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Differential Expression of Somatostatin Receptors in Medulloblastoma

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Abstract

Object. Somatostatin receptors have been found on a variety of tumours like neuroendocrine breast or brain tumours. Their detection opens new diagnostic and therapeutic paths. The aim of this work was to investigate their expression in medulloblastomas.

Methods. Using both techniques, reverse transcriptase-polymerase chain reaction and immunohistochemistry, we analysed mRNA of different subtypes of somatostatin receptors in 15 medulloblastomas and the localisation of the subtype SSTR2 receptor at the cellular level in 13 medulloblastomas. All five subtypes mRNA were variably expressed in each medulloblastoma. The signal obtained after Southern blotting for SSTR2 receptor amplification was the highest as compared to the signal obtained for the other receptor subtypes. Immunostaining for SSTR2A receptor was present in every tumour specimen and was specifically located to the cellular membrane of neoplastic cells. No staining was identified at the level of peritumoral veins.

Conclusion. The evidence of predominant expression of SSTR2 receptors in medulloblastomas opens interesting prospects for their diagnosis and therapy.

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Authors and Affiliations

  1. Service de Neurochirurgie B, Hôpital Neurologique et Neurochirurgical Pierre Wertheimer, France

    Jacques Guyotat, Philippe Bret & Francesco Signorelli

  2. Faculté de Médecine Laennec, Inserm U 433, Rue Guillaume Paradin, France

    Jacques Champier, Christine Brisson, Marie France Belin & Michelle Fevre Montange

  3. Service de Neuropathologie, Hôpital Neurologique et Neurochirurgical Pierre Wertheimer, Lyon, France

    Ghislaine Saint Pierre & Anne Jouvet

Authors
  1. Jacques Guyotat
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  2. Jacques Champier
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  3. Ghislaine Saint Pierre
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  5. Philippe Bret
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  9. Michelle Fevre Montange
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Guyotat, J., Champier, J., Saint Pierre, G. et al. Differential Expression of Somatostatin Receptors in Medulloblastoma. J Neurooncol 51, 93–103 (2001). https://doi.org/10.1023/A:1010624702443

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