost, B. A., Ropers, H. H. & Fahrenholz, F. J. biol. Chem. 265, 15379–15382 (1990).
McBridge, O. W. et al. Nucleic Acids Res. 10, 8155–8170 (1982).
Olsen, A. S., McBride, O. W. & Moore, D. E. Molec. cell. Biol. 1, 439–448 (1981).
Frohman, M. A., Dush, M. K. & Martin, G. R. Proc. natn. Acad. Sci. U.S.A. 85, 8998–9002 (1988).
Young, W. S. III, Bonner, T. I. & Brann, M. R. Proc. natn. Acad. Sci. U.S.A. 83, 9827–9831 (1986).
Young, W. S. III, Warden, M. & Mezey, E. Neuroendocrinology 46, 439–444 (1987).
Chen, C. & Okayama, H. Molec. cell. Biol. 7, 2745–2752 (1987).
Holmes, M. C., Antoni, F. A. & Szentendrei, T. Neuroendocrinology 117, 421–423 (1985).
Antoni, F. A. Neuropeptides 4, 413–420 (1984).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Lolait, S., O'Carroll, AM., McBride, O. et al. Cloning and characterization of a vasopressin V2 receptor and possible link to nephrogenic diabetes insipidus. Nature 357, 336–339 (1992). https://doi.org/10.1038/357336a0
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1038/357336a0
This article is cited by
-
Air sac and gill vasotocin receptor gene expression in the air-breathing catfish Heteropneustes fossilis exposed to water and air deprivation conditions
Fish Physiology and Biochemistry (2022)
-
Novel AVPR2 mutations and clinical characteristics in 28 Chinese families with congenital nephrogenic diabetes insipidus
Journal of Endocrinological Investigation (2021)
-
β-Arrestin 2 mediates arginine vasopressin-induced IL-6 induction via the ERK1/2-NF-κB signal pathway in murine hearts
Acta Pharmacologica Sinica (2020)
-
In situ localization of vasotocin receptor gene transcripts in the brain-pituitary-gonadal axis of the catfish Heteropneustes fossilis: a morpho-functional study
Fish Physiology and Biochemistry (2019)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.