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Haemorheological Variables in a Rat Model of Hypertriglyceridaemic Obesity and Diabetes

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Abstract

Obesity is a heterogeneous condition of variable aetiology, generally associated with pathologies such as arterial hypertension, hyperlipidaemia, diabetes and cardiac disease. These conditions, either themselves or because of the various treatments used, may further modify blood rheology in an arbitrary manner. Therefore, analyses of changes in the blood rheology induced by obesity in humans have had differing and controversial results. In our laboratory, a model of hypertriglyceridaemic obesity is provided by an inbred rat strain; the β genotype from the IIMb/Fm strain, presenting a syndrome of moderate obesity with apparent peripubertal onset, associated with hypertriglyceridaemia and glucose intolerance that turns into diabetes. The α genotype, originated from the same IIM/Fm stock, represents the control.

The present study describes a comparative analysis of the variables determining the rheological behaviour of the blood in obese and control strains. Our results, agreeing with some other studies performed in humans, confirmed the haemorheological changes associated with obesity, and the fact that these changes became more evident in the presence of pathologies such as diabetes. It appears that triglyceridaemia, cholesterolaemia and hyperglycaemia may influence the rheological behaviour of the cell membrane and this damage may provoke a decrease in erythrocyte deformability and, consequently, hyperviscosity of the blood.

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REFERENCES

  • Allain, C.C., Poon, L.S., Chan, C.S.G., Richmond, W. and Fu, F.O., 1974. Enzymatic determination of total serum cholesterol. Clinical Chemistry, 20, 470-475

    Google Scholar 

  • Banerjee, R., Nageshwaari, K. and Punivani, R.R., 1998. The diagnostic relevance of red cell rigidity. Clinical Hemorheology and Microcirculation, 19, 21-24

    Google Scholar 

  • Bucolo, G. and David, H., 1973. Quantitative determination of serum triglycerides by the use of enzymes. Clinical Chemistry, 19, 476

    Google Scholar 

  • Calderari, S.A., Gonzalez, A., Hechen, A. and Antonio, E., 1981. Obesidad hereditaria de la rata asociada a niveles plasmáticos elevados de triglicéridos. Medicina (B. Aires), 41, 758

    Google Scholar 

  • Calderari, S.A., Gonzalez, A. and Gayol, M., 1987. Spontaneous hypertrigliceridemic obesity and hyperglicemia in an inbred line of rats. International Journal of Obesity, 11, 571-579

    Google Scholar 

  • Craveri, A., Tornaghi, G., Paganardi, L., Passaretti, B. and Scaglioni, S., 1988a. Hemorheological changes in adult and child obesity are related to overweight. Annali Italiani di Medicina Interna, 3, 111-121

    Google Scholar 

  • Craveri, A., Tornaghi, G., Paganardi, L., Ranieri, R., Scaglioni, S., Torchiana, M. and Giovannini, M., 1988b. Hemorheological changes in obesity. Clinical Hemorheology, 8, 723-736

    Google Scholar 

  • Craveri, A., Tornaghi, G., Paganardi, L., Ranieri, R. and Giavardi, L., 1990. Weight loss does not affect the hemorheological and fibrinolytic changes in obesity. Clinical Hemorheology, 10, 541-546

    Google Scholar 

  • Devereux, R.B., Drayer, J.I.M. and Chien, S., 1984. Whole blood viscosity as a determinant of cardiac hypertrophy in systemic hypertension. American Journal of Cardiology, 54, 592-595

    Google Scholar 

  • Dodds, A.J., Boyd, M.J., Allen, J., Benned, E.D., Flute, P.T. and Dormandy, J.A., 1980. Changes in red cell deformability and other haemorheological variables after myocardial infarction. British Heart Journal, 44, 508-511

    Google Scholar 

  • Expert Panel 1980. Expert Panel on blood cell sizing of the International Committee for Standardization in Haematology, 1980. Recommendation for reference method for determination by centrifugation of packed cell volume of blood. Journal of Clinical Pathology, 33, 1-2

    Google Scholar 

  • Expert Panel 1986. Expert Panel on blood rheology of the International Committee for Standardization in Haematology. Guidelines for measurement of blood viscosity and erythrocyte deformability. Clinical Hemorheology, 6, 439-452

    Google Scholar 

  • Ernest, E., Weihmayr, T., Schmid, M., Bumann, M. and Matrai, A., 1986. Cardiovascular risk factors and hemorheology. Physical fitness, stress and obesity. Atherosclerosis, 56, 263-269

    Google Scholar 

  • Ernest, E., Koening, M., Matrai, A. and Keil, U., 1988. Plasma viscosity and hemoglobin in the presence of cardiovascular risk factors. Clinical Hemorheology, 8, 507-515

    Google Scholar 

  • Festing, M. (ed.), 1993. Abbreviated list of inbred strains of rats. In: International Index of Laboratory Animals, (Lion Litho, Carshalton, Surrey), 51-67

  • Hui, D.Y. and Harmony, J.A.K., 1979. Interaction of plasma lipoproteins with erythrocytes. Alteration of erythrocyte morphology. Biochimica et Biophysica Acta, 550, 407-424

    Google Scholar 

  • Juhan, I., Buonocore, M., Jouve, R., Vague, P., Moulin, J.P. and Vialettes, B., 1982. Abnormalities of erythrocyte deformability and platelet aggregation in insulin-dependent diabetics corrected by insulin “in vivo” and “in vitro”. Lancet, 1, 535

    Google Scholar 

  • Kikuchi, Y., Arat, T. and Koyarna, T., 1983. Improved filtration method for red cell deformability measurement. Medical Biological Engineering and Computing, 42, 127-135

    Google Scholar 

  • Le-Devehat, C., Khodabandehlou, T. and Dougny, M.L.L., 1992. Hemorheological parameters in isolated obesity. Diabetes and Metabolism, 18, 43-47

    Google Scholar 

  • Lipovac, V., Gavella, M., Crnek, S. and Rubinjoni, Z., 1990. Erythrocyte sialic acid and filterability in type 2 diabetic patients. Clinical Hemorheology, 10, 477-483

    Google Scholar 

  • Martinez, M., Vaya, A., Marti, R., Gill, L., Lluch, J., Carmena, R. and Aznar, J., 1996. Effect of HMG-CoA reductase inhibitors on red blood cell membrane lipids and haemorheological parameters in patients affected by familial hypercholesterolaemia. Haemostasis, 26, 171-176

    Google Scholar 

  • Martinez, M., Vaya, A., Marti, R., Gill, L., Lluch, J., Dalmau, J. and Aznar, J., 1998. The cholesterol/phospholipid ratio of the erythrocyte membrane in children with familial hypercholesterolaemia. Its relationship with plasma lipids and red blood aggregability. Clinical Hemorheology and Microcirculation, 18, 259-265

    Google Scholar 

  • McMillan, D.E., Utterback, N.G. and LaPuma, J., 1978. Reduced erythrocyte deformability in diabetes. Diabetes, 27, 895

    Google Scholar 

  • Petit, K.L., Hunt, W.B., George, S.J. and Barnes, A.J., 1996. Is impaired red cell filtration in diabetics due to a small abnormal sub-population of cells? Clinical Hemorheology, 16, 479-485

    Google Scholar 

  • Poggi, M., Palareti, G., Biagi, R., Legnani, C., Parenti, M., Babini, A.C., Baraldi, L. and Coccheri, S., 1994. Prolonged very low calorie diet in highly obese subjects reduces plasma viscosity and red cell aggregation but not fibrinogen. International Journal of Obesity and Related Metabolic Disorders, 18, 490-496

    Google Scholar 

  • Rabasa, S.L. and Font, M., 1963. Cría y selección de la rata blanca. Ciencia e Investigación, 19, 133-137

    Google Scholar 

  • Reid, H.L., Barnes, A.J., Lock, P.J., Dormandy, J.A. and Dormandy, T.L., 1976. A sample method for measuring erythrocyte deformability. Journal of Clinical Pathology, 29, 855-858

    Google Scholar 

  • Rillaerts, E., Vansant, G., Van Gaal, L. and De Leeuw, I., 1989. Effect of weight reduction on blood viscosity parameters in obese women. Clinical Hemorheology, 9, 983-987

    Google Scholar 

  • Romero, L.E., Brandani, L., Graf, S., Velikosky, G., Espinoza, D., Sanchez, R. and Arnentano, R.L., 1997. Análisis de la viscosidad sanguínea en pacientes hipertensos. Revista Argentina de Bioingenieria, 312, 30-34

    Google Scholar 

  • Serrano Rios, M., 1998. Relationship between obesity and the increased risk of major complications in non-insulin-dependent diabetes mellitus. European Journal of Clinical Investigation, 28, 14-18

    Google Scholar 

  • Sloop, G.D. and Garber, D.W., 1997. The effects of low-density lipoprotein and high-density lipoprotein on blood viscosity correlate with their association with risk of atherosclerosis in humans. Clinical Science, 92, 473-479

    Google Scholar 

  • Sloop, G.D. and Mercante, D.E., 1998. Opposite effects of low-density and high-density lipoprotein on blood viscosity in fasting subjects. Clinical Hemorheology and Microcirculation, 19, 197-203

    Google Scholar 

  • Stuart, J., George, A.J. and Davies, A.J., 1981. Haematological stress syndrome in atherosclerosis. Journal of Clinical Pathology, 36, 464-467

    Google Scholar 

  • Stuart, J., Kenny, M.W., Aukland, A., George, A.J., Neuman, V., Shapiro, L.M. and Cove, D.H., 1983. Filtration of washed erythrocytes in atherosclerosis and diabetes mellitus. Clinical Hemorheology, 3, 23-30

    Google Scholar 

  • Sutera, S.P., Gardner, R.A., Boylan, C.W., Carroll, G.L., Chang, K.C., Marvel, J.S., Kilo, C., Gonen, B. and Williamson, J.R., 1985. Age-related changes in deformability of human erythrocytes. Blood, 65, 275-282

    Google Scholar 

  • Terranova, R., Alberghina, M. and Carnazzo, G., 1985. Lipid composition and fluidity of the erythrocyte membrane in the elderly. La Ricerca in Clinical e in Laboratorio, 15, 327-334

    Google Scholar 

  • Trinder, P., 1969. Determination of glucose in blood using glucose oxidase with an alternative oxygen acceptor. Annals of Clinical Biochemistry, 6, 24-27

    Google Scholar 

  • Tunbridge, M.G., 1981. Factors contributing to deaths of diabetics under fifty years of age. Lancet, 2, 569-575

    Google Scholar 

  • Vague, I., Vague, P.H., Alessi, M.C., Badier, C., Vladier, J., Aillaud, M.F. and Atlan, C., 1987. Relationship between plasma insulin, triglycerides, body mass index and plasminogen activator inhibitor. Diabetes and Metabolism, 13, 331-336

    Google Scholar 

  • Vaya, A., Martinez, M., Dalmau, J. and Aznar, J., 1998. Hemorheological changes in children with polygenic hypercholesterolaemia. Clinical Hemorheology and Microcirculation, 19, 159-262

    Google Scholar 

  • Weinschelbaum-Jairala, S., Schreier, L., Ochoa, E., Gayol, M., Proto, E., Posadas, M., Wikinski, R. and Calderarri, S.A., 1995. Variaciones de biomas, lipidemia, presión arterial y glucemia en dos líneas endocriadas de ratas: obesa y sin sobrepeso. Medicina (B. Aires), 55, 515-516

    Google Scholar 

  • Wintrobe, M.M., 1967. Clinical Hematology, (Lea & Febiger, Philadelphia), 429-430

    Google Scholar 

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Authors and Affiliations

  1. Cátedra de Biofísica, Facultad de Ciencias Médicas, Santa Fe 3100, 2000, Rosario, Argentina

    G.N. Herńandez, C. Dabin & M.L. Rasia

  2. Facultad de Ciencias Médicas, Santa Fe 3100, 2000, Rosario, Argentina

    M. del C. Gayol

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  1. G.N. Herńandez
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  2. C. Dabin
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  3. M. del C. Gayol
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  4. M.L. Rasia
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Herńandez, G., Dabin, C., del C. Gayol, M. et al. Haemorheological Variables in a Rat Model of Hypertriglyceridaemic Obesity and Diabetes. Vet Res Commun 26, 625–635 (2002). https://doi.org/10.1023/A:1020920820767

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