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Drosophila Pin1 prolyl isomerase Dodo is a MAP kinase signal responder during oogenesis

Nature Cell Biology volume 3, pages 538–543 (2001)Cite this article

Abstract

The mammalian cis–trans prolyl isomerase Pin1 and its yeast orthologue Ess1/Ptf1 have been implicated in cell cycle control but a correlation between biochemical and physiological functions has not been established conclusively. Pin1 targets the proline residue carboxy-terminal to the phosphorylated threonine or serine residue, which constitutes part of the phosphorylated mitogen-activated protein kinase (MAPK) site PXpT/SP. Here we show that the Drosophila Pin1 homologue, the Dodo protein, is involved in dorsoventral patterning of the follicular epithelium in the egg chamber. Its function is to facilitate the degradation of transcription factor CF2, which requires, a priori, activated epidermal growth factor receptor–MAPK signalling.

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Figure 1: Genomic location of dodo.
Figure 2: Egg chamber defects in the dodo deficiency mutant.
Figure 3: Dodo protein expression in the egg chamber.
Figure 4: Interaction between Dodo and phosphorylated CF2 protein.
Figure 5: The dodo function exacerbates D-raf gain-of-function mutant phenotypes.

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Acknowledgements

We thank D. Spyropoulos, M. Trojanowska and D. Watson for valuable comments on the manuscript; N. Perrimon for providing the hs-D-rafgof flies; G.L.G. Miklos for the gift of the flightless-I transgenic stocks; and X. Zhang of the Medical University of South Carolina Antibody Production Facility for producing antisera. This work is supported by a grant from the National Institutes of Health to T.H. and a grant from the American Heart Association to H.G.d.G.

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Authors and Affiliations

  1. Laboratory of Cancer Genomics, Hollings Cancer Center, Medical University of South Carolina, 86 Jonathan Lucas Street, Charleston, 29425, South Carolina, U.S.A.

    Tien Hsu & Daniel McRackan

  2. Department of Cell Biology and Anatomy, Medical University of South Carolina, 86 Jonathan Lucas Street, Charleston, 29425, South Carolina, USA

    Tien Hsu

  3. Department of Pathology and Laboratory Medicine, Medical University of South Carolina, Charleston, South Carolina, USA

    Timothy S. Vincent

  4. Department of Zoology, University of Hawai'i, Honolulu, Hawai'i, USA

    H. Gert de Couet

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Correspondence to Tien Hsu.

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Hsu, T., McRackan, D., Vincent, T. et al. Drosophila Pin1 prolyl isomerase Dodo is a MAP kinase signal responder during oogenesis. Nat Cell Biol 3, 538–543 (2001). https://doi.org/10.1038/35078508

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