Abstract
Erythropoietin is the primary physiological regulator of erythropoiesis; however, in vitro studies have identified another class of mediators which appear to be important in stimulating erythroid progenitors. These factors have generally been referred to as burst-promoting activities (BPA), because they stimulate the growth of early erythroid progenitors referred to as burst-forming units-erythroid (BFU-E) which give rise to colonies of up to thousands of haemoglobinized cells1,2. We recently reported purification of a burst-promoting activity from medium conditioned by the Mo T-lymphoblast cell line infected with human T-cell lym-photropic virus type II (HTLV-II)3,4. This purified glycoprotein of relative molecular mass (Mr) 28,000 also stimulates colony formation by more mature erythroid precursors (CFU-E) and is therefore referred to as erythroid-potentiating activity (EPA)5. Purified EPA specifically stimulates human and murine cells of the erythroid lineage, unlike murine interleukin-3 (IL-3) which stimulates precursor cells from all haematopoietic lineages6. We report here the isolation of a complementary DNA molecular clone encoding EPA and its use in producing EPA in COS (monkey) cells and CHO (Chinese hamster ovary) cells. We also define the organization of the EPA gene in human DNA.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Dexter, T. M. Nature 309, 746–747 (1984).
Iscove, N. N. in Hematopoietic Cell Differentiation (eds Golde, D. W., Cline, M. J., Metcalf, D. & Fox, C. F.) 37–52 (Academic, New York, 1978).
Westbrook, C. A., Gasson, J. C., Gerber, S. E., Selsted, M. E. & Golde, D. W. J. biol. Chem. 259, 9992–9996 (1984).
Gasson, J. C., Bersch, N. : Golde, D. W. in Stem Cell Physiology (ed. Palek, J.) (Eiss, New York, in the press).
Golde, D. W., Bersch, N., Quan, S. G. & Lusis, A. J. Proc. natn. Acad. Sci. U.S.A. 77, 593–596 (1980).
Ihle, J. N. et al. J. Immun. 131, 282–287 (1983).
Elder, J. H. & Alexander, S. Proc. natn. Acad. Sci. U.S.A. 79, 4540–4544 (1982).
Kawakami, T. G. et al. Nature new Biol. 235, 170–171 (1972).
Arya, S. K., Wong-Staal, F. & Gallo, R. C. Science 223, 1086–1087 (1984).
Ascensao, J. L., Kay, N. E., Earenfight-Engler, T., Koren, H. S. & Zanjani, E. D. Blood 57, 170–173 (1981).
Gillis, S., Crabtree, G. R. & Smith, K. A. J. Immun. 123, 1624–1631 (1979).
Wong, G. G. et al. Science 228, 810–815 (1985).
Gluzman, Y. Cell 23, 175–182 (1981).
Southern, E. M. J. molec. Biol. 98, 503–517 (1975).
Rimm, D. L., Horness, D., Kucera, J. & Blattner, F. R. Gene 12, 301–309 (1980).
Fung, M. C. et al. Nature 307, 233–237 (1984).
Yokota, T. et al. Proc. natn. Acad. Sci. U.S.A. 81, 1070–1074 (1984).
Skup, D. et al. Nucleic Acids Res. 10, 3069–3084 (1982).
Messing, J. & Vieira, J. Gene 19, 269–276 (1982).
Chen, I. S. Y., Quan, S. G. & Golde, D. W. Proc. natn. Acad. Sci. U.S.A. 80, 7006–7009 (1983).
O'Rear, J. J., Mizutani, S., Hoffman, G., Fiandt, M. & Temin, H. M. Cell 20, 423–430 (1980).
Becker, A. & Gold, M. Proc. natn. Acad. Sci. U.S.A. 72, 581–585 (1975).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Gasson, J., Golde, D., Kaufman, S. et al. Molecular characterization and expression of the gene encoding human erythroid-potentiating activity. Nature 315, 768–771 (1985). https://doi.org/10.1038/315768a0
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1038/315768a0
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.
