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SV40 large T shares an antigenic determinant with a cellular protein of molecular weight 68,000

Nature volume 288, pages 167–170 (1980)Cite this article

Abstract

The large T protein coded by the early region of simian virus 40 (SV40) is involved in the induction and maintenance of cell transformation1. It is not clear which, properties of T are important in causing the transformation, since the protein is multifunctional2–9. To clarify the action of T proteins in transformation, we have prepared several monoclonal antibodies directed against different regions of the T molecule. One of these antibodies, DL 3C4, recognizes an antigenic determinant on T that is also present on a host cell protein of molecular weight 68,000. This cross-reactive 68K protein is located within the nucleus of all mammalian cell types examined and has a characteristic granular distribution as shown by immuno-fluorescence. The antigenic determinant recognized by DL 3C4 is resistant to denaturation. Studies on Adeno-SV40 hybrid viruses show that the antigenic site on T is coded for by sequences located between 0.44 and 0.29 on the SV40 map.

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References

  1. Tooze, J. (ed.) The Molecular Biology of Tumor Viruses 2nd Edn, Vol. 2 (Cold Spring Harbor Laboratory, New York, 1980).

  2. Tjian, R., Fey, G. & Graessmann, A. Proc. natn. Acad. Sci. U.S.A. 75, 1279–1282 (1978).

    Article  ADS  CAS  Google Scholar 

  3. Tegtmeyer, P. J. Virol. 10, 591–598 (1972).

    CAS  PubMed  PubMed Central  Google Scholar 

  4. Carroll, R., Hager, L. & Dulbecco, R. Proc. natn. Acad. Sci. U.S.A. 72, 3754–3757 (1974).

    Article  ADS  Google Scholar 

  5. Tjian, R. Cell 13, 165–180 (1978).

    Article  CAS  PubMed  Google Scholar 

  6. Chang, C., Luborsky, S. W. & Mora, P. T. Nature 269, 438–440 (1977).

    Article  ADS  CAS  PubMed  Google Scholar 

  7. Tjian, R. & Robbins, A. Proc. natn. Acad. Sci. U.S.A. 76, 610–614 (1979).

    Article  ADS  CAS  Google Scholar 

  8. Griffen, J. D., Spangler, G. & Livingstone, D. M. Proc. natn. Acad. Sci. U.S.A. 76, 2610–2614 (1979).

    Article  ADS  Google Scholar 

  9. Lane, D. P. & Crawford, L. V. Nature 278, 261–263 (1979).

    Article  ADS  CAS  PubMed  Google Scholar 

  10. Kohler, G. & Milstein, C. Nature 256, 495–497 (1975).

    Article  ADS  CAS  PubMed  Google Scholar 

  11. Shulman, M., Wilde, C. D. & Kohler, G. Nature 276, 269–270 (1978).

    Article  ADS  CAS  PubMed  Google Scholar 

  12. Kennett, R. H., Denis, K. A., Tung, A. S. & Klinman, N. R. Curr. Topics Microbiol. Immun. 81, 77–91.

  13. Lane, D. P. & Robbins, A. K. Virology 86, 182–193 (1978).

    Article  Google Scholar 

  14. Lebowitz, P., Kelly, T. J., Nathans, D., Lee, T. H. & Lewis, A. M. Proc. natn. Acad. Sci. U.S.A. 71, 441–445 (1974).

    Article  ADS  CAS  Google Scholar 

  15. Kelly, T. J. & Lewis, A. M. Jr J. Virol. 12, 653–658 (1973).

    PubMed  PubMed Central  Google Scholar 

  16. Walen, K. H. & Brown, S. W. Nature 194, 406 (1962).

    Article  ADS  CAS  PubMed  Google Scholar 

  17. Burridge, K. Proc. natn. Acad. Sci. U.S.A. 73, 4457–4461 (1976).

    Article  ADS  CAS  Google Scholar 

  18. Renart, J., Reiser, J. & Stark, G. R. Proc. natn. Acad. Sci. U.S.A. 76, 3116–3120 (1979).

    Article  ADS  CAS  Google Scholar 

  19. Collett, M. S., Erikson, E., Purchio, A. F., Brugge, J. S. & Erikson, R. L. Proc. natn. Acad. Sci. U.S.A. 76, 3159–3163 (1979).

    Article  ADS  CAS  Google Scholar 

  20. Laemmli, U. K. Nature 227, 680–685 (1970).

    Article  ADS  CAS  PubMed  Google Scholar 

  21. Kessler, S. W. J. Immun. 115, 1617–1624 (1975).

    CAS  PubMed  Google Scholar 

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Author notes

  1. D. P. Lane

    Present address: Department of Zoology and Cancer Research Campaign Eukaryotic Molecular Genetics Research Group, Imperial College, London, SW7 2AZ, UK

Authors and Affiliations

  1. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York, 11724

    D. P. Lane & W. K. Hoeffler

Authors
  1. D. P. Lane
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  2. W. K. Hoeffler
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Lane, D., Hoeffler, W. SV40 large T shares an antigenic determinant with a cellular protein of molecular weight 68,000. Nature 288, 167–170 (1980). https://doi.org/10.1038/288167a0

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